Rhodococcus strains and their applications

A technology of rhodococcus and strains, applied to bacteria, fixed on/in organic carriers, water/sludge/sewage treatment, etc., can solve the problems of low phenolic concentration tolerance, low degradation rate, and constraints, Achieve the effect of strong adaptability and broad application prospects

Active Publication Date: 2017-09-01
浙江至美环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on the above scientific and patent documents, although a variety of microorganisms screened out can degrade phenol to a certain extent, the environmental conditions for degradation are harsh, the degradation rate is low, and the concentration of phenols is low, so the promotion to practical application is greatly restricted.

Method used

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  • Rhodococcus strains and their applications
  • Rhodococcus strains and their applications
  • Rhodococcus strains and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of Rhodococcus sp. CM-HZX1

[0034] 1. Isolation of Rhodococcus sp. CM-HZX1

[0035] Rhodococcus sp. CM-HZX1 was isolated from the activated sludge in the aerobic tank of Huzhou sewage treatment plant.

[0036] (1) culture medium

[0037] Enrichment medium: yeast powder 5g, peptone 10g, NaCl 10g, add distilled water to make up to 1000mL, pH: 7.0-7.2, sterilize at 121°C for 20 minutes.

[0038] Inorganic salt medium: (NH 4 ) 2 SO 4 : 1g / L, CaCl 2 : 0.1g / L, K 2 HPO 4 : 0.5g / L, KH 2 PO 4 : 0.5g / L, MgSO 4 : 0.5g / L, NaCl: 1g / L, a certain amount of phenol, constant volume with phenol-free distilled water, pH: 7.0~7.2, sterilized at 121°C for 20 minutes.

[0039] Solid medium: add 1.5-2% agar to the above liquid medium components.

[0040] (2) Isolation and purification of bacterial strains

[0041] Take 10g of activated sludge and inoculate it into 100ml enriched medium with a phenol concentration of 100mg / L, shake it on a sha...

Embodiment 2

[0062] The best growth condition optimization of embodiment 2 Rhodococcus (Rhodococcus sp.) CM-HZX1

[0063] Medium: (NH 4 ) 2 SO 4 : 1g / L, CaCl 2 : 0.1g / L, K 2 HPO 4 : 0.5g / L, KH 2 PO 4 : 0.5g / L, MgSO 4 : 0.5g / L, NaCl: 1g / L, a certain amount of phenol, constant volume with phenol-free distilled water, pH: 7.0~7.2.

[0064] (1) Effect of the initial pH value of the medium on the degradation efficiency of phenol by Rhodococcus sp. CM-HZX1

[0065] By changing the pH value in the medium, the influence of the initial pH on the degradation efficiency of phenol by Rhodococcus sp. CM-HZX1 was investigated. Prepare 300 mL of phenol culture medium with a concentration of 500 mg / L, and the transfer volume is about 15%. Centrifuge the sludge and add it to the phenol culture medium with a pH of 3-11. For blank comparison, under the conditions of 30°C and 150r / min shaker culture, the supernatant was centrifuged for a certain period of time and the value of volatile phenol was de...

Embodiment 3

[0073] The salt resistance of the Rhodococcus strain of embodiment 3 degrading phenol

[0074] In complex industrial phenol-containing wastewater, the salt content is relatively high. By changing the salt content in the medium, the salt-resistant ability of Rhodococcus strains that degrade phenol was investigated. Prepare a phenol culture medium with a concentration of about 500mg / L, and transfer the volume to about 15%. Centrifuge the sludge and add it to the phenol culture medium. The initial pH value is 7. Cultivate in a shaker at 30°C and 150r / min, with a certain period of time. , and centrifuged to get the supernatant to detect the value of volatile phenol by direct spectrophotometry with 4-aminoantipyrine.

[0075] Depend on Image 6 It can be seen that when the salt content of the strain of the present invention is 0.5-4%, the phenol degradation rate is about 90%, indicating that the microorganism belongs to moderate salt-tolerant microorganism.

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Abstract

The invention discloses a Rhodococcus strain and its application, which belongs to the field of environmental microorganism application. The strain is named as Rhodococcus sp. C M‑H Z X 1 and is now preserved in the China Center for Typical Culture Collection (C C T C C for short). The number is C C T C C N O: M 2014329, and the deposit date is July 9, 2014. Rhodococcus C M-H Z X 1 provided by the invention has a strong ability to degrade phenol, and the degradation rate reaches 93.6% after 24 hours of 0.5g / L phenol degradation, and more than 90% after 48 hours of 1.5g / L phenol degradation; the bacterial strain The invention can tolerate 4% salinity and has strong adaptability; the invention has high efficiency in the biological treatment of phenolic waste water and has broad application prospects.

Description

technical field [0001] The invention relates to the application field of environmental microorganisms, in particular to a rhodococcus strain and its application. Background technique [0002] Phenol-containing wastewater mainly comes from oil refining, coking, gas, and chemical, papermaking, smelting, textile printing and dyeing production processes that use phenol or phenolic formaldehyde as raw materials. This kind of wastewater has a wide range of sources, large water volume, complex components, and high toxicity. In water pollution control, it is listed as one of the key toxic and harmful wastewater to be solved. [0003] At present, the treatment methods of phenolic wastewater mainly include physical, chemical and biological methods. The physical methods are adsorption and extraction methods, but the selectivity is poor and it is easy to cause secondary pollution. Chemical methods mainly include chemical oxidation and chemical precipitation, but the treatment costs ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/08C12N11/04C02F3/34C12R1/01C02F101/34
CPCC02F3/34C02F2101/345C12N1/20C12N11/04C12N11/08C12N11/10C12N1/205C12R2001/01
Inventor 魏霞谢柳
Owner 浙江至美环境科技有限公司
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