A kind of one-step synthesis method of L-carnosine and truncated L-carnosine synthetase
A technology for synthesizing enzymes and carnosine, applied in biochemical equipment and methods, peptides, enzymes, etc., can solve the problems of low yield of L-carnosine, complex reaction products, difficult extraction and purification, etc. The effect of mass production
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[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0024] The preparation method of carnosine: using β-alanine, L-histidine, ATP and polyphosphate as raw materials, MgCl 2 As an activator, add L-carnosine synthetase and polyphosphate kinase to catalyze the synthesis of carnosine through enzymatic reaction coupling at pH 6.5-8.5, 30-45°C, such as figure 1 shown. Specific steps:
[0025] 1. Construction of recombinant truncated L-carnosine synthetase strain
[0026] 1.1: Extract the amino acid sequence from 480 to 930 of carnosine synthase1 encoded by the gene accession number GU453679 in Genbank, and add MERKT peptide to the amino acid to form a truncated L-carnosine synthase amino acid sequence, such as SEQ ID NO As shown in .3, after codon optimization, an NcoI restriction site is added to the 5' end of the optimized sequence, and an XhoI restriction site is added to the 3' end to...
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