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Rhodococcus strain capable of degrading metamitron and application of rhodococcus strain

A technology of rhodococcus and strains, applied in the field of environmental microorganisms, to achieve the effect of pollution prevention and efficient degradation

Inactive Publication Date: 2015-08-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are few reports on the treatment and degradation of triazone-based selective pre-emergent herbicides in soil and water. Therefore, it is necessary to screen a bacterial strain that can degrade and process triazone-based herbicides

Method used

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  • Rhodococcus strain capable of degrading metamitron and application of rhodococcus strain
  • Rhodococcus strain capable of degrading metamitron and application of rhodococcus strain
  • Rhodococcus strain capable of degrading metamitron and application of rhodococcus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and Identification of Strains

[0028] 1. Isolation and purification of strains

[0029] (1) Take 3 parts of activated sludge from the wastewater aeration tank of the pesticide production plant of Anhui Zhongshan Chemical Co., Ltd., weigh 5g each and place them in a 100mL Erlenmeyer flask, add 50mL of inorganic salt medium and 5mg of fenmetrione, and place in Shake culture in a shaker (30°C, 150rpm), add 5 mg promethazone every 5 days, and culture 3 times under the same conditions;

[0030] (2) Inoculate 0.5 mL of the culture solution into 50 mL of inorganic salt medium containing 50 mg / L fenmetrione and 1 / 10 LB, place in a shaker for shaking culture (30°C, 150 rpm) for 5 days. For each culture, 0.5 mL of the previous culture solution was taken as the inoculum, and the concentration of methacmezone was increased to 100 mg / L, 200 mg / L, and 400 mg / L in turn, and the concentration of LB was decreased to 1 / 20, 1 / 100, 1 / 500;

[0031] (3) Dilute the cu...

Embodiment 2

[0042] The preparation of embodiment 2 bacterial agents

[0043] The isolate MET was inoculated in 50 mL of LB medium containing 100 mg / L fenmetrione, cultured with shaking at 30 °C and 150 rpm for 36 h, centrifuged (6500 × g) for 10 min to collect the bacteria, and then washed with 3 × 50 mL NaH 2 PO 4 -Na 2 HPO 4(0.1mol / L, pH 7.0) buffer solution to rinse the bacteria three times, and finally suspend in 20mL of the same buffer solution to make a bacterial solution for later use. The fungicide can be used to prevent and control promethazone in soil and water.

Embodiment 3

[0044] Example 3 Degradation of Memetrazone by Bacterial Strains under Different Conditions

[0045] 1. Different concentrations of fenmetrione

[0046] Add 20mL of inorganic salt medium with pH 7.0 to a 100mL sterilized Erlenmeyer flask, the concentration of methacridone is 1.0, 10.0, 100.0mg / L respectively, and the inoculum concentration OD 610 =0.1, cultured with shaking in the dark at 20°C and 150rpm.

[0047] 2. pH value

[0048] Add 20mL of inorganic salt medium prepared by pH5.0, 7.0, and 9.0 buffer solution to 100mL sterile Erlenmeyer flask respectively, add fenmetrione to a concentration of 10mg / L, and the inoculation concentration of bacteria solution is OD 610 =0.1, cultured with shaking in the dark at 20°C and 150rpm.

[0049] 3. Temperature

[0050] Add 20mL of inorganic salt medium with pH 7.0 to a 100mL sterilized Erlenmeyer flask, the concentration of fenmetrione is 10mg / L, and the inoculation concentration of the bacteria solution is OD 610 =0.1, cultured...

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Abstract

The invention discloses a rhodococcus strain capable of degrading metamitron and an application of the rhodococcus strain. The strain is named as (Rhodococcus sp.) MET to be preserved at China Center For Type Culture Collection (for short, CCTCC) of Wuhan University, Wuhan, China on April 7th, 2014 with the preservation number being CCTCC No.M2014116. By means of the strain, metamitron can be degraded rapidly and efficiently, and pollution of metamitron to soil and water can be prevented and treated effectively.

Description

technical field [0001] The invention relates to the field of environmental microbes, in particular to a rhodococcus strain for degrading fenmetrime and its application. Background technique [0002] Promethazinone, chemical name is 4-amino-4,5-dihydro-3-methyl-6-phenyl-1,2,4-triazin-5-one, molecular formula is C 10 h 10 N 4 O, the molecular weight is 202.2. The appearance of fenmetrione is light yellow to white crystalline solid; melting point is 166°C; vapor pressure (20°C): 86nPa; solubility (25°C): 1.7g / L in water, 10-50g / kg in cyclohexanone , 20-50g / L in dichloromethane, <100mg / L in hexane, 5-10g / L in isopropanol, 2-5g / L in toluene; mainly used in the preparation of herbicides. [0003] Promethazinone is a triazinone selective pre-emergent herbicide, which is absorbed by plant roots, transduced by xylem and transported to leaves. By binding to the QB binding site and receiving the electrons transferred by QA at the same time, it prevents the transfer of electrons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 虞云龙许天衡程龙吟
Owner ZHEJIANG UNIV
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