Noni sphingomonas xenophaga preserving culture medium and preparation method thereof

Abstract

The invention discloses a noni sphingomonas xenophaga preserving culture medium. The culture medium is prepared from 0.8% of dry powder of nutrient broth, 0-0.5% of agar and 1-5% of a noni dried fruit aqueous solution, wherein the volume of the noni dried fruit aqueous solution is settled to be 1000ml, and the pH is adjusted to be 4.0-6.0; the preparation method of the 1-5% of noni dried fruit aqueous solution comprises the following steps of taking 10.0-50.0g of dried fruits, adding 1000ml of water, boiling over the dried fruits and the water for 10 minutes, and replenishing water to 1000ml. After the noni sphingomonas xenophaga cultured on a traditional nutrient agar culture medium is preserved for 15d, the phenomenon that the preserved noni sphingomonas xenophaga is inactive during inoculation appears. According to the preserving culture medium disclosed by the invention, the preserving time of the noni sphingomonas xenophaga can be prolonged, the longest preservation time can achieve 70d, compared with the preservation time of an ordinary nutrient agar culture medium, the preservation time of the noni sphingomonas xenophaga preserving culture medium is prolonged by 3.7 times, and the purpose of long-term preservation is achieved.

Description

technical field [0001] The invention relates to the field of preservation of microbial strains, in particular to a preservation medium for Sphingomonas noni and a preparation method thereof. Background technique [0002] Noni (Morinda officinalis) is one of the most important plants brought to Hawaii by the early Polynesians, and it is the most commonly used Chinese medicinal material by ancient Hawaiians. Noni and its related extracts play an active role in anti-tumor, improving immune regulation function, reducing liver damage and so on. Especially the use of noni fruit alone or in combination with other medicines as a remedy for many ailments such as diabetes, hypertension and cancer treatment. Scientific studies have confirmed that noni juice can prolong the lifespan of mice with Lewis lung cancer and inhibit the growth of tumor cells by stimulating the immune system. The noni juice alcohol precipitate (Nonippt) has anticancer and antibiotic activity. However, the cur...

AI Technical Summary

Problems solved by technology

However, due to the late understanding of Sphingomonas, the ecological value and economic value of this bacterium have rarely been paid attention to, and the research on it has also remained at the primary stage.