A method for synthesizing nano-silver using Pseudomonas phlegm cell-free supernatant and its products and applications

A supernatant, cell-free technology, applied in the field of green biosynthesis of nanomaterials, can solve problems such as insignificant effects, and achieve the effects of low price, simple synthesis method and stable structure

Active Publication Date: 2021-09-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to control the disease, many compounds such as chlorine dioxide glutaraldehyde, bisalmonium chloride, cefdonium chloride, etc. have been applied in field trials, but the effect is not obvious

Method used

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  • A method for synthesizing nano-silver using Pseudomonas phlegm cell-free supernatant and its products and applications
  • A method for synthesizing nano-silver using Pseudomonas phlegm cell-free supernatant and its products and applications
  • A method for synthesizing nano-silver using Pseudomonas phlegm cell-free supernatant and its products and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0043] (2) Collection of supernatant: centrifuge the bacterial suspension obtained in step (1) at 8000 rpm for 10 minutes and collect the supernatant to obtain cell-free supernatant (CFCS);

[0044] (3) Preparation of nano silver: get the bacterial supernatant obtained in step (2) and 1mM AgNO 3 The solution was fully mixed according to the ratio of 1:3, put into a 200rpm shaker and shaken in the dark for 48h, then the obtained reaction solution was passed through 8000rpm, centrifuged for 15min, washed 3 times with distilled water, and vacuum freeze-dried to obtain nano silver particles.

Embodiment 2

[0046](1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0047] (2) centrifuge the bacterial suspension obtained in step (1) at 10000 rpm for 10 min and collect the supernatant to obtain a cell-free supernatant (CFCS);

[0048] (3) take the bacterial supernatant obtained in step (2) and 0.5mM AgNO 3 The solution was fully mixed according to the ratio of 1:5, and placed in a 200rpm shaker for 48h in the dark. Then the obtained reaction solution was subjected to 8000 rpm, centrifuged for 10 min, washed with distilled water for 3 times, and vacuum freeze-dried to obtain silver nanoparticles.

Embodiment 3

[0050] (1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0051] (2) centrifuge the bacterial suspension obtained in step (1) at 9000 rpm for 15 minutes and collect the supernatant to obtain a cell-free supernatant (CFCS);

[0052] (3) get the bacterial supernatant that step (2) obtains and 1.5mM AgNO 3 The solution was fully mixed according to the ratio of 1:4, and placed in a 200rpm shaker for 48h in the dark. Then the obtained reaction solution was subjected to 10000 rpm, centrifuged for 15 min, washed with distilled water for 3 times, and vacuum freeze-dried to obtain silver nanoparticles.

[0053] The performance and bacteriostatic effect of the nano-silver particles obtained through the mediation of Pseudomonas phlegm in Example 1...

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Abstract

The invention discloses a method for synthesizing nano-silver by using the cell-free supernatant of Pseudomonas pseudomonas. The suspension is centrifuged, and the supernatant is collected to obtain a cell-free supernatant; 3) the cell-free supernatant is added to AgNO 3 solution, the obtained reaction solution is centrifuged, washed, and vacuum freeze-dried to obtain nano silver particles. The nano-silver particles prepared by the invention have stable structure, good dispersibility, good biocompatibility, strong in vitro and in vivo antibacterial activity against Dickeya dadantii CZ1501, and can be made into eco-friendly and biocompatible nano Metal fungicides are used to prevent and control sweet potato stem rot, which reduces the burden of traditional chemical fungicides on the environment, and has a good promotion prospect in the field of agricultural production.

Description

technical field [0001] The invention belongs to the technical field of green biosynthetic nanomaterials, and specifically relates to a method for synthesizing nanosilver by utilizing the cell-free supernatant of Pseudomonas phlegm and its application in agriculture. Background technique [0002] Nanomaterials refer to their structural units in the range of 1-100nm. Because of their unique physical and chemical properties, such as small size and large specific surface area, they have broad development potential in the fields of science and technology. Among them, silver nanoparticles (AgNPs) have strong antibacterial properties and have potential antibacterial activity against Gram-negative and Gram-positive bacteria such as Escherichia coli and Staphylococcus aureus. [0003] At present, the traditional methods of synthesizing nano-silver mainly include physical methods, chemical methods, and microbial methods. The use of physical methods and chemical methods is simple and f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38
CPCA01N59/16C12P3/00
Inventor 李斌洪纤纤张牧晨杨敏邱文张阳阿弗萨娜
Owner ZHEJIANG UNIV
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