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Preparation process of L-carnosine synthetase

A preparation process and technology for synthesizing enzymes, which is applied in the field of L-carnosine synthetase preparation process, can solve the problems of not being able to meet the requirements of industrialized production, few types of carnosine hydrolase, long reaction time, etc., and achieve low raw material prices and conversion process conditions Optimized, low production cost effect

Pending Publication Date: 2021-04-02
NANTONG ZILANG BIOPHARMA TECH CO LTD
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Problems solved by technology

[0007] However, in the prior art, the publicly reported carnosine hydrolase has few varieties, low catalytic activity, long reaction time, and low product concentration, which cannot meet the requirements of industrial production.

Method used

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  • Preparation process of L-carnosine synthetase

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Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] The invention provides a technical scheme: a preparation process of L-carnosine synthetase, using β-alanine and L-histidine hydrochloride as raw materials, MnCl 2 As a catalyst, L-carnosine hydrolase is added to catalyze the synthesis of L-carnosine by enzymatic reaction at pH 8.0-8.5 and temperature 35-40°C, including the following steps:

[0026] Step 1: using water as a reaction solvent, dissolving L-histidine hydrochloride in water to obtain a reaction solution;

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Abstract

The invention discloses a preparation process of L-carnosine synthetase. The preparation process comprises the following steps: by using beta-alanine and L-histidine hydrochloride as raw materials andMnCl2 as a catalyst, adding L-carnosine hydrolase, and performing enzymatic reaction catalysis at 35-40 DEG C under the pH value of 8.0-8.5 to synthesize L-carnosine. Aiming at the defects of the prior art in the enzymatic reverse hydrolysis synthesis of the L-carnosine catalyzed by the carnosine hydrolase, the conversion process conditions are optimized, the efficiency and the product concentration of the peptide hydrolase for synthesizing the L-carnosine are improved, and the preparation process has the advantages of low raw material price, short enzymatic conversion time, simplicity and convenience in operation, high product concentration, high conversion efficiency, low production cost, and the like.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation process of L-carnosine synthetase. Background technique [0002] L-carnosine (L-carnosine, N-β-Alanyl-L-histidine), also known as β-alanyl-L-histidine, molecular formula: C 9 h 14 N 4 o 3 , with a molecular weight of 226.23, is a dipeptide obtained by condensation of two amino acids, β-alanine and L-histidine, and is a crystalline solid. First discovered in beef in 1900, it is widely present in the brain, muscle and other tissues of mammals, and is a naturally active dipeptide. [0003] L-carnosine has anti-oxidation and anti-aging functions. It has therapeutic effects on hypertension, heart disease, senile cataracts, ulcers, etc., and has biological activities such as anti-tumor. Adding carnosine when fresh meat is stored can improve the flavor of the meat and prolong the shelf life. L-carnosine has been proven to scavenge reactive oxygen species (ROS)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/10
CPCC12P17/10
Inventor 张礼秦振华
Owner NANTONG ZILANG BIOPHARMA TECH CO LTD
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