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Sphingomonas sanxanigenens and use thereof

A sphingomonas, strain technology, applied in bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problems of high cost of biological flocculants, complicated extraction steps, consumption of a large amount of solvent, etc. Remarkable technical economy, wide pH range of use environment and less dosage

Active Publication Date: 2021-07-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The finished bioflocculant can be prepared by extracting the active ingredients of the bioflocculant, but the extraction steps of the polysaccharide-based flocculant are relatively complicated, consume a large amount of solvent (ethanol) and generate more waste water, resulting in a higher cost of the bioflocculant

Method used

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  • Sphingomonas sanxanigenens and use thereof
  • Sphingomonas sanxanigenens and use thereof
  • Sphingomonas sanxanigenens and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] This example is used to illustrate Sphingomonas Sphingomonas sanxanigenes The identification method and result of HL-1, its steps include:

[0028] (1) Extraction of total bacterial genome DNA: Take 200 μL of HL-1 bacterial liquid from the strain preservation tube into LB medium, put it in a shaking table at 30°C and 200 rpm for 24 h, and extract the total DNA with a genomic DNA extraction kit. DNA.

[0029] (2) PCR amplification of bacterial 16S rDNA: Use bacterial universal primers 27F and 1492R, detect the amplified product by 1% agarose gel electrophoresis, and send it to a sequencing company for sequencing identification.

[0030] Reaction system: ddH 2 O: 20 μL

[0031] High-fidelity DNA polymerase and mixed substrate: 25 μL

[0032] Primer 1: 2 μL

[0033] Primer 2: 2 μL

[0034] Template: 1 μL

[0035] Reaction conditions: pre-denaturation at 95°C for 3 min, denaturation at 95°C for 15 s, annealing at 56°C for 15 s, extension at 72°C for 30 s / 1kb, and ext...

Embodiment 2

[0038] This example is used to illustrate Sphingomonas Sphingomonas sanxanigenes HL-1 produces the cultivation method of biological flocculant, and its steps comprise:

[0039] (1) Take 200 μL from the strain preservation tube Sphingomonas sanxanigenes Put the HL-1 bacterial liquid into the Erlenmeyer flask containing the strain activation medium, put it into a shaker at 30°C and 200 rpm for 24 h, dip a small amount of bacterial liquid with an inoculation loop and streak on the strain activation plate, and incubate at 30 Cultivate in an incubator for 48 h.

[0040] (2) Use an inoculation loop to pick a well-growing single colony on the activation plate and inoculate it into a 250 mL Erlenmeyer flask containing 50 mL of seed medium, and culture it on a shaker at 30°C and 200 rpm for 24 h.

[0041] (3) The cultured seed solution was transferred into a 250 mL Erlenmeyer flask containing 50 mL of fermentation medium at an inoculation volume of 6 % (v / v), and placed in a shaker a...

Embodiment 3

[0046] This example is used to illustrate Sphingomonas Sphingomonas sanxanigenes HL-1 produces the extraction method of extracellular polysaccharide, and its step comprises:

[0047] (1) Extraction of crude exopolysaccharide: put the fermentation broth in Example 2 in a water bath at 80°C for 20 minutes, dilute it with distilled water in equal volume, and centrifuge at 8000 r / min for 30 minutes to remove bacteria, collect the supernatant, concentrate and add 3 times the volume of 95% ethanol, mix well, put it in the refrigerator at 4°C overnight, centrifuge at 8000 r / min for 30 min to remove the supernatant, repeat several times, collect the precipitate, put it in an oven at 80°C to dry to constant weight, and obtain Crude exopolysaccharide.

[0048](2) Remove protein: Dissolve an appropriate amount of crude product in 100 mL distilled water, heat and stir until it dissolves, add 20 mL of a solution of chloroform: n-butanol = 4:1, shake for 30 min, and centrifuge at 8000 r / mi...

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Abstract

The invention discloses a sphingomonas sanxanigenens and use thereof. Fermentation liquid of the strain or a produced exopolysaccharide composition can be used as a biological flocculant, the functional component of the strain is a novel exopolysaccharide, and the exopolysaccharide is heteropolysaccharide mainly composed of glucose, galacturonic acid, mannose and guluronic acid. The exopolysaccharide has an effective working concentration as low as 4 mg / L and the used fermentation liquid of the sphingomonas sanxanigenens HL-1 has the same action effect. The microbial flocculant has advantages of small dosage, no toxicity and secondary pollution, a good flocculation effect, a wide pH range of use environment, good heat stability and the like, has a flocculation rate capable of reaching 98% when the microbial flocculant is diluted to the polysaccharide content of 4 mg / L in a flocculation system during use, and has remarkable technical economy.

Description

technical field [0001] The invention belongs to the technical field of microbial flocculants, and relates to a preparation method of an exopolysaccharide flocculant and its application in wastewater, in particular to Sphingomonas Sphingomonas sanxanigenes Application of HL-1 strain in preparation of microbial flocculant. Background technique [0002] Water is the source of life and an important condition for people to survive. However, the per capita share of water resources in our country is low, and we are still facing problems such as uncoordinated distribution, serious pollution and waste. In particular, industrial wastewater is extremely harmful to water resources. Therefore, we need effective and scientific treatment of wastewater. [0003] At present, the main technologies for treating industrial wastewater in my country include chemical oxidation, extraction, adsorption, electrodialysis and flocculation. Among them, the flocculation method is widely used because of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12P19/04C12R1/01
CPCC12N1/20C12P19/04C02F3/34
Inventor 李霜黄皓琳陶惟一余定华
Owner NANJING UNIV OF TECH
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