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A Rhodococcus strain and its application in printing and dyeing wastewater treatment

A printing and dyeing wastewater and rhodococcus technology, which is applied in textile industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of reduced capacity and ineffective effects, and achieve low production cost, Reduce production cost and use cost, easy to use

Active Publication Date: 2015-10-21
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since printing and dyeing wastewater is high-salt wastewater, salt-tolerant microorganisms are required to achieve good treatment effects, and most microorganisms grow in high-salt environments and their ability to metabolize organic pollutants decreases, so that the desired effect cannot be achieved. In addition, the current standard upgrading and transformation The implementation of the printing and dyeing enterprises has raised a thorny problem, and it is also very challenging

Method used

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  • A Rhodococcus strain and its application in printing and dyeing wastewater treatment
  • A Rhodococcus strain and its application in printing and dyeing wastewater treatment
  • A Rhodococcus strain and its application in printing and dyeing wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Acquisition and identification of Rhodococcus ZHY1-4

[0042] 1. Acquisition of Rhodococcus ZHY1-4

[0043] (1) Take the activated sludge for treating high-salt dye wastewater, and the medium components are as follows: yeast extract powder 0.01%, MgSO 4 0.05%, KH 2 PO 4 0.05%, Na 2 HPO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, FeCl 3 0.001%, NaCl 3%, azo dye final concentration of 0.01% (g / V), pH 7.0-7.5, sterilized at 121 °C for 20 min. After the culture medium was cooled, 10 mL of activated sludge was inoculated, and incubated at 35°C with a shaker at 130 rpm to observe the decolorization of the dye. The medium with decolorizing effect on the dye was selected and inoculated in the same medium for multiple enrichment cultures.

[0044] (2) Take the bacterial liquid that has decolorizing effect on azo dyes for gradient dilution, and use the plate coating method to spread the bacterial liquid on 3%NaCl and 4%NaCl plates, and invert at 35 °C and cultivate in a cons...

Embodiment 2

[0056] The preparation of embodiment 2 microbial inoculum and treatment of printing and dyeing wastewater

[0057] (1) Slanted seeds: The strain ZHY1-4 was activated and cultivated in a petri dish or test tube for later use.

[0058] (2) Shake flask seeds: inoculate the activated single colony in the medium (peptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, glucose 1g / L, pH 7.0-7.2), 35 ℃ Shaking culture to late logarithmic growth, ready to inoculate into seed pots.

[0059] (3) Seed tank: prepare seed tank medium (medium components are: peptone 5g / L, yeast extract powder 5g / L, NaCl 10g / L, glucose 2g / L, pH 7.0-7.5), and 30L seeds are cultured The base was added to a 50L seed tank, sterilized by high pressure moist heat at 121°C, cooled to 33°C, inoculated into the seed tank at an inoculum of 10% (V / V), and cultured at 35°C to logarithmic growth phase, stirring The speed is 180 rpm, and the sterile air flow rate is 1:0.8 (V / V).

[0060] (4) Fermentation tank: The composi...

Embodiment 3

[0070] Example 3 Treatment of high-salt dye wastewater by Rhodococcus ZHY1-4

[0071] (1) Pick a single colony of Rhodococcus ZHY1-4 and inoculate it in the medium (peptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, glucose 1g / L, pH 7.0-7.2), 35 ℃, Shake culture at 180 rpm for 48-72 hours to obtain fresh bacterial liquid.

[0072] (2) The printing and dyeing wastewater is taken from the wastewater of the adjustment tank of a printing and dyeing factory, and the salt content is about 2%.

[0073] (3) The treatment process adopts anaerobic-contact oxidation-water effluent, the reactor is a 2L cylinder, the carrier in the reactor is a polyurethane carrier, and the sludge in the anaerobic reactor is taken from the anaerobic sludge of the dye factory. Dosing at a volume ratio of 10% (V / V).

[0074] Contact the activated sludge in the oxidation tank: add 1% (V / V) of activated sludge from the urban sewage treatment plant and 10% (V / V) of Rhodococcus. During the domestication pro...

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Abstract

The invention discloses a rhodococcus corynebacterioides strain and an application thereof in printing and dyeing wastewater treatment. The rhodococcus corynebacterioides strain is named as Rhodococcus corynebacterioides ZHY1-4, and the preservation number is CGMCC No. 8172. The rhodococcus corynebacterioides strain is prepared as microbial agent; the rhodococcus corynebacterioides ZHY1-4 and the microbial agent prepared from the strain are used for treating high-salt printing and dyeing wastewater or deeply treating the printing and dyeing wastewater, the deep treatment effects on a plurality of high-salt printing and dyeing wastewater and the printing and dyeing wastewater are good, and COD (chemical oxygen demand) and ammonia nitrogen can be well removed.

Description

technical field [0001] The invention relates to the technical field of microbial treatment of printing and dyeing wastewater, in particular to a Rhodococcus, a microbial inoculum prepared from the Rhodococcus, and the application of the microbial inoculum in the treatment of printing and dyeing wastewater. Background technique [0002] In recent years, with the development of chemical fiber fabrics, the rise of simulation technology and the advancement of printing and dyeing finishing technology, a large number of organic substances that are difficult to biochemically degrade, such as PVA slurry, rayon alkaline hydrolysate, new auxiliaries, and penetrants, enter the printing and dyeing wastewater. The removal rate of COD by the original biological treatment system is reduced from 70% to about 50%, or even lower. Due to the low dyeing rate of domestic dyes, printing and dyeing enterprises generally add excessive dyes in the process. Dyes such as azo, anthraquinone, heterocycl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/30
Inventor 王慧荣梅荣武韦彦斐李明智张宇
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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