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A kind of preparation method of rhodococcus producing surfactant

A technology for producing surfactants and rhodococcus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of no large-scale production of biosurfactants, and achieve easy application and promotion, simple process, The effect of good emulsification

Active Publication Date: 2018-12-28
TIANJIN SEA WATER DESALINATION & COMPLEX UTILIZATION INST STATE OCEANOGRAPHI
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  • Description
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  • Application Information

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Problems solved by technology

[0004] However, in my country, biosurfactants have not yet been mass-produced, and there is room for research and development.

Method used

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  • A kind of preparation method of rhodococcus producing surfactant

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Embodiment 1

[0026] Embodiment 1, preparation fermentation medium

[0027] The fermentation medium is an inorganic salt medium.

[0028] Prepare 60L of liquid fermentation medium: 150g of urea, 60g of K2HPO4, 45g of KH2PO4, 30g of MgSO4·7H2O, add tap water to make up to 60L, adjust the pH to 7.0, add 1200g of soybean oil, and sterilize at 121°C for 30min.

[0029] Prepare 100 mL of solid fermentation medium: 0.25 g of urea, 0.1 g of K2HPO4, 0.075 g of KH2PO4, 0.05 g of MgSO4 7H2O, add distilled water to 100 mL, adjust the pH to 7.0, add 2 g of soybean oil, and sterilize at 121 ° C for 30 min.

Embodiment 2

[0030] Embodiment 2, strain fermentation culture

[0031] Inoculate a single colony of Rhodococcus on the slant of the solid medium and culture it in a 30°C incubator for 1 day, then pick a single colony with an inoculation needle and inoculate it in 500 mL of liquid fermentation medium, and culture it for 1 day at 30°C and a shaker speed of 160 rpm. Obtain the seed liquid; inoculate all the seed liquid in 50L liquid fermentation medium, cultivate 24h under the conditions of 30°C, stirring speed 160rpm, ventilation rate 30slpm (guarantee dissolved oxygen 30%), and obtain the fermentation culture liquid.

Embodiment 3

[0032] Embodiment 3, preparation biosurfactant finished product

[0033] After the fermentation stopped, the tank was removed, and the pH value of the fermentation medium was adjusted to 2 with HCl (5N), and it was allowed to stand at room temperature for 6 h; Remove excess water; extract the paste with chloroform / methanol (1:1) solution, and dry it by rotary evaporation at 70°C and 80rpm to obtain 1.128kg of finished biosurfactant.

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Abstract

The invention discloses a preparation method for a surfactant produced from rhodococcus; the rhodococcus isolated and screened from the mud of the coastal beach is utilized, and is fermented to produce the surfactant; the technical steps comprise strain activation, strain seed liquid culture, strain fermentation culture and biosurfactant preparation; rhodococcus strain is firstly inoculated into solid fermentation medium for activating, and then is inoculated into liquid fermentation medium to obtain seed liquor; the seed liquor is inoculated into the liquid fermentation medium to obtain fermentation culture liquid, and then acid precipitation, centrifugal separation, organic solvent extraction and rotary evaporation are performed on the fermentation culture liquid; a biosurfactant finished product with high surface activity is prepared. The preparation method for the surfactant produced from the rhodococcus has a simple preparation technology, good emulsification on petroleum, good market application potential, and is convenient for popularization and application.

Description

technical field [0001] The invention relates to a preparation method of a biosurfactant, in particular to a preparation method of a rhodococcus-produced surfactant. Background technique [0002] Biosurfactant is a kind of surfactant, which is produced by microorganisms and is an amphiphilic biomacromolecular substance containing both hydrophilic groups and hydrophobic groups. Compared with chemically synthesized surfactants, in addition to reducing surface tension, stabilizing emulsions and increasing foam, biosurfactants also have the following characteristics: (1) high surface and interface activity, (2) biodegradability , (3) low toxicity, (4) easy availability of raw materials, (5) economical production, (6) structural specificity. So far, hundreds of strains have been reported that can produce biosurfactants, belonging to many genera such as fungi, yeast, and bacteria. It has various sources and can produce a variety of biosurfactants. [0003] Biosurfactants have gr...

Claims

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Application Information

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IPC IPC(8): C12P1/00C12R1/01
Inventor 郝建安张雨山王静张晓青姜天翔杜瑾杨波司晓光张爱君邱金泉
Owner TIANJIN SEA WATER DESALINATION & COMPLEX UTILIZATION INST STATE OCEANOGRAPHI
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