Pseudomonas strain and identification and application methods thereof

Abstract

The invention relates to a pseudomonas strain and identification and application methods thereof in the technical field of microorganisms. The rhodococcus strain has a 16S rDNA (16S ribosome deoxyribonucleic acid) gene sequence shown as SEQ ID NO.1, a strain name of SINOPEC21 and a preservation number of CCTCC NO: M2016111 in China Center for Type Culture Collection. The abundance of the pseudomonas strain in soil above an oil reservoir is positively related to the concentration of floating gaseous hydrocarbons of the oil reservoir, so that the pseudomonas strain can serve as an oil exploitation microorganism to mark areas with high hydrocarbon leakage above the oil reservoir; meanwhile, the pseudomonas strain is high in abundance in soil above the oil reservoir and saves amplification culture, the 16S rDNA conserved sequence of the pseudomonas strain SINOPEC21 in soil through improved primers, so that the concentration of floating gaseous hydrocarbons of the oil reservoir can be determined accurately and efficiently, and the deficiency of long detecting cycle and high requirements on purity of sample culture in traditional physiological and biochemical detection can be solved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a pseudomonas and its identification method and application. Background technique [0002] The core of microbial exploration technology is the identification of oil and gas indicator microorganisms. Usually, the relative abundance of the target microorganisms studied in the exploration process is very low in the environment, so it is necessary to stimulate the rapid reproduction of hydrocarbon oxidizing bacteria in the oil and gas reservoir environment and non-oil and gas reservoir environment under the traditional specific culture conditions. Although the above method can obtain the quantity information of microorganisms, the quantity information is actually geometrically higher than the number of microorganisms in the in-situ environment, which inevitably conceals the difference in the number of microorganisms in oil and gas reservoirs and non-oil and gas reservoir...

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Problems solved by technology

[0004] At present, there are two common detection and identification methods for Pseudomonas: 1. Physiological and biochemical identification, through the isolated pure bacteria, qualitative research on the microbial physiology and biochemistry, this method has a long cycle and puts forward higher requirements on the purity of the culture ;2. After gene extraction, 16S rDNA sequencing is performed. This method still needs to obtain pure cultures for whole gene extraction, and then use general 16S rDNA primers to obtain conserved sequences for sequencing

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