33 results about "Rhodococcus strain" patented technology

The invention discloses a rhodococcus sp. strain and application thereof, belonging to the field of environmental microbiological application. The strain is named as (Rhodococcus sp.) CM-HZX1 and is now collected with the serial number of CCTCC (China Center For Type Culture Collection) NO:M 2014329 in CCTCC on July 9th, 2014. The rhodococcus sp.CM-HZX1 has relatively strong phenol degrading capacity, namely that the degradation rate is up to 93.6% after 0.5g / L of phenol is degraded for 24 hours, and the degradation rate is up to more than 90% after 1.5g / L of phenol is degraded for 48 hours. The strain is capable of tolerating the salinity of 4% and strong in adaptability; and the strain is efficient when being used for biological treatment of phenol wastewater so as to be wide in application prospect.

The invention provides a rhodococcus ruber strain, a rhodococcus ruber bacterium preparation and an application of thalli cells and extracts of rhodococcus ruber. The strain is rhodococcus ruber (Rhodococcus ruber) FIM-12 which is preserved in the China General Microbiological Culture Collection Center on April 3, 2019, with the preservation number being CGMCC No.17525. The strain can be used forpreparing rhodococcus ruber cells and extracts of the rhodococcus ruber through microorganism fermentation: after the rhodococcus ruber (Rhodococcus ruber) FIM-12 is subjected to fermentation and culture, thalli cells are collected, the thalli cells are washed with purified water so that wet cells are obtained, the wet cells are subjected to repeated freeze-thawing combined with ultrasonic wall breaking, then extraction with anhydrous alcohol is performed, and the rhodococcus ruber extracts are obtained. The rhodococcus ruber strain can be applied to industrialized fermentation and production,the rhodococcus ruber thalli cells and the rhodococcus ruber extracts are prepared, the prepared products have the functions of increasing immunity activity and restraining effects, and the rhodococcus ruber strain can be used as a production strain for further research and development.

A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.

The invention discloses a rhodococcus strain capable of degrading metamitron and an application of the rhodococcus strain. The strain is named as (Rhodococcus sp.) MET to be preserved at China Center For Type Culture Collection (for short, CCTCC) of Wuhan University, Wuhan, China on April 7th, 2014 with the preservation number being CCTCC No.M2014116. By means of the strain, metamitron can be degraded rapidly and efficiently, and pollution of metamitron to soil and water can be prevented and treated effectively.

The invention discloses a Rhodococcus strain ZWL3NT CCTCC NO: M 208206. The preparation method of the strain includes formulation of an inorganic salt substrate, strain concentration, separation and degradation of bacteria, wherein the inorganic salt substrate contains Na2HPO4.12H2O, KH2PO4, MnSO4.H2O, FeSO4.7H2O and other components; the bacterial concentration includes: formulating the soil sample and inorganic salt substrate into a suspending liquid, inoculating the suspending liquid in a certain volume of inoculating amount onto a fresh inorganic salt medium containing 3-nitrotoluene, shaking table culturing at constant temperature until obtaining the accumulation culture object with significant degradation performance; the separation adopts a plating technique to separate and purify the culture in the LB medium. The bacterial strain provided by the invention can be used to degrade the 3-nitrotoluene with the degradation rate of 99 percent, and the preparation method is simple and easy to operate.

The invention discloses a rhodococcus corynebacterioides strain and an application thereof in printing and dyeing wastewater treatment. The rhodococcus corynebacterioides strain is named as Rhodococcus corynebacterioides ZHY1-4, and the preservation number is CGMCC No. 8172. The rhodococcus corynebacterioides strain is prepared as microbial agent; the rhodococcus corynebacterioides ZHY1-4 and the microbial agent prepared from the strain are used for treating high-salt printing and dyeing wastewater or deeply treating the printing and dyeing wastewater, the deep treatment effects on a plurality of high-salt printing and dyeing wastewater and the printing and dyeing wastewater are good, and COD (chemical oxygen demand) and ammonia nitrogen can be well removed.

The invention belongs to the field of aflatoxin degradation, and discloses a method and device for degrading aflatoxin in spina date seed. A method for detecting aflatoxin by using an immunosensor is used for detecting the spina date seed treated by chlorine dioxide by using a solution containing aflatoxin to obtain the initial concentration of aflatoxin; adding a rhodococcus PD630 strain activating solution and an aflatoxin solution into a centrifugal tube, performing uniform shaking, and performing water bath reaction at 20-60 DEG C; performing fermentation treatment at room temperature; performing microfiltration treatment on the reacted sample, and detecting the concentration of residual aflatoxin in the reacted sample; and performing single factor treatment and orthogonal experiment treatment to obtain optimal conditions for degrading an aflatoxin initial concentration solution through concentration pretreatment of the rhodococcus PD630 strain activating solution. A chemical detoxification agent is combined with microorganisms, so that the operation is simple, no harm is caused to a human body, and the degradation efficiency is high.

The invention relates to separating, culturing and application methods for a Rhodococcus erythropolis strain used for degrading aflatoxin B1 (AFB1), specifically to a separating method for the Rhodococcus erythropolis strain used for degrading AFB1, a culturing method for the Rhodococcus erythropolis strain obtained by using the separating method and a method for applying the Rhodococcus erythropolis strain in degradation of AFB1. The separating method for the strain has high specificity and high efficiency and is simple to operate; and the obtained Rhodococcus erythropolis strain has the advantages of a wide source, simple preparation, mild reaction conditions, high detoxification activity, etc.

The invention discloses the application of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution. The strain is the gram-positive bacterium Rhodococcus ruber strain Y14, and was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 21, 2021, and the deposit number is GDMCC NO: 61813. The strain has significant biodegradation ability of pyrethroid insecticides, especially the highest activity to dextrofenothrin, and the number of bacterial cells is not less than 1.0×10. 6 CFU·mL ‑1 The suspension of 50mg·L in water after 6 days of treatment ‑1 D-Cyphenothrin is completely removed, and it also has a high-efficiency bioremediation effect on polluted soil. It provides a new idea to solve the problems of excessive pyrethroid pesticide residues and environmental pollution in agricultural production, and also enriches the germplasm resource bank of pesticide-degrading bacteria, which is of great practical importance for the production of non-toxic and pollution-free green agricultural products. Value.

The invention discloses a rhodococcus strain and its screening method and application. The key points of the technical scheme of the present invention are: a strain of Rhodococcus Rhodococcus? cercidiphylli, preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC? No. 9067. The invention also discloses the screening method of the Rhodococcus syringae and its application in degrading nitrosamines. The screening method of the rhodococcus strains of the present invention is simple and fast, and can be used in drinking water polluted by nitrosamines to degrade and remove nitrosamines, and carry out screening on drinking water polluted by nitrosamines. Biological treatment to reduce its health and safety risk level.

The invention relates to a method of microbial detoxication for aflatoxin B1 (AFB1) in feed or raw materials of the feed and uses, and particularly relates to a method of using rhodococcus erythropolis for detoxication of AFB1 in feed or raw materials of the feed and uses thereof. Efficient degradation detoxication of the AFB1 in the feed or the raw materials of the feed can be achieved by uniformly mixing target AFB1-containing feed or raw materials thereof with a rhodococcus erythropolis strain fermentation broth or a liquid supernatant of the fermentation broth and reacting. The method can reduce the content of the AFB1 in the feed or the raw materials of the feed in an efficient, mild and safe manner, and is low in energy consumption, low in equipment requirement, low in environment cleanliness requirement, free of need of continuous ventilation, low in waste liquid (dreg) yield, low in environment pollution, and particularly suitable for AFB1 detoxication in the field of feed processing.

The invention discloses a rhodococcus strain and its application for degrading zearalenone. The preservation number of the Rhodococcus sp. is CGMCC No.7186. The invention also provides a biological detoxification agent containing the preserved strain and a preparation method thereof. In addition, the present invention also provides the application of the strain or biological detoxification agent in degrading zearalenone.

The present invention provides a strain of Rhodococcus ruber, a bacterial preparation and the application of its cells and extracts. The strain is Rhodococcus ruber FIM-12, which was preserved in China Microorganism Culture Collection on April 03, 2019 General Microbiology Center of the Management Committee, the preservation number is CGMCC No.17525; it can be used for microbial fermentation to prepare Rhodococcus cells and their extracts: Rhodococcus ruber (Rhodococcus ruber) FIM‑12 is fermented and cultured to collect bacterial cells and wash with purified water Wet cells are obtained; the wet cells are repeatedly frozen and thawed combined with ultrasonic breaking, and then extracted with absolute ethanol to obtain the Rhodococcus erythrococcus extract. The rhodococcus erythrococcus strain of the invention can be applied to industrial fermentation production to prepare rhodococcus erythrococcus cells and extracts of the rhodococcus erythrococcus. The prepared product has the functions of improving immune activity and tumor inhibition, and can be used as a production strain for further research and development.

The invention relates to a method for microorganism detoxifying of aflatoxin B1 (AFB1) in brewing seasoning or its raw materials and an application thereof. Specifically, the invention relates to a method for detoxifying AFB1 in brewing seasoning or its raw materials and an application thereof. By uniformly mixing the target AFB1-containing brewing seasoning or its raw materials with a rhodococcus erythropolis strain fermentation broth or its fermentation broth supernatant, ventilating and fully reacting, AFB1 in the brewing seasoning or its raw materials can be efficiently degraded and detoxified. By the method of degrading AFB1 in the brewing seasoning or its raw materials, content of AFB1 in the brewing seasoning or its raw materials can be efficiently, mildly and safely reduced. In addition, energy consumption is low, requirements for equipment are low, output of wastewater (waste residue) is low, and environmental pollution is low. The method provided by the invention is especially suitable for detoxification of AFB1 in brewing seasoning or its raw materials.

The present invention relates to a unique fermentation process of Rhodococcus ruber (CGMCC NO.17012 Rhodococcus ruber.GD0704A) and its preparation process of different formulation adjuvants and its use as an adjuvant in animal vaccines. The Rhodococcus strain is isolated from a farm and passed through The single bacterium colony is purified and identified, and the invention provides a special fermentation process when the inactivated product of the strain is used as an animal vaccine adjuvant, and provides a preparation process for adjuvants in different dosage forms containing the strain and its products. Animal experiments have confirmed that when the adjuvant products prepared by the above process are used in monovalent or multivalent animal vaccines, especially inactivated Newcastle disease vaccines, inactivated avian influenza vaccines, and live swine fever vaccines, they have clear non-specific immune enhancement The specific performance is that the peak level of antibodies induced by animal vaccines is significantly increased, the time to produce protective antibody levels is advanced, the maintenance period of antibodies is prolonged, and the immune effect of animal vaccines is enhanced.

The invention discloses a Rhodococcus ruber strain and an application thereof in treatment of wastewater containing organic pollutants. The strain is named as Rhodococcus ruber ZHY1-6, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.8173. A microbial agent is prepared from the Rhodococcus ruber strain; the Rhodococcus ruber ZHY1-6 and the microbial agent are used in a treatment technology of treating wastewater containing organic pollutants, so as to degrade the organic pollutants in wastewater; when the strain and the microbial agent are used in treatment of high-salt phenol wastewater, hydrocarbon-containing wastewater and perfume wastewater, cost of wastewater treatment is reduced, and the strain and the microbial agent have important significance in protecting ecological environment and people health.

The invention discloses a Rhodococcus strain and its application, which belongs to the field of environmental microorganism application. The strain is named as Rhodococcus sp. C M‑H Z X 1 and is now preserved in the China Center for Typical Culture Collection (C C T C C for short). The number is C C T C C N O: M 2014329, and the deposit date is July 9, 2014. Rhodococcus C M-H Z X 1 provided by the invention has a strong ability to degrade phenol, and the degradation rate reaches 93.6% after 24 hours of 0.5g / L phenol degradation, and more than 90% after 48 hours of 1.5g / L phenol degradation; the bacterial strain The invention can tolerate 4% salinity and has strong adaptability; the invention has high efficiency in the biological treatment of phenolic waste water and has broad application prospects.

The invention relates to a method for microorganism detoxifying of aflatoxin B1 (AFB1) in brewing seasoning or its raw materials and an application thereof. Specifically, the invention relates to a method for detoxifying AFB1 in brewing seasoning or its raw materials and an application thereof. By uniformly mixing the target AFB1-containing brewing seasoning or its raw materials with a rhodococcus erythropolis strain fermentation broth or its fermentation broth supernatant, ventilating and fully reacting, AFB1 in the brewing seasoning or its raw materials can be efficiently degraded and detoxified. By the method of degrading AFB1 in the brewing seasoning or its raw materials, content of AFB1 in the brewing seasoning or its raw materials can be efficiently, mildly and safely reduced. In addition, energy consumption is low, requirements for equipment are low, output of wastewater (waste residue) is low, and environmental pollution is low. The method provided by the invention is especially suitable for detoxification of AFB1 in brewing seasoning or its raw materials.