30 results about "Hyaluronan synthase" patented technology

Disclosed are DNA segments encoding <DEL-S DATE="20010821" ID="DEL-S-00001">hyaluronic acid synthase which are employed to construct recombinant cells useful in the production of hyaluronate synthase or hyaluronic acid (HA)<DEL-E ID="DEL-S-00001"> <INS-S DATE="20010821" ID="INS-S-00001">the recombinant DNA segment identified in FIG. 5. <INS-E ID="INS-S-00001">In preferred aspects, chromosomal DNA from Streptococcus equisimilis is partially digested with EcoRI and the resultant fragments are ligated to form recombinant vectors. These vectors are useful in the transformation of host cells such as E. coli and or Streptococcal hosts. <DEL-S DATE="20010821" ID="DEL-S-00002">Resultant transformants are screened by the novel screening assays to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies may be selected and employed in the production of the enzyme itself or its product, HA.<DEL-E ID="DEL-S-00002"> <INS-S DATE="20010821" ID="INS-S-00002">The recombinant DNA segment identified in FIG. 5 is then inserted into a recombinant Streptococcal host for the production of hyaluronic acid (HA).<INS-E ID="INS-S-00002">

The present invention relates to a recombinant Bacillus host cell containing a recombinant vector including a nucleic acid segment having a coding region segment encoding enzymatically active hyaluronan synthase (HAS). The recombinant Bacillus host cell is utilized in a method for producing hyaluronic acid (HA).

The present invention relates to methods for producing a hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton, the methods comprising the steps of:

• (a) cultivating a recombinant Bacillus host cell at a first temperature conducive to its growth, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence;
• (b) then cultivating the recombinant Bacillus host cell of step (a) at a second temperature higher than the first temperature of step (a) under conditions suitable for production of the hyaluronic acid, whereby the Bacillus host cell produces hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton; and (b) recovering the hyaluronic acid.

The invention discloses a method for producing hyaluronic acid with different molecular weights through reasonable regulation, belonging to the technical field of bioengineering. The method comprises the following steps: integrating hyaluronic synthase hasA from streptococcus zooepidemicus on a bacillus subtilis genome; performing modularized assembly and expression analysis on genes of UDP-GlcA and UDP-GlcNAc synthetic routes in bacillus subtilis, and producing hyaluronic acids with different molecular weights from the bacillus subtilis by controlling different UDP-GlcA and UDO-GlcNAc concentrations. The method disclosed by the invention can be used for laying a certain foundation for efficiently preparing the hyaluronic acids within a specified molecular weight range and is suitable for industrial production and application.

The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida. The present invention also relates to coatings for biomaterials wherein the coatings provide protective properties to the biomaterial and/or act as a bioadhesive. Such coatings could be applied to electrical devices, sensors, catheters and any device which may be contemplated for use within a mammal. The present invention further relates to drug delivery matrices which are biocompatible and may comprise combinations of a biomaterial or a bioadhesive and a medicament or a medicament-containing liposome. The biomaterial and/or bioadhesive is a hyaluronic acid polymer produced by a hyaluronate synthase from Pasteurella multocida. The present invention also relates to the creation of chimeric molecules containing hyaluronic acid or hyaluronic acid-like chains attached to various compounds and especially carbohydrates or hydroxyl containing substances. The present invention also relates to a chondroitan synthase from Pasteurella multocida which is capable of producing polysaccharide polymers on an acceptor or primer molecule.

The invention discloses a recombinant Pichia pastoris for producing small-molecular hyaluronic acids and a construction method thereof, belonging to the technical field of bioengineering. Hyaluronic acid synthase hasA derived from Streptococcus zooepidemicus and UDP-glucose dehydrogenase tuaD derived from Bacillus subtilis are expressed in recombinant Pichia pastoris GS115 host to implement production of hyaluronic acids. Meanwhile, hyaluronidase derived from hirudo is integrated to the Pichia pastoris genome and subjected to secretion expression by constitutive promoters with different intensities; the secretion expression level of the hyaluronidase is controlled to prepare small-molecular hyaluronic acid products with different molecular weights; and the prepared products have different ranges of molecular weight, and have instruction and reference meanings for directly producing small-molecular hyaluronic acids within a specified range from microbes. The recombinant Pichia pastoris lays certain foundation for high-efficiency preparation of small-molecular hyaluronic acids, and is suitable for industrial production.

The present invention relates to a recombinant Bacillus host cell containing a recombinant vector including a nucleic acid segment having a coding region segment encoding enzymatically active hyaluronan synthase (HAS). The recombinant Bacillus host cell is utilized in a method for producing hyaluronic acid (HA).

The invention provides an engineering strain for producing high-molecular-weight hyaluronic acid, a construction method thereof and application of the engineering strain in producing the high-molecular-weight hyaluronic acid. The engineering strain is bacillus subtilis, and the Latin scientific name of the engineering strain is Bacillus subtilis; the engineering strain is named as BH-2, and the preservation number of the strain is CGMCC No.16841; the engineering strain selects bacillus subtilis WB600 as a basic strain, a hyaluronic synthase hasA gene, a UDP-glucose dehydrogenase has B gene anda UDP-glucose pyrophosphorylase hasC gene are introduced into the genome of the engineering strain, the characteristic of the low protease activity of the engineering strain is maintained, the growthand metabolism of the engineering strain are controlled through temperature, the high-molecular-weight hyaluronic acid with the molecular weight of 6.9*106 Dalton is obtained, and the engineering strain has industrial application value.

A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. Methods of producing hyaluronic acid utilizing a recombinant host cell having an expression construct encoding the functionally active hyaluronan synthase with altered enzymatic activity are also disclosed.

The invention relates to use of plant fermented extracts, especially relates to the use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronansynthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells. The invention provides the imperata cylindrica fermented extract by performing two-stage fermentation on an imperata cylindrica water extract with saccharomycetes and lactic acid bacillus. The invention further provides the uses of the imperata cylindrica fermented extract for preparing a composition enhancing the expression quantity of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells.The imperata cylindrica fermented extract can effectively improving the moisturizing capacity of skin, maintaining the arrangement of keratinocyte and the completeness of cuticle structure, thus improving the shield function of skin, making skin compact and elastic, and preventing aging of skin.

Provided for are three novel isoenzyme variants of the human Hyaluronan Synthase gene, HAS1Va, HAS1Vb, and HAS1Vc. Also provided is a method for assessing disease, or susceptibility to disease, in a patient; in which a cell or sample of a cell population is mixed with at least one compound which specifically binds to HAS1Va, HAS1Vb, HAS1Vc or HAS2 isoenzyme or isoenzyme variant genomic mRNA transcript or products arising therefrom; and methods of treatment.