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Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment

A technology of printing and dyeing wastewater and rhodococcus, which is applied in textile industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as failure to achieve results and capacity decline, and reduce production costs and Use cost, low production cost, easy to use effect

Active Publication Date: 2014-03-26
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since printing and dyeing wastewater is high-salt wastewater, salt-tolerant microorganisms are required to achieve good treatment effects, and most microorganisms grow in high-salt environments and their ability to metabolize organic pollutants decreases, so that the desired effect cannot be achieved. In addition, the current standard upgrading and transformation The implementation of the printing and dyeing enterprises has raised a thorny problem, and it is also very challenging

Method used

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  • Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment
  • Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment
  • Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The acquisition and identification of embodiment 1 Rhodococcus ZHY1-4

[0042] 1. Obtaining Rhodococcus ZHY1-4

[0043] (1) Take the activated sludge from the treatment of high-salt dye wastewater, and the composition of the medium is as follows: yeast extract powder 0.01%, MgSO 4 0.05%, KH 2 PO 4 0.05%, Na 2 HPO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, FeCl 3 0.001%, NaCl3%, the final concentration of azo dye is 0.01% (g / V), pH7.0-7.5, sterilized at 121°C for 20min. After cooling the medium, inoculate 10mL of activated sludge, culture on a shaker at 35°C and 130 rpm, and observe the decolorization of the dye. Select the culture solution that has a decolorizing effect on the dye and inoculate it in the same medium for multiple enrichment cultures.

[0044] (2) Take the bacterial solution that has a decolorizing effect on azo dyes for gradient dilution, and use the plate coating method to spread the bacterial solution on a 3% NaCl and 4% NaCl plate, and incubate it in a co...

Embodiment 2

[0056] Preparation and treatment of printing and dyeing wastewater of embodiment 2 microbial bacterial agents

[0057] (1) Inclined seeds: activate and cultivate the strain ZHY1-4 in a petri dish or test tube for later use.

[0058] (2) Shake flask seeds: inoculate the activated single colony in the culture medium (peptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, glucose 1g / L, pH7.0-7.2), 35℃ Shake culture to late logarithmic growth, ready to inoculate into seed pots.

[0059] (3) Seed tank: prepare seed tank medium (medium composition: peptone 5g / L, yeast extract powder 5g / L, NaCl10g / L, glucose 2g / L, pH7.0-7.5), and cultivate 30L seeds The base was added to a 50L seed tank, sterilized by high-pressure damp heat at 121°C, cooled to 33°C, inoculated into the seed tank at an inoculation amount of 10% (V / V), cultivated at 35°C until the logarithmic growth phase, and stirred The speed is 180 rpm, and the sterile air intake is 1:0.8 (V / V).

[0060] (4) Fermentation tank: Th...

Embodiment 3

[0070] Example 3 Rhodococcus ZHY1-4 treatment of high-salt dye wastewater

[0071] (1) Pick a single colony of Rhodococcus ZHY1-4 and inoculate it in the culture medium (peptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, glucose 1g / L, pH7.0-7.2), 35°C, Shake culture at 180 rpm for 48-72 hours to obtain fresh bacterial liquid.

[0072] (2) The printing and dyeing wastewater is taken from the regulating pool wastewater of a printing and dyeing factory, with a salt content of about 2%.

[0073] (3) The treatment process adopts anaerobic-contact oxidation-water outlet. The reactor is a 2L cylinder. The carrier in the reactor is a polyurethane carrier. The sludge in the anaerobic reactor is taken from the anaerobic sludge of the dye factory. Dosing at a volume ratio of 10% (V / V).

[0074] Contact the activated sludge in the oxidation tank: add 1% (V / V) of activated sludge from urban sewage treatment plants and 10% (V / V) of Rhodococcus, and add 10% (V / V) of Rhodococcus per day ...

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Abstract

The invention discloses a rhodococcus corynebacterioides strain and an application thereof in printing and dyeing wastewater treatment. The rhodococcus corynebacterioides strain is named as Rhodococcus corynebacterioides ZHY1-4, and the preservation number is CGMCC No. 8172. The rhodococcus corynebacterioides strain is prepared as microbial agent; the rhodococcus corynebacterioides ZHY1-4 and the microbial agent prepared from the strain are used for treating high-salt printing and dyeing wastewater or deeply treating the printing and dyeing wastewater, the deep treatment effects on a plurality of high-salt printing and dyeing wastewater and the printing and dyeing wastewater are good, and COD (chemical oxygen demand) and ammonia nitrogen can be well removed.

Description

technical field [0001] The invention relates to the technical field of microbial treatment of printing and dyeing wastewater, in particular to a rhodococcus, a microbial agent prepared by the rhodococcus, and an application of the microbial agent in the treatment of printing and dyeing wastewater. Background technique [0002] In recent years, with the development of chemical fiber fabrics, the rise of simulation technology and the advancement of printing and dyeing finishing technology, a large number of organic substances that are difficult to biodegrade, such as PVA size, rayon hydrolyzate, new additives, and penetrants, have entered the printing and dyeing wastewater. The COD removal rate of the original biological treatment system dropped from 70% to about 50%, or even lower. Due to the low dyeing rate of domestic dyes, printing and dyeing enterprises generally add excessive dyes in the process, such as azo, anthraquinone, heterocycle, triphenylmethane and other dyes ar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/30
Inventor 王慧荣梅荣武韦彦斐李明智张宇
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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