76 results about "Sphingomonas azotifigens" patented technology

The invention relates to Sphingomonas sp. TP-5 and a method and an application of the same for producing welan gum. A strain of the invention is prepared by separating and acclimating sugar-contained wastewater in a molasses plant, has a systematic name of Sphingomonas sp. and a preservation number of CGMCCNo.3097, not only can grow in nutrient media of beef broth, LB and nutrient agar, but also can grow in a sugar-contained inorganic-salt culture medium. The TP-5 strain can be fermented in the sterile culture medium containing sugar, inorganic salt and water at a temperature condition of 28-37 DEG C; ymotic fluid is extracted to obtain the welan gum achieving a temperature-resistant limit value of 150 DEG C; and potassium chloride in different concentrations is added so that a welan gum solution remarkably improves the viscosity, enhances the temperature stability, does not vary the viscosity with temperature and presents stronger temperature tolerance, thereby being applied to seawater drilling mud and a high salinity reservoir viscous water flooding system.

The present invention relates to a Lactuca sativa L. var. longifolia Lam seed designated as 41-49 RZ, which has leaves with a hollow main vein, broad base leaves, and exhibits resistance against downy mildew (Bremia lactucae Regel), currant-lettuce aphid (Nasonovia ribisnigri), corky root (Sphingomonas suberifaciens) and lettuce mosaic virus (LMV). The present invention also relates to a Lactuca sativa L. var. longifolia Lam plant produced by growing the “41-49 RZ” seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-49 RZ.

The present invention relates to a Lactuca sativa L. var. longifolia Lam seed designated as 41-52 RZ, referred to as Nirvanus, which exhibits resistance against downy mildew (Bremia lactucae Regel), currant-lettuce aphid (Nasonovia ribis-nigri), corky root (Sphingomonas suberifaciens) and lettuce mosaic virus (LMV), the representative seed having been deposited with the National Collections of Industrial, Marine and Food Bacteria (NCIMB) in Bucksburn, Aberdeen AB21 9YA, Scotland, UK and assigned NCIMB Acession No. 41326. The present invention also relates to a Lactuca sativa L. var. longifolia Lam plant produced by growing the 41-52 RZ (Nirvanus) seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-52 RZ.

The invention relates to a Sphingomonas sp. T-3 and a method for producing biological polysaccharide and poly-beta-hydroxy-butanoic acid by co-fermentation. The collection number of the Sphingomonas sp. strain T-3 is CGMCC No.10150. The method comprises the following steps: carrying out activating culture on the strain T-3, inoculating into a seed culture medium, inoculating a seed solution into the fermentation culture medium, and culturing; and after the fermentation finishes, diluting with distilled water, heating, carrying out membrane separation to obtain a supernate and a thallus precipitate; regulating the pH value of the supernate to 3.0 or so with hydrochloric acid, regulating the pH value of the obtained precipitate to neutrality with NaOH, and drying to obtain the biological polysaccharide; and drying the thallus precipitate, extracting with chloroform, and drying the supernate to obtain the poly-beta-hydroxy-butanoic acid. In the strain T-3 fermentation process, abundant poly-beta-hydroxy-butanoic acid is accumulated in the cells, and abundant biological polysaccharide is secreted into the culture medium; and the two obtained products can be widely used in the fields of industry, food, medical treatment and the like.

The invention discloses a device and method for rapid film culturing of wastewater biofilms, and belongs to the technical field of wastewater treatment. For the problems of long film culturing time, weak firmness and easy falling of the wastewater biofilms in the prior art, the invention provides the device and method for the rapid film culturing of the wastewater biofilms. The device for the rapid film culturing of the wastewater biofilms comprises an MBBR, a water inlet tank is connected at the front end of the MBBR through a dosing pump, a sedimentation tank is connected at the rear end of the MBBR, an air pump supplies air for the MBBR through a pipeline, and an aerator is arranged at the bottom of the MBBR. The method for the rapid film culturing of the wastewater biofilms comprises the following steps: (1) preparing a filler with immobilized red sphingomonas; (2) carrying out aeration treatment; (3) adding the filler in the MBBR which is aerated, and adding a certain amount of the filler to an original reactor; (4) maintaining the aeration rate and a corresponding pH value until the required biofilms are obtained. By adopting the device and method for the rapid film culturing of the wastewater biofilms, the effects of the rapid film culturing speed, firm biofilms and low falling possibility can be realized.

The invention provides a Sphingomonas sp., an extracellular product thereof, and a preparation method. The sphingomonas sp. Is Sphingomonas sp. JA. Deposited as CCTCC NO: M 2017211. MS-001-A-04 strain. The invention also discloses the application of the extracellular product of Sphingomonas sp. In skin cell proliferation, repair and anti-aging, as well as an anti-aging preparation containing the extracellular product of Sphingomonas sp. For promoting cell proliferation and / or migration.

Belonging to the immobilized enzyme field, the invention discloses a preparation method for an immobilized 3-phenoxy benzoic acid degrading enzyme. The method includes the steps of: (1) centrifuging a Sphingomonas sp. SC-1 fermentation liquid, collecting thalli, mixing the thalli with a Tris-HCl buffer solution evenly, then conducting ultrasonic crushing under ice water bath, performing centrifugation to remove intracellular enzyme, adding the Tris-HCl buffer solution to cell debris deposits to a volume before crushing to make an enzyme solution; and (2) mixing the enzyme solution with a sodium alginate solution in certain proportion, dripping the mixed solution in a calcium chloride solution uniformly to perform embedding immobilization. The immobilized 3-phenoxy benzoic acid degrading enzyme prepared by the invention has high enzyme activity under a wide temperature range (4DEG C-40DEG C) and a wide pH range (5.0-9.0), and has strong continuous reaction performance. The preparation method is simple, and the production cost is low.

The invention provides a compound microbial agent for straw turnover, and a preparation method and application of the compound microbial agent. The compound microbial agent comprises fungi and bacteria, wherein the bacteria include lactic acid bacteria, Acetobacter pasteurianus and Sphingomonas xenophaga. The compound microbial agent can quickly compost straws in about 15 days, meanwhile, the nutrient substances of soil are increased, the content of total nitrogen, available phosphorus, organic matters and rapidly available potassium is increased, and the compound microbial agent is favorablefor the growth of crops, inhibits reproduction of harmful bacteria and reduces accumulation of organic acid.

The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.

The invention relates to a Sphingomonas sp. collected under CGMCC No. 11031. The Sphingomonas sp. can be prepared into an HJY bacterial agent applied to enhancing the degrading of residual Chlorpyrifos in Allium tuberosum; compared to an existing technique of degrading the residue of the pesticide Chlorpyrifos in crops, the Sphingomonas sp. can set at roots of Allium tuberosum for a long time and enhance the activity of crops to degrade the residual Chlorpyrifos; at the premise of not affecting the quality of crops, pesticide residue is efficiently degraded, environment protection is given, and the Sphingomonas sp. is suitable for wide popularization and application.

The invention relates to a method for degrading a petroleum component by utilizing nano bamboo charcoal reinforcing functional microorganism and application of the method. The functional microorganism is sphingomonas sp. GY2B; the petroleum component includes polycyclic aromatic hydrocarbon (phenanthrene) and crude oil; 50 to 200mg / L of nano bamboo charcoal can be added to obviously improve the degrading capacity of the functional strain GY2B on petroleum component in water; the sodium alginate embedding method is adopted to cure nano bamboo charcoal and the functional microorganism GY2B to be applied to biological treatment of wastewater containing petroleum component and biological repair of water polluted by petroleum.

The invention provides a sphingomonas engineering bacterium as well as a construction method and application thereof. According to the invention, Sphingomonas sp. T-3 is used as an original strain; efficient transcription of three key genes for synthesis of Sanzan gum is realized by utilizing a gene overexpression method, an engineering strain MH-10 is constructed, the yield of microbial polysaccharide (Sanzan gum) is effectively increased by optimizing fermentation conditions, and the engineering strain MH-10 has a wide industrial application value.

The invention discloses a composition for reducing skin wrinkles and a cosmetic product thereof, and belongs to the technical field of cosmetics. The invention aims to provide a composition, which iscapable of effectively scavenging free radicals, strongly resisting oxidation, promoting collagen synthesis, and delaying skin aging. The composition mainly comprises the following components in partsby weight: 5-10 parts of a ginseng root extract, 2-7 parts of a myrciaria dubia fruit extract, 2-6 parts of a sphingomonas fermentation product extract and 0.5-3 parts of a buckbean leaf extract.

The invention discloses astaxanthin synthetase of sphingomonas, an encoding gene of astaxanthin synthetase and a method for genetic manipulation of sphingomonas. The invention verifies that the sphingomonas can be used for producing astaxanthin, a biological synthesis way capable of producing the astaxanthin is contained, an MEP way and a carotenoid synthesis way are used for producing the astaxanthin in the sphingomonas, and the fact that in the sphingomonas, crtE and crtZ having relatively low homology with the known genes have the function of synthesizing the astaxanthin, can be further proven. Both the enzyme and the encoding gene thereof required by the astaxanthin biosynthetic way of the sphingomonas are not reported in the prior art, so that more resources are provided to the biosynthetic metabolism and transformation of the carotenoid. Genetic manipulation can be carried out on the sphingomonas by taking pBBR1MCS2 as a carrier and taking EGFP as a reporter gene, so that a foundation is laid for the genetic modification of the environmental microorganism of the type and the finding of the degradation mechanisms.

The invention discloses a pigment-free low-molecular weight Welan gum production strain. Sphingomonas sp. HT-1 (CCTCC NO: M2012062) is taken as an original strain to respectively construct a pigment-deficient and decapsulated-structure sphingomonas genetically engineered strain WG-1, a pigment and degrading enzyme gene-deficient sphingomonas engineered strain WG-2, and a pigment and degrading enzyme gene-deficient and decapsulated-structure sphingomonas genetically engineered strain WG-3. The yield of the Welan gum produced by the sphingomonas strain WG-2 in the invention is increased by 10% to 30% compared with the original strain, reaching 35 to 45g / L, and the molecular weight is not significantly changed (Mn: 10000 to 20000kDa). The sphingomonas strain WG-1 and the sphingomonas strain WG-3 in the invention are fermented to produce low-molecular weight Welan gum (Mn: 500 to 1000kDa), and the yield of the Welan gum produced by the decapsulated-structure strain WG-3 is increased by 20%to 30% compared with the strain WG-1, reaching 20 to 25g / L. The strain constructed by the invention can obtain Welan gum products of different molecular weight ranges, and significantly improves thefermentation level of Welan gum.

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

The invention discloses a sphingomonas sanxanigenens and use thereof. Fermentation liquid of the strain or a produced exopolysaccharide composition can be used as a biological flocculant, the functional component of the strain is a novel exopolysaccharide, and the exopolysaccharide is heteropolysaccharide mainly composed of glucose, galacturonic acid, mannose and guluronic acid. The exopolysaccharide has an effective working concentration as low as 4 mg / L and the used fermentation liquid of the sphingomonas sanxanigenens HL-1 has the same action effect. The microbial flocculant has advantages of small dosage, no toxicity and secondary pollution, a good flocculation effect, a wide pH range of use environment, good heat stability and the like, has a flocculation rate capable of reaching 98% when the microbial flocculant is diluted to the polysaccharide content of 4 mg / L in a flocculation system during use, and has remarkable technical economy.

The invention belongs to identification of microorganisms, in particular to a molecular marker used for identification of Sanzan gum and synthesis bacteria thereof, and preparation and application ofthe molecular marker. Sphingomonas sp.CGMCC No.1650 or genome DNA of CGMCC No.19480 is used as a PCR amplification formwork, a primer 1 having a sequence being SEQ No.5 and a primer 2 having a sequence being SEQ No.6 are used as primers, PCR amplification is performed, and the molecular marker is obtained. The problems that when a compounding glue system of the Sanzan gum, gellan gum and welan gumis used, only through other operations of cloning and sequencing and the like, the three gum bodies can be mutually separated, and the step is tedious, can be solved. The molecular marker has the advantages that the Sanzan gum can be identified from medium and light processed products containing the Sanzan gum, and is short in detection time and high in accuracy.

The invention provides a stable suspension system, a preparation method thereof and cosmetics, and relates to the field of cosmetics. The stable suspension system comprises the following components in percentage by mass: at least one of0.1-6% of an acidic substance and 0.1-20% of an ionic substance, 0.1-5% of a thickening agent, 1-40% of grease and the balance of water, wherein the thickening agent comprises at least one of agar, agarose, gellan gum and a sphingomonas fermentation extract. The preparation method of the stable suspension system comprises the following steps: mixing the thickening agent with water to form a water phase; mixing grease with a water phase to form an emulsion; and mixing at least one of an acidic substance and an ionic substance with the emulsion to obtain the stable suspension system. The invention further provides a cosmetic, and the raw materials of the cosmetic comprise the stable suspension system. Tests show that the stable suspension system has good stability in a low pH environment and / or high ion concentration, the preparation method is simple and easy to operate, and the stable suspension system can be applied to cosmetics.

The invention discloses Sphingomonas zeae E18 and an application thereof in biological deodorization, and belongs to the technical field of biology. The preservation number of the Sphingomonas zeae E18 is CCTCC NO: M 2020193. The strain is high in deodorization efficiency, and a biological deodorization microbial agent prepared from the Sphingomonas zeae E18 has obvious degradation effects on odors generated by restaurant and kitchen waste, and has relatively good application potential.

The present invention relates to a Lactuca sativa seed designated 41-106 RZ, which exhibits resistance to downy mildew (Bremia lactucae) races Bl:1-28, Ca-I to Ca-VIII, and strain FR10.021, currant-lettuce aphid (Nasonovia ribisnigri), lettuce mosaic virus (LMV), and to corky root rot (Sphingomonas suberifaciens), as well as broad base leaves. The present invention also relates to a Lactuca sativa plant produced by growing the 41-106 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-106 RZ.

The invention provides a sphingomonas engineering bacterium and its construction method and application. The present invention uses Sphingomonas sp.T-3 as the starting strain, utilizes the gene overexpression method to realize the high-efficiency transcription of three key genes in the synthesis of sanzan gum, constructs the engineering strain MH-10, and effectively improves the microbial polysaccharide by optimizing the fermentation conditions. (Sanzan glue) has a wide range of industrial application value.

The invention belongs to microbial fermentation and particularly relates to sphingomonas azotifigens and an application in preparation of gellan gum. The application comprises the following process steps: a sterilized fermentation culture medium containing a carbon source, a nitrogen source and essential nutritional substances is inoculated with sphingomonas azotifigens CGMCC No.14239; the pH andtemperature of a culture solution are adjusted, and ventilated stirring fermentation is performed; when the viscosity of the culture solution does not increase or the fermentation cycle is smaller than or equal to 50 h, fermentation ends. The technical problems that the gellan gum whiteness is low, follow-up decolorization treatment is required and the process yield is low in the prior art are solved, and the prepared gellan gum product has the advantages of good whiteness, high process yield and the like.

The invention discloses preparation of a sphingomonas fermentation extract and application to a printing and dyeing refining agent. The preparation method of the extract comprises the following steps:adding water and kieselguhr into sphingomonas fermentation broth, stirring and performing frame filtering; discarding the filter cake; collecting the filtrate and performing ultrafiltration with a hollow fiber ultrafiltration membrane; washing with deionized water; adjusting the pH of the final concentrated solution, and adsorbing with cation exchange resin; then eluting by adopting a NaCl solution as eluant; collecting the eluant; performing rotary evaporation concentration and vacuum drying to obtain a sphingomonas fermentation broth extract. The prepared sphingomonas fermentation broth extract is applied to the printing and dyeing refining agent and can effectively remove impurities such as grease and wax in the raw material fiber; moreover, the use of harmful substances is avoided, and the usability and environmental protection property of the printing and dyeing refining agent are improved.

The invention discloses a noni sphingomonas xenophaga preserving culture medium. The culture medium is prepared from 0.8% of dry powder of nutrient broth, 0-0.5% of agar and 1-5% of a noni dried fruit aqueous solution, wherein the volume of the noni dried fruit aqueous solution is settled to be 1000ml, and the pH is adjusted to be 4.0-6.0; the preparation method of the 1-5% of noni dried fruit aqueous solution comprises the following steps of taking 10.0-50.0g of dried fruits, adding 1000ml of water, boiling over the dried fruits and the water for 10 minutes, and replenishing water to 1000ml. After the noni sphingomonas xenophaga cultured on a traditional nutrient agar culture medium is preserved for 15d, the phenomenon that the preserved noni sphingomonas xenophaga is inactive during inoculation appears. According to the preserving culture medium disclosed by the invention, the preserving time of the noni sphingomonas xenophaga can be prolonged, the longest preservation time can achieve 70d, compared with the preservation time of an ordinary nutrient agar culture medium, the preservation time of the noni sphingomonas xenophaga preserving culture medium is prolonged by 3.7 times, and the purpose of long-term preservation is achieved.