Compound microbial agent for straw turnover, and preparation method and application of compound microbial agent
A technology of compound microbial bacterial agent and straw, which is applied in the agricultural field, can solve the problems of not being fully utilized, and achieve the effects of reducing the accumulation of harmful organic acids, improving the fermentation effect, and inhibiting the reproduction
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Embodiment 1
[0080] A kind of composite microbial bacterial agent, its preparation method is as follows:
[0081] 1. Carry out single strain propagation of Lactobacillus pentosus, Acetobacter pasteurianus, Sphingomonas, Pichia pastoris, Hansenula cactus, Rhizopus aerizopus and Chaetomona globosa respectively:
[0082] Lactobacillus pentosus, using MRS medium, main ingredients: peptone 10g, beef extract powder 5g, yeast extract powder 4g, glucose 20g, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.2g, Manganese sulfate 0.05g, Tween 80 1g. 3% seeding, pH 6.5, 150rpm, 37°C, incubation time 20h.
[0083] Acetobacter pasteurian, using acetic acid bacteria fermentation medium, main ingredients: glucose 20g, yeast extract 5g, peptone 5g, trisodium phosphate 3g, sodium citrate 1g, water 1000ml. 5% seeding, pH 5, 30°C, 100rpm, culture time 48h.
[0084] Sphingomonas, the main components of the medium: glucose 30g / L, peptone 3g / L, yeast extract 2...
Embodiment 2
[0091] The liquid composite microbial inoculum obtained in Example 1 was mixed with rice bran, and the water was evaporated to make a solid powder to obtain a solid composite microbial inoculum.
Embodiment 3
[0093] Compared with Example 1, except that the inoculation volume ratio of Lactobacillus pentosus, Acetobacter pasteurianus and Sphingomonas is 1:1:1, other conditions are the same as Example 1.
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