Method and device for degrading aflatoxin in spina date seeds

Abstract

The invention belongs to the field of aflatoxin degradation, and discloses a method and device for degrading aflatoxin in spina date seed. A method for detecting aflatoxin by using an immunosensor is used for detecting the spina date seed treated by chlorine dioxide by using a solution containing aflatoxin to obtain the initial concentration of aflatoxin; adding a rhodococcus PD630 strain activating solution and an aflatoxin solution into a centrifugal tube, performing uniform shaking, and performing water bath reaction at 20-60 DEG C; performing fermentation treatment at room temperature; performing microfiltration treatment on the reacted sample, and detecting the concentration of residual aflatoxin in the reacted sample; and performing single factor treatment and orthogonal experiment treatment to obtain optimal conditions for degrading an aflatoxin initial concentration solution through concentration pretreatment of the rhodococcus PD630 strain activating solution. A chemical detoxification agent is combined with microorganisms, so that the operation is simple, no harm is caused to a human body, and the degradation efficiency is high.

Description

technical field [0001] The invention belongs to the field of aflatoxin degradation, and in particular relates to a method and device for degrading aflatoxin in Ziziphus jujuba. Background technique [0002] At present, the removal and degradation of aflatoxin has attracted widespread attention of researchers. Aflatoxins are potent carcinogenic compounds mainly produced by certain strains of Aspergillus. Due to their high stability under different conditions, it is very difficult to completely eliminate them from human diet and animal feed. In this way aflatoxins trigger many health problems (e.g. liver cancer). Suanzaoren, as an important traditional Chinese medicine, has the effect of nourishing the heart and nourishing the liver. But it is also very susceptible to aflatoxin, and the traditional methods to detoxify aflatoxin in jujube seed etc. include physical and chemical treatment, such as extrusion cooking process and ammoniation process. At the same time, consideri...

AI Technical Summary

Problems solved by technology

[0003] Through the above analysis, the existing problems and defects of the prior art are: the physical degradation method in the prior art may bring harm to the researchers, the research on the method of microbial degradation is not extensive, and the strain culture is complicated

[0004] The existing technology has poor effect on the degradation of aflatoxin

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