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A strain pr-nj01 with insecticidal activity and its production and application of insecticide

A technology of pr-nj01 and insecticidal activity, applied in the direction of biocides, insecticides, applications, etc., can solve the problems of not having broad spectrum, general effect, and small range of action, so as to ensure food safety, reduce use, The effect of low production cost

Active Publication Date: 2019-09-03
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the prevention and control of agricultural pests. Patent KR20120089522A and patent CN 104663724 A disclose that Providencia bacteria can control Lepidoptera field pests, but the scope of action is small, and it can only kill diamondback moth, beet armyworm, etc. Lepidoptera field pests, and other types of field pests, such as Hemiptera, cannot be killed, do not have a broad spectrum, and the effect is general

Method used

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  • A strain pr-nj01 with insecticidal activity and its production and application of insecticide
  • A strain pr-nj01 with insecticidal activity and its production and application of insecticide
  • A strain pr-nj01 with insecticidal activity and its production and application of insecticide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and identification of strain Pr-nj01:

[0043] Some of the reared C. borer test insects died after being infected by pathogenic microorganisms. The corpses of the dead test insects were collected, sterilized with 75% ethanol by volume, and then ground with sterilized grinding rods. After centrifugation, the grinding solution was diluted with sterilized water, and then the diluted grinding solution was spread on the solid LB medium. After culturing at 30°C for 2 days, a single colony was picked and inoculated in the LB-containing liquid medium for 30 °C, 200rpm culture for 2 days to obtain the bacterial strain of the strain. The insecticidal activity of Spodoptera exigua was tested indoors with the bacterial agent, the results showed that the agent could kill the larvae of Spodoptera exigua, and a strain with insecticidal activity was obtained, which was named Pr-nj01.

[0044] After the strain Pr-nj01 was cultured on LB solid medium for 2-3 days, the colonies...

Embodiment 2

[0047] The bacterial agent preparation of above-mentioned bacterial strain Pr-nj01:

[0048] 1) Prepare LB liquid culture medium and pack in Erlenmeyer flasks, and sterilize for subsequent use;

[0049]2) Under aseptic operation conditions, inoculate the strain Pr-nj01 into the above-mentioned sterilized LB Erlenmeyer flask with an inoculation needle, shake and cultivate in a shaking table, the rotation speed is 200rpm, the cultivation temperature is 28°C, and the cultivation time is 48 hours , thus obtaining the insecticide with insecticidal activity prepared by strain Pr-nj01.

Embodiment 3

[0051] 1) Prepare LB liquid culture medium and pack in Erlenmeyer flasks, and sterilize for subsequent use;

[0052] 2) Under aseptic operation conditions, inoculate the strain Pr-nj01 into the above-mentioned sterilized LB Erlenmeyer flask with an inoculation needle, shake and cultivate in a shaker, the rotation speed is 250rpm, the cultivation temperature is 37°C, and the cultivation time is 12 hours , thus obtaining the insecticide with insecticidal activity prepared by strain Pr-nj01.

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Abstract

The invention discloses a bacterial strain Pr-nj01 with insecticidal activity and its production and application of insecticides. The bacterial strain Pr-nj01 has been identified as Providencia rettgeri (Providencia rettgeri) and has been preserved in the Chinese Typical Culture Collection Center, the preservation time is May 10, 2017, and the preservation number is CCTCC NO: M2017251. The present invention obtains the bacterial strain Pr-nj01 with insecticidal activity through separation and screening, which can be used for the control of field pests. The bacterial strain has a broad-spectrum killing effect on field pests, and can kill field pests such as Lepidoptera and Hemiptera, and Good killing effect. The insecticide fungicide of the present invention is simple to prepare, has the advantages of low production cost, convenient use and good insecticidal effect. The use of the fungicide can reduce the use of chemical pesticides, which is of great significance for protecting the ecological environment and ensuring food safety.

Description

technical field [0001] The invention relates to biological control of agricultural pests, in particular to a bacterial strain Pr-nj01 with insecticidal activity and its production and application of insecticidal fungicides. Background technique [0002] The extensive use of chemical pesticides in agricultural production, on the one hand, causes damage to the ecological environment, and on the other hand, pesticide residues affect food safety. In order to reduce the use of chemical pesticides, the development of biological pesticides has received more and more attention. Compared with traditional chemical pesticides, biological pesticides have many advantages. For example, bio-pesticides have little impact on the ecological environment; bio-pesticides have high selectivity and high safety for humans and animals; bio-pesticides are not easy to cause pest resistance and so on. [0003] At present, some biological pesticides have been used in agricultural production for biolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P7/04C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 罗光华姚静张茹杨琼张志春郭慧芳方继朝
Owner JIANGSU ACAD OF AGRI SCI
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