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Device and method for rapid film culturing of wastewater biofilms

A film-hanging method and biofilm technology, applied in the direction of sustainable biological treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of easy shedding, long-term hanging time of wastewater biofilm, and weak firmness, etc. , to achieve the effects of good mass transfer, easy to popularize and use, and shorten the film-hanging time

Active Publication Date: 2017-02-22
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems in the prior art that the waste water biofilm hangs for a long time, is not strong, and is easy to fall off, the present invention provides a device and method for fast waste water biofilm hanging

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  • Device and method for rapid film culturing of wastewater biofilms
  • Device and method for rapid film culturing of wastewater biofilms
  • Device and method for rapid film culturing of wastewater biofilms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The activated sludge in the wastewater treatment system is difficult to adapt to the wastewater quality in a short period of time, resulting in slow system startup and unstable effluent quality. Bioaugmentation refers to the method of adding special strain formula to the biological treatment system according to plan to improve the biological treatment efficiency of the system. Bioaugmentation technology is beneficial to increase the concentration of microorganisms in the reactor, shorten the running time of the reactor, facilitate the solid-liquid separation of the reactor, enhance the impact load resistance, and ensure the start-up and stable operation of the system.

[0043] Although bioaugmentation technology has the advantages of low cost and easy maintenance, and has been successfully tested in a variety of bioreactors, but due to the problems of predictability and controllability in the bioaugmentation process have not yet been resolved, so its practical engineerin...

Embodiment 2

[0048] Such as figure 2 Shown, a kind of waste water biofilm fast film-hanging method, concrete steps are as follows:

[0049] (1), making the packing with immobilized Sphingomonas rubrum;

[0050] Step (1) The filling steps of immobilized Sphingomonas rubrum are as follows:

[0051] a. Use the pure sphingomonas rubra for expanded culture. The pure sphingomonas rubra can be obtained from the China General Microbiology Collection and Management Center. The specific preservation number is the pure sphingomonas rubraCGMCC No. 1.9113. The expanded culture conditions are: tryptone soybean broth medium with a pH of 7.0-7.2, cultured at 100-150r / min, 28°C±0.5°C shaker conditions for 24±1h;

[0052] b. Mix the bacterial solution expanded in step a with sterile waste water in a ratio of 1:100 and add it to the Erlenmeyer flask equipped with carrier filler 5, carry out bacterial agent immobilization culture, obtain the filler, and keep the OD value at 1.5±0.2 ; Culture conditions: ...

Embodiment 3

[0059] Using non-bio-augmented MBBR and bio-augmented MBBR to treat domestic sewage, compare the difference between the biofilm thickness and EPS on the filler carrier.

[0060] Table 1 shows the comparison of carrier biomass and biofilm thickness in the two devices. The two sets of devices were co-cultured for 50 days from the addition of the suspension filler. Among them, device A did not add immobilized Sphingomonas rubrum at the initial stage of MBBR reactor operation, and device B added immobilized Sphingomonas rubrum at the initial stage of MBBR reactor operation. The reactor is cylindrical, with an effective volume of 9L, a height of 45cm, and a diameter of 16cm. An aeration device is arranged at the bottom of the reactor to provide dissolved oxygen for the microorganisms and promote the circulation of the filler inside the reactor. The suspended filler in the reactor is made of polyethylene material, a hollow cylinder, the inside is a bracket support structure, and t...

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Abstract

The invention discloses a device and method for rapid film culturing of wastewater biofilms, and belongs to the technical field of wastewater treatment. For the problems of long film culturing time, weak firmness and easy falling of the wastewater biofilms in the prior art, the invention provides the device and method for the rapid film culturing of the wastewater biofilms. The device for the rapid film culturing of the wastewater biofilms comprises an MBBR, a water inlet tank is connected at the front end of the MBBR through a dosing pump, a sedimentation tank is connected at the rear end of the MBBR, an air pump supplies air for the MBBR through a pipeline, and an aerator is arranged at the bottom of the MBBR. The method for the rapid film culturing of the wastewater biofilms comprises the following steps: (1) preparing a filler with immobilized red sphingomonas; (2) carrying out aeration treatment; (3) adding the filler in the MBBR which is aerated, and adding a certain amount of the filler to an original reactor; (4) maintaining the aeration rate and a corresponding pH value until the required biofilms are obtained. By adopting the device and method for the rapid film culturing of the wastewater biofilms, the effects of the rapid film culturing speed, firm biofilms and low falling possibility can be realized.

Description

technical field [0001] The invention relates to the technical field of waste water treatment, and more specifically, relates to a device and method for rapid biofilm formation in waste water. Background technique [0002] With the continuous development of sewage biological treatment technology, biofilm method has been widely used. Among them, "moving bed biofilm reactor" is the most widely used and most efficient treatment technology in biofilm technology. It is used in domestic sewage, food wastewater, Papermaking wastewater, petroleum wastewater, pharmaceutical wastewater and other industrial wastewater have been widely used. [0003] The MBBR process is a process that combines the activated sludge method of suspended growth and the biofilm method of attached growth. This process solves the shortcoming of easy clogging of the attached growth process, has a high biomass, and does not require effluent reflux or It is sludge backflow. Therefore, the process has higher proc...

Claims

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Application Information

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IPC IPC(8): C02F3/12
CPCC02F3/12Y02W10/10
Inventor 丁丽丽傅慧敏任洪强王瑾丰朱点钰
Owner NANJING UNIV
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