30 results about "Histological examination" patented technology

The present invention is directed to a system for use in the preparation in situ of tissue specimens for histological examination. The system includes a cassette, a specimen mounting card and a base mold. The invention also is directed to a method of processing a tissue sample for histological examination which does not require re-orienting the selected cutting plane in the specimen transfer step. Instead, the tissue sample remains oriented in a desired position throughout processing.

The present inventors investigated the effects of anti-IL-6 receptor antibodies on improving the condition of infarcted areas in myocardial infarction, and on suppressing left ventricular remodeling after myocardial infarction. As a result, the administration of anti-IL-6 receptor antibodies significantly suppressed the increase of MPO activity in the infarcted area and suppressed myocardial MCP-1 expression in both the infarcted area and the non-infarcted area. Furthermore, echocardiography and histological examinations revealed that cardiac hypertrophy is also suppressed.

The present inventors investigated the effects of anti-IL-6 receptor antibodies on improving the condition of infarcted areas in myocardial infarction, and on suppressing left ventricular remodeling after myocardial infarction. As a result, the administration of anti-IL-6 receptor antibodies significantly suppressed the increase of MPO activity in the infarcted area and suppressed myocardial MCP-1 expression in both the infarcted area and the non-infarcted area. Furthermore, echocardiography and histological examinations revealed that cardiac hypertrophy is also suppressed.

The present invention relates to a gripper device for transporting a rack (3) for microscope slides (4), in particular in systems for preparing tissue samples for histological examinations, the gripper device having a gripping mechanism including a pair of gripping arms (2), whose ends are configured as gripping sections (7) for gripping the rack. In order to provide a gripper device which can be used in a versatile manner in automated stainers and coverslippers and contributes to reducing the cost of manufacture, it is proposed that the gripping sections (7) be provided with first profiles (17) for gripping the rack (3) in a vertical position and second profiles (18) for gripping the rack (3) in a horizontal position.

The present invention is directed to an assembleable unit of cassettes for containing specimens for histological examination. The cassettes are each designed for stacking relation one to another by snap locking means which are preferably configured to provide an audible sound and “click” feeling when stacked. A cover is provided to complete a stack, thereby providing an integral unit for case management. The unit is so integrated that it can be handled and will not disengage the stacked cassettes in the usual handling process in the laboratory. It is also readily processed in a paraffin bath for preparing the contained specimens for microtomy. Thus, a stabilized unit is provided for multiple specimens from a single source and maintaining them in sequential order without individual handling.

A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.

A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.

The invention provides a conjugate of a polymer and a biological stain and a preparation method and application of the conjugate. The conjugate has the formula G-X-B, wherein the polymer G is selected from the group consisting of glucans, fructosans, polymannuronic acid, polyguluronic acid, heteropolysaccharides, mono- or hetero-polysaccharides, mono- or hetero-polyuronic acid polysaccharides, mono- or hetero-polyuronic acid sulfate esters, hydrophilic alcohol high polymers, polylysine, polyglutamic acid and polyaspartic acid; the linking group X is a C1-C10 organic group; and the biological stain or a derivative B thereof may be toluidine blue, methylene blue, Evans blue, neutral red, Janus green or bright cresol purple. The conjugate can stain bilateral neck and submaxillary or submental lymphatic vessels and lymph nodes in New Zealand white rabbits by indirect lymph staining method, without diffusion in tongue body. No obvious toxic and adverse effects appear in all experimental animals in each group, and no obvious pathological changes appear in histological examination of internal organs.

An interactive apparatus, system and method for image capturing and projecting is integrated into an optical microscope. An image capturing and projecting unit is operatively connected to a processing unit, the processing unit configured to: (a) receive user generated data; and (b) overlay the user generated data onto an optically viewed image visible through the eyepiece.

The invention discloses a human Crohn disease-simulating murine colitis model, and a preparation method and use thereof. The preparation method comprises the following steps of: (1) establishing the murine colitis model by using trinitro-benzene-sulfonic acid (TNBS) enema; (2) evaluating the establishment of the model by observing general physical signs and observing the activity status of a mouse in real time; (3) after the mouse is killed, detecting clinical indexes, taking the whole colon of the mouse and performing length measurement, disease scoring and histological examination to evaluate the establishment of the murine colitis model; and (4) taking the splenocyte of the mouse and performing T-cell subset distribution checking to determine the change of immunocytes of the model. The invention provides a support for researches on the detection of clinical colitis mediation, and lays a foundation for development and research of effective treatment medicaments.

The invention discloses an application of perillaldehyde in preparation of a medicine for preventing and treating vaginitis, and belongs to the technical field of medicines. The invention further discloses a medicine for preventing and treating candida albicans vaginitis, which is a preparation prepared by using perillaldehyde as an active ingredient with pharmaceutically acceptable auxiliaries. The perillaldehyde has a relatively strong effect for inhibiting candida albicans. An in-vivo experiment is carried out by adopting a mouse immunosuppression model to evaluate the effect of perillaldehyde for preventing and treating candida albicans vaginitis; and fungus inspection and tissue histopathologic examination prove that perillaldehyde has relatively strong effects for preventing and treating candida albicans vaginitis, and can be used for preparing the medicine for preventing and treating candida albicans vaginitis.

A histological specimen retaining device for processing tissue. The histological specimen retaining device comprises a foldable permeable sheet having edges and a permeable target disposed on the foldable permeable sheet within the edges of said sheet thereby providing extended flap portions. The extended flap portions are foldable to overlap the target. The histological specimen retaining device also comprises a malleable securing strip attached to the permeable sheet of a length sufficient to secure said folded flap portions overlapping said target.

The invention provides a preparation method of a Rag1 gene defect animal model, which utilizes a gene editing technology to destroy a Rag1 gene coding region of a mouse, detects the model through immune system index evaluation and pathological histological examination, and verifies that the obtained mouse model is successfully constructed. The immunodeficiency model is helpful for research in thefields of tumor transplantation, immunology, inflammation and the like.

A method for active interference of danshinolic acid B to the animal model of oral cancer includes such steps as creating the animal model of oral cancer, interferring it by danshinolic acid B, histological examination, and pathologic evaluation. Its advantages are long interference time (12 weeks) and playing the full role of danshinolic acid B.

The invention discloses a preparation method of an animal model with chronic atrophic gastritis. The preparation method comprises: filling mice with N-methyl-N-nitro-nitrosoguanidine, wherein ammoniawater is used as drinking water, carrying out proliferation on helicobacter pylori, wherein the concentration is adjusted through a BHI medium, perfusing each mouse in the experimental group with helicobacter pylori, wherein before intragastric administration, the mice fast and after intragastric administration, the mice are subjected to fasting and water deprivation, selecting the male and femalemice, carrying out pathological histological examination on gastric mucosa, separating and culturing helicobacter pylori from the gastric mucosa, and confirming helicobacter pylori colonization and inflammation formation. The preparation method has the advantages of short cycle, high success rate, stable pathological change, strong reproducibility and outstanding substantive features, and can provide a good animal model for studying diseases such as chronic atrophic gastritis and gastric cancer caused by helicobacter pylori infection.

The invention discloses an anti-tumor experimental animal model and a construction method thereof. The construction method comprises the steps that a cancer specimen of a patient is immediately placed in an RPMI-1640 culture solution after being in vitro, tumor tissue is trimmed into tumor blocks, meanwhile, a prepared normal nude mouse is subjected to general anesthesia, diphenytoin sodium is diluted with normal saline, then intraperitoneal injection of the diphenytoin sodium is conducted, an incision is obliquely formed in the right rib, the abdominal cavity is opened, transplanted visceral organ cavities can be exposed, prepared human cancer tissue blocks are implanted, blood is sticked with cotton swabs, bleeding stopping is realized, sterilizing is carried out with alcohol cotton balls after obvious bleeding is avoided, incisions are continuously sutured, surrounding bloodstains can be wiped, the material is sterilized, and put back into a cage, and viscera is taken for histological examination when a preset observation period is reached. According to the anti-tumor experimental animal model and the construction method thereof, a cancer patient sample is directly taken out from a patient to construct the animal model, the occurrence condition of human tumors can be restored to the maximum extent, tumor heterogeneity is achieved, and the construction method is low in cost, short in period and good in repeatability.

The invention relates to building for a femoral epicondyle tuberculosis animal model, in particular to building of a rabbit femoral epicondyle tuberculosis animal model by using an H37Rv tuberculosisstrain. The model is an ideal platform for evaluating the effect of a drug delivery system in vivo. According to the method, the rabbit femoral epicondyle tuberculosis animal model is built by using the H37Rv tuberculosis strain, and the model is the ideal platform for evaluating the effect of the drug delivery system in vivo. The effectiveness and feasibility of the model are evaluated through gross specimen observation, Micro-CT examination, histological examination, mycobacterium culture and DNA micro-array testing. The femoral epicondyle animal model has the following advantages that the trauma is small in the preparation process, the operation is safe, and the model facilitates implantation of a material.

A method for the active interference of thalidomide to animal model of oral cancer includes such steps as creating the animal model of oral cancer, interferring it by thalidomide, histological examination and pathological evaluation. Its advantages are long interference time (12 weeks) and playing the full role of thalidomide.

The invention discloses a pemphigus vulgaris animal model construction method, which comprises the following steps: carrying out primary immunization / booster immunization on a wild type mouse by using a vaccination technology and taking desmoplasmin 3 as a vaccine antigen, starting to appear phenotypes within 28-35 days, including blisters, epidermis exfoliation and skin ulceration on the rear feet and the tail of the mouse with the overall phenotype; a serum autoantibody; the pemphigus vulgaris immunotherapy kit has the advantages that pemphigus vulgaris changes such as epidermis spinous layer debonding and antibody deposition in histological examination are simulated, part of mice have disease phenotypes such as local skin unhairing, fester and canthus conjunctivitis, clinical symptoms of pemphigus vulgaris are simulated, and an important tool is provided for pemphigus vulgaris immunotherapy.

The present invention relates to a method for preparing cell samples for histological examination and, more particularly, a method for preparing a cell sample as a standard, or control, for use in the fields of cytology and histology.

The invention discloses an injectable modified hyaluronic acid, a preparation method and a composition thereof. The modified hyaluronic acid is partially substituted by the -OH in the -COOH in the hyaluronic acid structural unit, and the substitution rate is not less than 40%. The modified HA of the invention can directly react with chitosan to form a hydrogel without chemical cross-linking reaction and will not destroy the HA structure. Rheological experiments show that AHA-CS can be made into injections, and the formed hydrogel structure is relatively stable. Animal experiments have shown that AHA-CS hydrogel can accelerate wound healing, promote cell proliferation and promote keratinocyte migration. In hydrogel-treated wounds, histological examination also demonstrated that the hydrogel promoted granulation tissue and capillary formation. In gel-treated wounds, the expressions of angiogenic factors (VEGF-A), chemokines (SDF-1), extracellular matrix remodeling factors MMPs and other MRNAs were increased or upregulated. More significantly, newly formed hair follicles were found on the gel-treated wounds.

Beta taipoxin obtained the taipoxin component of the venom of the taipan snake, Oxyuranus s. scutellatus, was fragmented by trypsin digestion. The fragment which showed the highest activity on skin cells for mitogenic activity and on PC12 cells for neurite growth was named Oxynor peptide. This fragment consisted of 21 amino acids and had the sequence from the N-terminal: Lys Gly Gly Ser Leu Leu Asn Phe Ala Asn Leu Ile Glu Asn Asp Val Pro Ile Asp His Met. Synthetic Oxynor peptide was constructed using ten amino acids (Ser Leu Leu Asn Phe Ala Asn Leu Ile Glu) and five amino acids (Ser Leu Leu Asn Phe). Experimentally produced 4 mm punched wounds on the back of the mice were treated with the ten amino acid version of synthetic Oxynor peptide. The results showed complete closure of the wounds after six days, while the wounds of controls remained open. The histological examination of the skin around the wounds showed epidermis almost like normal skin.

The invention provides a conjugate of a polymer and a biological stain and a preparation method and application of the conjugate. The conjugate has the formula G-X-B, wherein the polymer G is selected from the group consisting of glucans, fructosans, polymannuronic acid, polyguluronic acid, heteropolysaccharides, mono- or hetero-polysaccharides, mono- or hetero-polyuronic acid polysaccharides, mono- or hetero-polyuronic acid sulfate esters, hydrophilic alcohol high polymers, polylysine, polyglutamic acid and polyaspartic acid; the linking group X is a C1-C10 organic group; and the biological stain or a derivative B thereof may be toluidine blue, methylene blue, Evans blue, neutral red, Janus green or bright cresol purple. The conjugate can stain bilateral neck and submaxillary or submental lymphatic vessels and lymph nodes in New Zealand white rabbits by indirect lymph staining method, without diffusion in tongue body. No obvious toxic and adverse effects appear in all experimental animals in each group, and no obvious pathological changes appear in histological examination of internal organs.