38 results about "Providencia rettgeri" patented technology

The invention discloses an ammonia nitrogen-removed compound microorganism microbial agent. The ammonia nitrogen-removed compound microorganism microbial agent is prepared from Providencia rettgeri, Microbacterium lactium, Lactobacillus delbrueckii and Rhodococcus coprophilus in a volume ratio of 5 to 2.5 to 1 to 5. The invention further discloses application of the compound microorganism microbial agent in ammonia nitrogen-containing wastewater treatment. The four bacteria are reasonably matched and are combined by virtue of an adsorption method and an embedding method to form immobilized cell particles, so that the ammonia-nitrogen degradation capacity and stability of the compound microorganism microbial agent are remarkably enhanced; a dominant bacterial community can be immediately formed after the compound microorganism microbial agent is applied to denitrification and treatment process of sewage, river way landscape water and the like, the treatment time is short, and ammonia and nitrogen indexes of the sewage are rapidly decreased; and meanwhile, the compound microorganism microbial agent has an aerobic denitrification function and further has relatively good effect of removing COD, and accumulation of nitrite and nitrate is avoided.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

The invention relates to a strain of Providencia rettgeri L1 capable of efficiently degrading petroleum and an application thereof. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2018, an address is Institute of Microbiology, Chinese Academic Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and a strain preservationnumber is CGMCC No.16526. According to the invention, the strain of Providenia has a petroleum hydrocarbon degradation function, has differential selectivity on degradation of four components in petroleum, especially has a very remarkable degradation effect on n-alkanes of one of the petroleum components, can be applied to treatment of petroleum pollutants, and has a wide application prospect.

The invention discloses a strain Pr-nj01 having insecticidal activity, an insecticidal agent and application thereof. The strain Pr-nj01 is identified as Providencia rettgeri, is preserved in the China center for type culture collection on May 10, 2017 and has a preservation number of CCTCC NO: M2017251. The separated and screened strain Pr-nj01 having insecticidal activity can be used for preventing and treating pests in fields, has broad-spectrum killing effects on pests in fields, can kill lepidoptera and hemiptera pests in fields and has good killing effects. The insecticidal agent is prepared through simple processes and has the advantages of low production cost, use convenience and good insecticidal effects. Through use of the insecticidal agent, chemical pesticide is reduced. The strain Pr-nj01 has the important significance for protecting the ecological environment and ensuring food safety.

The invention provides a degrading bacterium capable of eliminating plant fungicide residues and an application of the degrading bacterium. The degrading bacterium is identified to be providencia rettgeri, belongs to gram-negative bacteria, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 15, 2017; the preservation number is CGMCC NO. 14163. The degrading bacterium can grow with plant fungicide, bismerthiazol, as the only carbon source and energy, and the degrading rate for bismerthiazol with concentration of 100 mg / L in 96 h reaches 90% or higher; after direct application of the degrading bacterium, residues of field bismerthiazol can be significantly reduced, pollution can be effectively eliminated, the problem of over limits of the bismerthiazol residues in agricultural production is solved, and non-toxic and pollution-free green agricultural products are produced.

The invention discloses Providencia rettgeri with aniline degradation and denitrification capabilities and an application of the Providencia rettgeri. The strain is named as Providencia rettgeri Y15-7, is preserved in the CCTCC on August 26, 2019, and has a preservation number of CCTCC NO: M 2019664. The Providencia rettgeri Y15-7 can be used for relatively rapidly degrading aniline under anoxic and aerobic conditions, and a part of TN can be converted into gaseous nitrogen; and the aniline can be degraded and denitrified in the presence of a second carbon source such as glucose or sucrose andthe like, a yeast extract, peptone and other organic nitrogen under a high salinity condition, and the Providencia rettgeri has broad application prospects in actual wastewater treatment with complexwater quality.

The invention discloses an application of sanguinarine in inhibition and removal of a multi-drug-resistant providencia rettgeri biofilm and aims at solving the technical problems of drug resistance ofthe drug-resistant providencia rettgeri biofilm and difficulty in removal once the drug-resistant providencia rettgeri biofilm is formed. According to the fact that sanguinarine has a good providencia rettgeri biofilm inhibiting and removing effect, formation of the providencia rettgeri biofilm can be inhibited, and the lowest inhibition concentration of the biofilm is 7.81 micrograms per milliliter. The sanguinarine can be used for effectively removing a mature providencia rettgeri biofilm, and has the lowest biofilm removal concentration of 125 micrograms per milliliter. According to the invention, a natural product sanguinarine is adopted to inhibit and remove a multi-drug-resistant providencia rettgeri biofilm, can effectively relieve or solve the infection treatment difficulty causedby the providencia rettgeri biofilm, improves the cure rate, provides a new lead compound for inhibiting the formation of the providencia rettgeri biofilm and removing the mature biofilm, and has important practical significance.

The present invention relates to a strain of Providencia rettgeri (Providencia rettgeri) L1 that efficiently degrades petroleum and its application. The strain was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on September 26, 2018, address : Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, strain collection number CGMCC No.16526. The present invention discovers for the first time that the bacterial strain of Providencia (Providencia) has the function of degrading petroleum hydrocarbons, and has differential selectivity to the degradation of four components in petroleum, especially for normal alkanes, one of the petroleum components. It has a very significant degradation effect, can be applied to the treatment of petroleum pollutants, and has broad application prospects.

The invention discloses a strain of Providencia rettgeri with the ability to degrade aniline and denitrify and its application. The bacterium is named Providencia rettgeri (Providencia rettgeri) Y15‑7 and was released on August 26, 2019. It was deposited in the China Center for Type Culture Collection, and the deposit number is CCTCC NO: M 2019664. The Providencia rettgeri Y15-7 can degrade aniline quickly under anoxic and aerobic conditions, and can convert part of TN into gaseous nitrogen, and can be used in second carbon sources such as glucose or sucrose and In the presence of organic nitrogen such as yeast extract and peptone, and under high salinity conditions, the degradation and denitrification of aniline has broad application prospects in the actual wastewater treatment with complex water quality.

The invention discloses the application of sanguinarine in the inhibition and removal of multi-drug-resistant Providence recei biofilms, and is used to solve the drug resistance of drug-resistant Providence recei biofilms and the formation of biofilms that are difficult to remove once formed. technical problem. According to the fact that sanguinarine has good biofilm inhibitory and scavenging effects on Providencia revei, it can inhibit the formation of biofilm of Providencia revei, and the minimum inhibitory concentration of biofilm is 7.81 μg / mL. Sanguinarine can effectively remove mature Providencia reinhardtii biofilm, and the minimum biofilm removal concentration is 125μg / mL. It uses the natural product sanguinarine to inhibit and clear the biofilm of the multi-drug-resistant Providencia raptorii, which can effectively alleviate or solve the difficulty in the treatment of infections caused by the biofilm of Providencia reinhardtii, and improve the cure rate. Inhibiting the formation of Providencia reinhardtii biofilms and clearing mature biofilms provides new lead compounds with important practical significance.

The invention discloses the application of luteolin in inhibiting the growth of multi-drug-resistant Providencia rettgeri. According to the fact that luteolin has a good in vitro killing effect on multi-drug-resistant Providencia rettgeri, it can inhibit multiple The growth of drug-resistant Providencia rettgeri, and the minimum bactericidal concentration is 30 ~ 250 μg / mL, the minimum inhibitory concentration is 15 ~ 125 μg / mL, the present invention proposes that luteolin is effective against multidrug-resistant Providencia rettgeri The inhibitory effect of S. spp. has wide application value in medicine and other fields.

The invention discloses a root-knot nematode biocontrol bacterium, a biocontrol bacterium agent and applications thereof. The biocontrol bacterium is Providencia rettgeri (Providencia rettgeri) strain UN06, and the preservation number is CGMCC No.10246. The present invention utilizes the fermented liquid obtained by the fermentation of the Providencia rettgeri UN06 strain to mix evenly with adsorption carriers such as bacterial residue and peat and urea, a nutrient substance required for bacterial growth, and then granulate to prepare the UN06 granule. The control effects of applying 100 kg of UN06 granules per mu on tomato root-knot nematode, cucumber root-knot nematode and tobacco root-knot nematode were 90.17%, 88.66% and 85.98%, respectively. The root-knot nematode biological control bacterial agent of the invention has the characteristics of low cost, high control effect, no residue, and safety to humans and animals, and is a high-efficiency biological pesticide for preventing and treating root-knot nematode.

The invention discloses a bacterial strain Pr-nj01 with insecticidal activity and its production and application of insecticides. The bacterial strain Pr-nj01 has been identified as Providencia rettgeri (Providencia rettgeri) and has been preserved in the Chinese Typical Culture Collection Center, the preservation time is May 10, 2017, and the preservation number is CCTCC NO: M2017251. The present invention obtains the bacterial strain Pr-nj01 with insecticidal activity through separation and screening, which can be used for the control of field pests. The bacterial strain has a broad-spectrum killing effect on field pests, and can kill field pests such as Lepidoptera and Hemiptera, and Good killing effect. The insecticide fungicide of the present invention is simple to prepare, has the advantages of low production cost, convenient use and good insecticidal effect. The use of the fungicide can reduce the use of chemical pesticides, which is of great significance for protecting the ecological environment and ensuring food safety.

The invention provides a degrading bacterium for eliminating plant fungicide residues and application thereof, the degrading bacterium is identified as Providencia rettgeri Providencia retgeri , belonging to Gram-negative bacteria, which was deposited in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on May 15, 2017, and the preservation number is: CGMCC NO.14163. As the only carbon source and energy source for growth, the degradation rate of 100 mg / L eubacazole within 96 hours can reach more than 90%. The direct application of degrading bacteria can significantly reduce the field phylloxazole residue, which can effectively eliminate pollution and solve the problem of It solves the problem of excessive phyllazole residues in agricultural production, and produces green agricultural products that are non-toxic and pollution-free.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

The invention discloses a Providencia rettgeri strain and application thereof. The Providencia rettgeri YL is collected in China General Microbiological Culture Collection Center (CGMCC) on April 28th, 2013, and the collection number is CGMCC No.7544. The Providencia rettgeri is used for treating nitrogenous organic wastewater, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen while removing organic carbon, and thus, the whole organic carbon and nitrogen synchronous removing process can be implemented only by one aerobic stage. The strain has the heterotrophic nitrification-aerobic denitrification function, can synchronously remove the organic carbon and nitrogen under aerobic conditions, can greatly reduce the occupied area and construction investment, can shorten the system start-up time, reduces the treatment cost, and enhances the treatment efficiency.