35 results about "Vpr Protein" patented technology

Novel packaging cell lines which produce recombinant retrovirus, free of detectable helper-virus are disclosed. Also disclosed are methods of making the cell lines and methods of producing recombinant retroviruses from the cell lines. Retroviruses produced by the cell lines include lentiviruses, such as HIV, capable of transfering heterologous DNA to a wide range of non-dividing cells. The packaging cells contain at least three vectors which collectively encode retroviral gag, pol, and env proteins, wherein the gag and pol genes are separated, in part, onto two or more different vectors. This is made possible by fusing Vpr or Vpx to pol proteins separated from gag so that the proteins are targeted to assembling virions. Among other advantages, the packaging cells provide the benefit of increased safety when used in human gene therapy by virtually eliminating the possibility of molecular recombination leading to production of replication-competant helper virus.

Positive identification of local inhabitants plays an important role in modern military, police and security operations. Since terrorists use all means to masquerade as local inhabitants, the identification of terrorist or hostile suspects becomes an increasingly complicated task. The instant software solution will assist military, police and security forces in the identification of suspects using Voice Print Recognition (VPR) technology. Our VPR software will compare and recognize, or match, specific voice samples with stored, digital voice models (voice prints) for the purpose of establishing or verifying identity. VPR software will support an operator's decision and situational awareness through the verification of a person's identity (for instance: remote access control), but more importantly will assist in the identification of suspect individuals (identifying suspects among a large group of captured individuals). This second application is critical for the modern counter and anti-terrorist operations environment. The VPR system will be easy to use, fast, and helpful to users with minimal operational training. The VPR system will provide a method, as practiced on or via access to a computing device, which includes software for acquisition of voice records, storage of such records, identification algorithms, user and software interfaces. The system will also have server and client applications in its implementation.

Nucleic acids are disclosed encoding fusion proteins comprising the amino acid sequence of at least four non-attenuated HIV proteins selected from the group consisting of Vif, Vpr, Vpu, Vpx, Rev, Tat, and Nef, wherein the fusion protein does not contain a specific cleavage sequence for a cellular protease. Also disclosed are vectors comprising the nucleic acids and methods of preparing the fusion protein by transfecting a host cell with the nucleic acids or with the vectors containing the nucleic acids, expressing the fusion proteins, and recovering the fusion proteins.

The invention discloses a recombinant strain of Bacillus subtilis, a preparation method and application thereof, and belongs to the field of biotechnology. The recombinant strain provided by the present invention is obtained by knocking out or inactivating ten protease genes in Bacillus subtilis, these ten protease genes are: nprE, nprB, aprE, mpr, bpf, epr, vpr, wprA, spollAC and srfAC. In the present invention, the aforementioned 10 protease genes are knocked out through the method of homologous recombination, and the obtained Bacillus subtilis recombinant strain grows fast and has high expression of foreign protein. Therefore, the recombinant strain can be used as a host for high-efficiency expression of foreign proteins.

The present invention provides monoclonal antibodies to HIV-1 Vpr and hybridoma cell lines that produce the monoclonal antibodies to HIV-1 Vpr. Methods for use of such antibodies in the detection of HIV-1 infection are also provided.

Provided is a wheel drive system for aircraft RS including a motor 2 that is connected to a wheel 12, and provided with two voltage supply lines L1 and L2 for supplying the voltages Vta and Vpr with varying the length of a winding 21 to be energized; a voltage supply unit for supplying the voltage Vta and Vpr to the two voltage supply lines L1 and L2; and a control unit 8 for controlling the voltage supply unit, wherein the control unit 8 is configured to select one of the two voltage supply lines L1 and L2, and supply the voltage Vta or Vpr, by controlling the voltage supply unit.

This invention provides novel methods for identifying agents that inhibit HIV infection. The anti-HIV agents are identified by screening test compounds for ability to modulate a biological activity of isopeptidase T (IsoT), e.g., its isopeptidase activity or its binding to another molecule such as viral protein R (Vpr). Such IsoT modulators can be further examined for their activity in inhibiting an activity indicative of HIV infection or HIV replication. These novel anti-HIV agents are useful in the prevention or treatment of HIV infection and conditions associated with or caused by HIV infection.

The invention relates to APS method for shared VP- ring in ring network with ATM technology, which comprises: sending and receiving the APS cell defined with I.630 as reference and contained alert information and node space code address to detect logic link group state, realizing said protective switch to logic business with millisecond-level response speed; wherein, the switch is realized by modifying route list; when some node takes APS to self-ring business, the business of this ring also be automatic protection switched to match ring. This invention has less damage to business and high utility for bandwidth, and can match with APS of SDH.

The invention discloses a method for extracting enhanced VPR (viral protein regulatory) protein and plasma free nucleic acid. An amino acid sequence of the enhanced VPR protein is obtained by the mutation of the amino acid sequence shown in SEQ ID NO:1; the mutation at least comprises one of following mutation sites: a 55th site, a 102nd site, a 134th site and a 166th site; the mutation of tyrosine at the 55th site is phenylalanine; the mutation of serine at the 102nd site is threonine; the mutation of serine at the 134th site is isoleucine; the mutation of serine at the 166th site is threonine; or, the amino acid sequence of the enhanced VPR protein has the mutation sites in the amino acid sequence with mutation, and has more than 98% homology with the amino acid sequence with mutation. The enhanced VPR protein has the advantage that the activity is higher at normal temperature, so that the heating is not required in the plasma free nucleic acid extraction process, and the degradationof nucleic acid is reduced.