The invention discloses a
glutathione synthetase culture process. The
glutathione synthetase culture process comprises the following steps: A, a
fermentation section: preparing materials, culturing seeds, performing pretreating, performing fermenting and performing centrifuging; and B, a curing section: performing pulping, performing homogenizing and breaking walls, performing centrifuging, performing heat treatment, performing
nanofiltration, performing loading, performing mixing and
dewatering, and performing airing at low temperature, and airing mixed and dewatered loaded
polyester foam sheets in an airing chamber at low temperature of 20 DEG C to obtain the finished product of a
glutathione synthetase preparation. Through the mode, the
glutathione synthetase culture process particularly limits raw materials, performs high-temperature sterilization on a
test tube, a
shake flask, a
fermentation tank and a culture medium, and particularly limits the sterilization temperature and time of the
fermentation tank and the culture medium, so that the problem of infectious microbe infection is reduced, and increasing the yield is facilitated; and airing at low temperature is adopted, so that the production difficulty and cost are reduced.