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Use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells

A technology of Rhizoma Imperatae root and fermented products, applied in the field of plant fermented products, can solve the problems of sensitization, decreased water-holding capacity, fragile skin, etc., and achieve the effects of increasing moisturizing capacity, preventing skin aging, and promoting the proliferation of collagen and elastin.

Pending Publication Date: 2020-05-22
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the above, in response to the modern trend of emphasizing natural health, in order to improve the problem of skin becoming fragile and sensitive due to the damage of keratinocytes and the decrease of water retention capacity, we developed a product that can effectively make keratinocytes secrete more moisturizing factors. It is really necessary to maintain the arrangement of keratinocytes, maintain the integrity of the structure of the stratum corneum, and improve the skin barrier function.

Method used

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  • Use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells
  • Use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells
  • Use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 The preparation method of the fermented product of coriander root of the present invention

[0038] In one embodiment of the present invention, thoroughly clean the roots of Imperata corydalis, mix the washed roots with water at a solid-to-liquid weight ratio of 1:18-30, and sterilize and extract at 50-100°C for 0.5-3 hours. To obtain the water extract of Imperata rhizome, which is cooled to room temperature for the subsequent two-stage fermentation, firstly, 0.01-0.5% (v / v) of yeast (Saccharomyces cerevisiae, purchased from Biological Resource Conservation and Research Center, Taiwan, China, No. BCRC20271) was fermented at 20-37° C. for 1-3 days in the water extract of Imperata coronata, and then directly added 0.01-0.25% (v / v) Lactobacillus (Lactobacillus plantarum TCI028, purchased at Biological Resource Conservation and Research Center, Taiwan, China, No. BCRC910805) was fermented at 20-37°C for 1-3 days; wherein, the fermentation of these two kinds of ...

Embodiment 2

[0039] Example 2 The fermentation step of the present invention improves the effect of the total polysaccharide content in the water extract of Imperata coronata

[0040] In this example, in order to compare whether the total polysaccharide content in the fermented product of Imperata imperinae according to the present invention is higher than that in the water extract of Imperata imperinae, the phenol-sulfuric acid assay (Phenol-sulfuric acid assay) is used to quantify the concentration of total polysaccharides in the sample. When the polysaccharide encounters a strong acid, the hydroxyl group on the structural formula will combine with the phenol to produce an orange liquid, so the concentration of the total polysaccharide in it can be detected by colorimetry (especially the absorbance value of the sample at 490nm). First, accurately weigh 10 mg of D-glucose (D-Glucose, purchased from J.T. Baker, USA, No. 1916-01) into a 10 mL volumetric flask, and then add ddH 2 0 until the...

Embodiment 3

[0045] Example 3 Efficacy of the Fermented Product of Imperatae Rhizoma of the present invention in Improving the Expression of KRT14 Gene, FLG Gene, HAS2 Gene and HAS3 Gene

[0046] In the present invention, human primary epidermal keratinocytes (HPEK) are used to regulate the expression of TGM1 gene, KRT1 gene, KRT10 gene, KRT14 gene, AQP3 gene, FLG gene, HAS2 gene, and HAS3 gene expression of the fermented product of Rhizoma Imperatae according to the present invention. quantitative analysis. The human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) No. HPEK-50, and the cells were cultured in serum-free keratinocyte medium (keratinocyte-SFM) (Gibco, No. #17005042, U.S.), and contained 5%CO 2 cultured in a 37°C cell culture incubator.

[0047] will be 1.5×10 5 HPEK-50 cells were cultured in a six-well culture dish containing 2 mL of the above cell culture medium. After culturing at 37°C for 24 hours, the culture medium was removed without disturbing ...

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Abstract

The invention relates to use of plant fermented extracts, especially relates to the use of imperata cylindrica fermented extract for enhancing the gene expression of keratin, filaggrin and hyaluronansynthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells. The invention provides the imperata cylindrica fermented extract by performing two-stage fermentation on an imperata cylindrica water extract with saccharomycetes and lactic acid bacillus. The invention further provides the uses of the imperata cylindrica fermented extract for preparing a composition enhancing the expression quantity of keratin, filaggrin and hyaluronan synthase, promoting the proliferation of collagen and elastin, and enhancing antioxidant capacity of skin cells.The imperata cylindrica fermented extract can effectively improving the moisturizing capacity of skin, maintaining the arrangement of keratinocyte and the completeness of cuticle structure, thus improving the shield function of skin, making skin compact and elastic, and preventing aging of skin.

Description

technical field [0001] The present invention relates to the use of plant fermented products, especially the use of a fermented product of Imperata imperinae for improving gene expression, promoting collagen and elastin hyperplasia, and improving antioxidant capacity. Background technique [0002] The epidermis is the outermost layer of the skin. From the outside to the inside, it consists of the stratum corneum, granular layer, spinous layer, and basal layer. The epidermis is mainly formed by continuous upward differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization. The water content in corneocytes is high. As the cells metabolize and differentiate upward, the shape of corneocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells without nuclei and organelles will form in the stratum corneum. The main function of the epidermis is to keep the skin water...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P17/00A61P17/18A23L33/10
CPCA61K36/899A61P17/00A61P17/18A23L33/10A61K2236/19A23V2002/00A23V2400/11A23V2200/30A23V2200/318A23V2250/76
Inventor 林咏翔
Owner TCI CO LTD
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