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Deep-sea actinomycete rhodococcus erythropolis and application thereof in inhibition of aflatoxin

A technology of Rhodococcus rhodochrous and actinomycetes, which can be applied in the fields of application, bacteria, edible seed preservation, etc., and can solve problems such as rare

Pending Publication Date: 2021-07-30
HARBIN INST OF TECH AT WEIHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Today, as land resources are increasingly scarce, countries around the world are turning their targets to the ocean, especially the development and utilization of microbial resources in extreme environments such as the ocean and deep sea have become the focus of world attention and competition. The development of deep-sea microbial resources plays an important role in the prevention and control of aflatoxin pollution. Research and application are rare in the world

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the fermentation of Rhodococcus erythropolis BC14-M2AF-1 bacterial strain

[0013] Inoculate the liquid seeds of Rhodococcus erythropolis BC14-M2AF-1 strain with 1-8% inoculum amount containing 0.5% sodium acetate, 0.05% peptone, 0.05% yeast extract powder, 0.05% beef extract, 0.05% glucose, sucrose 0.05%, soluble starch 0.05%, trisodium citrate 0.005%, malic acid 0.005%, potassium sodium tartrate 0.005%, ammonium nitrate 0.1%, ammonium chloride 0.02%, fresh water 100ml liquid medium, at 18-32 ℃ After culturing for 4-10 days at a temperature of 100-240 rpm, the fermentation broth is centrifuged, and the obtained cell-free supernatant is the supernatant containing active substances that efficiently inhibit the synthesis of aflatoxin, which can be directly used in Inhibit the synthesis of fungal aflatoxin, or process into powder to inhibit the synthesis of fungal aflatoxin.

Embodiment 2

[0014] Example 2: Inhibition of Fermented Cell-Free Supernatant of Rhodococcus erythropolis BC14-M2AF-1 Strain on Aflatoxin Synthesis

[0015] In the cell-free fermentation supernatant obtained in Example 1, according to the ratio of 2% glucose and 0.5% yeast extract, it was added to the fermentation supernatant to supplement nutrition, as a culture method for cultivating aflatoxin-producing bacteria-Aspergillus parasiticus liquid, the contrast culture liquid is that 2% glucose and 0.5% yeast extract are added in the liquid culture medium in embodiment 1, after inoculating the spore suspension of 1% Aspergillus parasiticus respectively in above-mentioned culture medium, cultivate 6 days at 28 degrees, The mycelium was collected by centrifugation, and the intermediate product of the aflatoxin biosynthesis pathway in the mycelia was extracted with methanol-sodium hydroxide solution, and the optical density value (OD 560 ), calculate the inhibitory rate, the inhibitory rate (%)=(...

Embodiment 3

[0016] Embodiment 3: the high-temperature and high-pressure stability of the active substance in the fermented liquid of Rhodococcus erythropolis BC14-M2AF-1

[0017] The cell-free fermentation supernatant that embodiment 1 obtains is in 121 o After C, 103kPa high temperature and high pressure treatment for 30 minutes, cool down to room temperature naturally, after inoculating the spore suspension of 1% Aspergillus parasiticus respectively with the fermented liquid of above-mentioned treatment, the fermented liquid not through temperature treatment, and control culture liquid together, in 28 Cultivate for 6 days, collect the mycelium by centrifugation, extract the intermediate product of the aflatoxin biosynthesis pathway in the mycelium with methanol-sodium hydroxide solution, and measure the optical density value (OD 560 ), calculate the inhibitory rate, the inhibitory rate (%)=(OD of the control culture solution 560 - OD of cell-free fermentation supernatant 560 ) / OD of c...

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Abstract

The invention provides a deep-sea actinomycete strain capable of inhibiting synthesis of aflatoxin. The actinomycete strain is a rhodococcus erythropolis BC14-M2AF-1 strain, is separated from sediments at water depth of 6105 meters in West Pacific Ocean, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No.61239. The BC14-M2AF-1 strain is fermented in a freshwater M2 culture medium, and the obtained fermented cell-free supernate can completely inhibit aflatoxin from the source of an aflatoxin synthesis route; and the fermentation cell-free supernate is treated at 121 DEG C and 103 kPa for 30 minutes at high temperature and high pressure, and synthesis of aflatoxin can still be inhibited by 100%. The rhodococcus erythropolis BC14-M2AF-1 strain and the fermentation product thereof can be used for effectively preventing and controlling aflatoxin pollution in field crop cultivation, grain storage, food and beverage processing, feed production and the like.

Description

Technical field: [0001] The invention relates to the technical field of deep-sea biotechnology and aflatoxin biological control, in particular to a deep-sea actinomycete Rhodococcus erythropolis and its function of inhibiting aflatoxin. Background technique: [0002] Aflatoxins are mycotoxins listed as Class I carcinogens by the International Agency for Cancer. They are mainly produced by Aspergillus flavus, Aspergillus parasiticus and other Aspergillus fungi. The resulting food and feed also have aflatoxin pollution. After humans and animals eat food and feed contaminated by aflatoxin, it can seriously endanger the health of humans and animals. Therefore, effective inhibition of aflatoxin in food and feed Pollution of food, etc. is a major food and feed safety problem that needs to be solved urgently in the world, and it has important practical significance and significant economic and social benefits. [0003] Today, as land resources are increasingly scarce, countries ar...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G13/00A01G7/06A23B9/28A23L3/3571A23L2/44C12R1/01
CPCC12N1/20A01G13/00A01G7/06A23B9/28A23L3/3571A23L2/44
Inventor 闫培生邢雪李玥昕高秀君王迪蒋文俊曹立新
Owner HARBIN INST OF TECH AT WEIHAI
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