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Application of Rhodococcus erythrococcus in degrading aflatoxin b1 in feed or its raw materials

A technology of Rhodococcus erythrococcus and aflatoxin, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of complex degradation operation process, difficulty in large-scale implementation, and low efficiency of toxin degradation, and achieve detoxification activity , less environmental pollution, and less demanding environmental cleanliness

Active Publication Date: 2019-10-15
COFCO NUTRITION & HEALTH RES INST +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are not many domestic related studies, mainly including reports by Liu Daling et al. (Research on the degradation of aflatoxin by fungal extracts [J]. Journal of Guangdong Pharmaceutical University, 1995, 11(2):92~94) found a fungal metabolite Has the function of degrading toxins; Ji Cheng et al. reported (Research on the detoxification, antibacterial and stress resistance of Bacillus subtilis degrading aflatoxin, Feed Industry, 2011, 32(24):23~27) found that a strain of Bacillus has the ability to degrade Toxin action; and Liu Yang et al. reported (screening of high-yielding laccase oyster mushroom and its use in degrading aflatoxin B1, Acta Nuclear Agricultural Sciences, 2012, 26(7):1025-1030) found that a strain of oyster mushroom has the ability to degrade Toxic effects, etc.
[0006] However, studies on the detoxification of these bacteria and fungi have found that the toxin degradation efficiency of most bacteria is not high, usually less than 50%.
In addition, there are many factors affecting the degradation efficiency, including action time, solution pH value, solution composition, bacterial count, toxin concentration and metal ions, etc. At the same time, the degradation operation process is complicated and difficult to implement on a large scale

Method used

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  • Application of Rhodococcus erythrococcus in degrading aflatoxin b1 in feed or its raw materials
  • Application of Rhodococcus erythrococcus in degrading aflatoxin b1 in feed or its raw materials
  • Application of Rhodococcus erythrococcus in degrading aflatoxin b1 in feed or its raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of embodiment 1 Rhodococcus erythrococcus strain fermented liquid and supernatant thereof

[0042] Seven strains of Rhodococcus erythropolis NHRI-1, NHRI-2, NHRI-3, NHRI-4, NHRI-5, NHRI-6 and NHRI-7 preserved in glycerol tubes at -80℃ General Microorganism Center (CGMCC), deposit numbers are CGMCC No.8590, CGMCC No.8591, CGMCC No.8592, CGMCC No.8593, CGMCC No.8594, CGMCC No.8595 and CGMCC No.8596) and purchased Add 50 μL of frozen storage solution of two strains of Rhodococcus erythrococcus CGMCC4.1491 and CGMCC1.2362 from the General Microbiology Center of China Microbiological Culture Collection Management Committee to 50mL liquid medium (composition: peptone 1.00%, beef extract 0.50%, glucose 0.50%, sodium chloride 0.50%; pH7.2-7.4, 121°C autoclave for 20min) activation culture, the culture conditions are: temperature 30°C, rotation speed 100r / min, culture time 48h. After confirming the purity of the corresponding activated culture solution by microscopi...

Embodiment 2

[0044] The degradation test of the Rhodococcus rhodococcus strain fermented liquid of embodiment 2 mixing ratio 3% to the AFB1 in the feed raw material

[0045] The fermentation broth of the strain preserved in Example 1 was evenly mixed into the feed material (corn, soybean, etc. cake mixture) polluted by AFB1 in a ratio of 3% (volume / weight ratio) (AFB1 content was 1mg / kg), each Prepare 3 mixed test samples from the fermentation broth of the strain, each sample 1000g, use sterile water to adjust the humidity to 60%-80%, put it in a 5L sterile Erlenmeyer flask, and react at a temperature of 30°C for 72h. During this process Keep the pH at 7.2-7.4. At the same time, under the same conditions, a 3% mixed sample of fermentation medium without Rhodococcus rhodococcus was set as a control.

[0046] After the reaction was completed, 5g of each sample was sampled, and the kit (MycoSep226Afazon + MultifunctionalColumns, Romer Labs Inc.MO, USA, production batch number: 226903-1306) ...

Embodiment 3

[0051] The degradation test of the Rhodococcus erythrococcus strain fermented liquid of embodiment 3 mixing ratio 8% to the AFB1 in the feed raw material

[0052] The fermentation broth of the strain preserved in Example 1 was evenly mixed into the feed material (corn, soybean, etc. cake mixture) polluted by AFB1 in a ratio of 8% (volume / weight ratio) (AFB1 content was 1mg / kg), each Prepare 3 mixed test samples from the fermentation broth of the strain, each sample 1000g, use sterile water to adjust the humidity to 60%-80%, put it in a 5L sterile Erlenmeyer flask, and react at a temperature of 30°C for 72h. During this process Keep the pH at 7.2-7.4. At the same time, under the same conditions, an 8% mixed sample of fermentation medium without Rhodococcus rhodococcus was set as a control.

[0053] After the reaction was completed, 5g of each sample was sampled, and the kit ((MycoSep226Afazon + Multifunctional Columns, Romer Labs Inc.MO, USA, production batch number: 226903-1...

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Abstract

The invention relates to a method of microbial detoxication for aflatoxin B1 (AFB1) in feed or raw materials of the feed and uses, and particularly relates to a method of using rhodococcus erythropolis for detoxication of AFB1 in feed or raw materials of the feed and uses thereof. Efficient degradation detoxication of the AFB1 in the feed or the raw materials of the feed can be achieved by uniformly mixing target AFB1-containing feed or raw materials thereof with a rhodococcus erythropolis strain fermentation broth or a liquid supernatant of the fermentation broth and reacting. The method can reduce the content of the AFB1 in the feed or the raw materials of the feed in an efficient, mild and safe manner, and is low in energy consumption, low in equipment requirement, low in environment cleanliness requirement, free of need of continuous ventilation, low in waste liquid (dreg) yield, low in environment pollution, and particularly suitable for AFB1 detoxication in the field of feed processing.

Description

technical field [0001] The present invention relates to a method and application of microbial detoxification of aflatoxin B1 (AFB1) in feed or its raw materials. A method and application of microbial degradation and detoxification of AFB1 in feed or its raw materials. Background technique [0002] Mycotoxins are secondary metabolites produced during the growth of molds. Mycotoxin contamination has always been a major threat to the global food, feed and agricultural products industries, bringing huge economic losses to production every year. Aflatoxin is one of the most toxic mycotoxins, and there are many types. AFB1, AFB2, AFG1, AFG2, AFM1, AFM2, AFB2a, AFG2a, AFBM2a and AFGM2a have been isolated as many as 18 species, and among them AFB1 has the strongest toxicity and the most stable chemical properties. It has strong carcinogenicity, mutagenicity and teratogenicity, and is an internationally recognized class A carcinogen. [0003] Aflatoxin widely exists in the prepara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01A23L5/20
Inventor 蔡军刘佳佳欧静堃李慧黄蔚霞倪媛媛刘洋
Owner COFCO NUTRITION & HEALTH RES INST
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