30 results about "Halorubrum halophilum" patented technology

The invention discloses a microbial agent containing halophilic denitrifying bacteria YL5-2 and application of the microbial agent. The microbial agent contains the halophilic denitrifying bacteria YL5-2, and the YL5-2 is a novel species of Halovibrio genus, and is preserved in the China General Microbiological Culture Collection Centre with a preservation number of CGMCC NO.16315 on August 20, 2018. The invention further discloses application of the microbial agent in high-salt wastewater treatment. The YL5-2 can be used for biological denitrification treatment of high-salt wastewater with salt content greater than 10%, and denitrification treatment cost of the wastewater is reduced. According to the invention, the microbial agent prepared from the halophilic denitrifying bacteria YL5-2 can be used for degradation, conversion and biological denitrification processes of pollutants under a high-salt condition, and the processes comprise high-salt wastewater treatment, polluted seawatergovernance, saline-alkali land repair, nitrogen nutrition consumption, inhibition on excessive multiplication of alga, water body purification, substrate improvement, and the like.

The invention aims at providing a halomonas sulfidaeris. The strain is named Halomonas sulfidaeris YZ7, and the strain is preserved in China Center for Type Culture Collection of Wuhan University, Wuhan, China in 6th, March, 2013, and the preservation number of the strain is CCTCC NO:M2013070. Halomonas sulfidaeris YZ7 disclosed by the invention has the capability of inhibiting algae blooms, and the strain is simple in nutrient requirement, so that the strain can be developed for an aquaculture microbial preparation and a waste water treatment agent, and the application prospect is wide.

Halophilic marine gram negative bacteria, isolated from decayed algae, hydrolyzed κ-carrageenan. To maximize κ-carrageenase production in the cost effective manner, a novel medium was defined having minimum components and their optimum concentration by statistical optimization method. Hence there evolved a novel medium composition for enhanced κ-carrageenase production by a new halotolerant marine bacterium, Pseudomonas spp.

The invention belongs to the field of bioengineering, and particularly relates to a preparation method of recombinant halophilic archaea protease. The method comprises the following steps: constructing a recombinant plasmid by taking a genome of Halostella sp. DL-M4 as a template and taking a pET28a plasmid as an expression vector; transforming escherichia coli cell by recombinant plasmid, inducing expression, centrifugally collecting thalli for cell resuspension and crushing, centrifuging again, taking a supernatant, adding beta-mercaptoethanol to obtain a mixed solution, enabling the mixed solution to pass through a nickel column, combining zymogen with a nickel medium for renaturation, and finally performing secondary impurity protein removal and target protein elution, performing ultrafiltration centrifugal concentration and performing gel filtration chromatography to obtain the recombinant halophilic archaea protease. The method provided by the invention has good universality, simpler purification steps and shorter production cycle, the obtained protease has high purity, and finally the halophilic archaea protease with specific enzyme activity of 1159.15 U / mg is obtained.

The invention discloses a preparation method of fermented bean curd. The preparation method comprises the following steps of: S1, preparing soybean milk: soaking soybeans, grinding the soybeans into thick liquid, filtering the thick liquid, boiling the thick liquid, and sieving the thick liquid to obtain bean dregs and soybean milk; S2, preparing a blank: curdling the soybean milk with beneficial bacterium acid water with the acidity of 0.7-1.0 g / 100ml, squeezing the curdled soybean milk, and performing medium emulsification to obtain a white blank; S3, spraying seeds: uniformly mixing 5-15% of lactococcus lactis or tetragonococcus halophilus with 85-95% of mucor strains to obtain a beneficial bacterium mixed bacterium solution, inoculating the beneficial bacterium mixed bacterium solution to the white blank, and performing fermentation to obtain a blank after the fermentation is mature; S4, performing pickling: pickling the blank to obtain a salted blank; and S5, performing after fermentation: uniformly mixing 5-10% of tetragonococcus halophilus or lactococcus lactis with 90-95% of semi-finished product juice to obtain beneficial bacterium juice, filling the salted blank with the beneficial bacterium juice to form a semi-finished product, and performing after fermentation. According to the preparation method disclosed by the invention, the quality problems of white points, gas production, juice leakage and the like are controlled in a targeted manner, and fermented bean curd products with stable quality are produced.

The invention discloses bacillus halophilus for improving the salt resistance of nile tilapia and application of the bacillus halophilus. An ordinary seawater liquid medium of the bacillus halophilusis activated under the aseptic condition; activated bacterial liquid is subjected to enlarging cultivation, and a cultured culture solution is an inoculating solution; and after twice cleaning with sterilized phosphoric acid buffer solution, resuspension is conducted to prepare a cell suspension, the cell suspension is sprayed evenly on basal feed of the nile tilapia, and thus the concentration ofthe feed is increased to 108 cfu / g. According to the bacillus halophilus and the application thereof, the bacillus halophilus 221 is added to the basal feed of the nile tilapia, the digestibility andabsorption ability of the nile tilapia living in the environment with salinity being 1.6% is significantly improved, the antioxidant ability and the antibacterial ability can be improved, and a favorable foundation is laid for the provided quality of the tilapia; and meanwhile, great significance to improve the compound feed of the tilapia is also achieved.

The invention discloses a biological flocculant, which belongs to the field of environment-friendly microorganisms and is prepared from the following raw materials in parts by weight: 70 to 90 parts of bacillus halophilus LH-B.0015 fermentation liquor, 5 to 15 parts of bentonite, 1 to 5 parts of diatomite, 1 to 5 parts of ferrous sulfate, 1 to 5 parts of magnesium carbonate and 2 to 5 parts of calcium chloride. The invention also discloses a preparation method and application of the biological flocculant. The salt tolerance range of the halophilic bacillus amyloliquefaciens LH-B.0015 contained in the biological flocculating agent is 1%-30%, and the flocculating agent can be synthesized within the salinity range of 1%-25%. The strain is used for biological flocculation in a high-salt environment, can effectively solve the flocculation problem in the high-salt environment, reduces the treatment cost, and has a good application prospect.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a strain of novel Tetragenococcus halophilus and application of the novel Tetragenococcus halophilus. The Tetragenococcus halophilus is screened from traditional naturally-fermented soybean paste in Northeast, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 20, 2021, wherein the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 23165. The strain can greatly improve the yield of umami peptide, is beneficial to reducing the production cost of the umami peptide, and promotes the wide application of the umami peptide in the food field.

The invention relates to the field of sewage treatment, and discloses a halophilic brevibacterium linum strain capable of efficiently degrading ammonia nitrogen and application of same, wherein the brevibacterium linum is named as B1, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on July 2, 2019, the preservation number of the brevibacterium linum is CGMCC NO.18298, and the microorganism classification of the brevibacterium linum is named as Brevibacterium lines. The brevibacterium linum B1 has strong ammonia nitrogen degradation capability, can tolerate a high-salt environment, can grow and maintain high ammonia nitrogen removal efficiency under a salinity condition of 1%-10%, can be applied to high-salt sewage ammonia nitrogen degradation treatment, and has the advantage of short treatment time.

The invention relates to a method for cultivating halophilic granular sludge, which belongs to the field of sewage biological treatment. The principle of the invention is as follows: (1) rapidly increasing the influent load to stimulate EPS secretion of halophilic microorganisms. Microorganisms attach and grow through the binding of EPS, and form mature halophilic active granular sludge under the action of hydraulic shear force; (2) The combined effect of long and short settling time. The long precipitation time in the early stage can achieve the growth of halophilic microorganisms in the reactor; the short precipitation time in the later stage can provide selective pressure for granulation. The halophilic activated granular sludge cultivated in the present invention has various sludge sources and simple technology. Since the enriched microorganisms come from the halophilic bacteria group deposited in the estuary, the salinity tolerance range is between 1% and 10%. And can retain the compact shape structure. It greatly shortens the precipitation time, effectively shortens the treatment cycle and ensures the treatment effect, realizes the rapid treatment of high-salt wastewater, realizes short-range nitrification, and reduces energy consumption by 30%.

The invention provides a method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria, which comprises the following steps: culturing a marine bacteria XS1412C strain in advance, inoculating the XS1412C strain into a fermentation culture solution to prepare an XS1412C strain fermentation culture solution, and culturing halophilic lactic acid bacteria in advance to produce alginate lyase. The method comprises the following steps: inoculating halophilic lactic acid bacteria into an XS1412C strain fermentation culture solution for symbiotic fermentation culture to obtain a symbiotic fermentation culture solution, and finally centrifuging the symbiotic fermentation culture solution to obtain an enzyme solution containing alginate lyase. Halophilic lactic acid bacteria capable of generating beneficial substances through fermentation, promoting growth of strains and improving enzyme production capacity of the strains and XS1412C strains capable of secreting alginate lyase are subjected to symbiotic fermentation culture, secretion of alginate lyase can be promoted, enzyme quality can be guaranteed, enzyme activity can be effectively improved, the fermentation process can be simplified, fermentation time can be shortened, and extraction cost can be reduced. The invention belongs to the technical field of marine organisms.

The invention provides a halophilic autotrophic desulfurized bacterial strain having the maximum tolerance to a 14% mixed solution of Na2CO3 and NaHCO3. The bacterial strain is named as NPE-1, and the preservation number is CCTCC NO: M 2015152. The bacterial strain can normally grow in the 7%-10% mixed solution of Na2CO3 and NaHCO3, hydrogen sulfide is converted into elemental sulfur, and the desulphurization rate can be up to 0.2-0.3 g(H2S) / (L.h). The bacterial strain has very good hydrogen sulfide load-resisting capacity and high pH resistance performance, can normally grow under the pH condition of 10.5-11 and can treat gas with the hydrogen sulfide concentration of 5000-20000 ppm. A generation cycle is short and is only 40 minutes, and the sundry bacterial resistance is strong. The invention further provides application of the halophilic desulfurized bacterial strain in biological desulphurization. The halophilic desulfurized bacterial strain can efficiently convert the hydrogen sulfide into the elemental sulfur, and also has very high halophilism, accordingly wastewater discharge and alkali liquid consumption are remarkably reduced, the operation cost of biological desulphurization is greatly reduced, the stability of a biological desulphurization system is improved, a solid foundation is laid for industrial application of a washing tower type biological desulphurization technology, and the bacterial strain is suitable for large-scale popularization and application.

The invention discloses halophilic salinicola sp.W-Y12 and application thereof. The classification name of the halophilic salinicola sp.W-Y12 is Salinicola sp. The strain was preserved in China General Microbiological Culture Collection Center on November 13th, 2018 with a preservation number of CGMCC No.16742. An experiment proves that the halophilic salinicola sp.W-Y12 disclosed by the inventioncan emulsify crude oil and can be used for microbe oil recovery to improve the oil recovery rate; in addition, the halophilic salinicola sp.W-Y12 also can be used for treating and repairing of petroleum pollution and has a good application prospect.

The invention aims at providing a halomonas sulfidaeris. The strain is named Halomonas sulfidaeris YZ7, and the strain is preserved in China Center for Type Culture Collection of Wuhan University, Wuhan, China in 6th, March, 2013, and the preservation number of the strain is CCTCC NO:M2013070. Halomonas sulfidaeris YZ7 disclosed by the invention has the capability of inhibiting algae blooms, and the strain is simple in nutrient requirement, so that the strain can be developed for an aquaculture microbial preparation and a waste water treatment agent, and the application prospect is wide.

The invention discloses a microbial deodorant, which is prepared from halophilic fish bacillus LH-B.0015, and further prepared from halophilic denitrifying bacteria YL5-2 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.16315. The microbial deodorant has the advantages that the microbial deodorant is prepared from the halophilic fish bacillus LH-B.0015; the invention further discloses application of the microbial deodorant. The microbial deodorant disclosed by the invention is remarkable in deodorization effect, can tolerate or degrade organic pollutants, and is particularly suitable for treating amine-containing odor and odor generated in a salt-containing environment. The microbial deodorant has the advantages of simple treatment process, stable effect, environmental poison impact resistance and salinity influence resistance, and has a very wide application prospect in foul gas pollution control.

The invention discloses a method for evolution and domestication of halophilic microalgae, and belongs to the field of biomass resource development and utilization. The method for evolution and domestication of the halophilic microalgae comprises the steps that the halophilic microalgae is separated into salt algae colonies, and then activation is carried out; a multi-step adaptive evolution method is used for reducing cell loss of the halophilic microalgae caused by sudden change in osmotic pressure, and repeated domestication is carried out on the microalgae by a LED photobioreactor. According to the method for evolution and domestication of the halophilic microalgae, the salinity setting is adjusted and controlled, the environmental pressure is changed reasonably, and the adaptive evolution of the halophilic microalgae is achieved, so that the lutein production under seawater-like salinity culture conditions is improved. The method for evolution and domestication of the halophilic microalgae has the characteristics of environmental friendliness and is beneficial to the utilization of marine resources and environmental protection, adaptive evolution and domestication of the halophilic microalgae driven by environment stress is carried out by gradually reducing the salinity, so that the level of lutein produced by salt algae is significantly improved, and the good industrial prospect is achieved.

The invention discloses a halophilic halobacterium W-Y12 and its application. The classification of the halophilic halobacterium W-Y12 of the present invention is named as the salinefield fungus Salinicola sp., and the strain has been preserved in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures on November 13, 2018, and the preservation number is CGMCC No. .16742. It is proved by experiments that the halophilic halobacterium W-Y12 of the present invention can emulsify crude oil, and can not only be used for microbial oil recovery, improve oil recovery rate, but also be used for the control and repair of oil pollution, and has a good application prospect.

The invention belongs to the technical field of bioengineering, and in particular relates to a halophilic archaea strain for degrading nitrite and its application. The present invention found a Haloarcula A new species of the genus, named Haloarcula sp. JB33. The strain is a facultative aerobic denitrifying bacteria, which can grow at a salt concentration of 5-25% and a temperature of 20-52°C, and has good salt tolerance. The optimum growth temperature is 37°C. When the salt concentration is 18%, and the nitrite concentration is 100mg~500mg / kg, it still grows normally and degrades nitrite effectively, and the optimal degradation rate reaches 98.59% after 96 hours of degradation. Its degradation effect of nitrite is obvious. It can be used to degrade nitrite in food. It is especially used in the degradation of nitrite in high-salt pickled foods such as kimchi and ham.

The invention discloses a microbial agent containing halophilic denitrifying bacteria YL5‑2 and an application thereof. A microbial agent containing halophilic denitrifying bacteria YL5‑2, YL5‑2 is a new species of the genus Halovibrio, preserved in the China General Microorganism Culture Collection and Management Center, the preservation number is CGMCC NO.16315, and the preservation date is 2018 August 20. The application of the microbial agent of the invention in the treatment of high-salt wastewater. YL5‑2 can be used for biological denitrification treatment of high-salt wastewater with a salt content greater than 10%, reducing the cost of denitrification treatment of such wastewater. The microbial bacterial agent prepared by the halophilic denitrifying bacteria YL5-2 disclosed by the present invention can be used in the degradation, transformation and biological denitrification process of pollutants under high-salt conditions, including high-salt wastewater treatment, polluted seawater treatment, saline-alkali land restoration, Consume nitrogen nutrients, inhibit algae overgrowth, purify water, improve substrate, etc.

The invention relates to the field of sewage treatment, and discloses a halophilic Brevibacterium flaxenum capable of efficiently degrading ammonia nitrogen and an application thereof. The Brevibacterium flaxenum is named B1, and it has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on July 2, 2019. Its preservation number is CGMCC NO.18298, and the microbial classification is named Brevibacterium flaxenum Brevibacterium lines . The Brevibacterium flaxenum B1 has a strong ability to degrade ammonia nitrogen and can tolerate high-salt environments. It can grow and maintain a high removal efficiency of ammonia nitrogen under the condition of 1%-10% salinity, and can be applied to ammonia nitrogen in high-salt sewage Degradation treatment, and has the advantage of short treatment time.

The invention provides a high-salt low-concentration organic wastewater treatment method which comprises the following steps: adding activated and domesticated halophilic strain liquid into a biological contact oxidation device, and controlling the reaction time of the biological contact oxidation device and the adding amount of the activated and domesticated halophilic strain liquid, organic substances in the high-salt low-concentration organic wastewater are removed. The technical problem of how to treat the high-salt low-concentration organic wastewater at low cost is solved, and the treatment method of the high-salt low-concentration organic wastewater, provided by the invention, can provide a biological treatment way for treating the high-salt low-concentration organic wastewater with the salt content of 15-30%; the biological treatment efficiency of organic substances in high-salinity water is greatly improved; the investment cost of treatment facilities of the high-salt low-concentration organic industrial wastewater is greatly reduced; the operation cost in the high-salt low-concentration organic wastewater treatment process is greatly reduced; the tolerance concentration of microbial strains to salt substances is greatly improved, and the tolerance concentration of organisms to salt is improved by more than 15 times.

The invention relates to the field of gene engineering, in particular to xylanase Scxyn5 and a coding gene and application thereof. The amino acid sequence of the gene is as shown in SEQ ID NO: 1. The invention provides xylanase Scxyn5 of Schizophyllum commune, the xylanase Scxyn5 is an extremely salt-tolerant / halophilic xylanase, and a high-salt-concentration catalytic system can improve the hydrolytic activity of the xylanase to corn viscose, so that the corn viscose can be degraded by the xylanase Scxyn5, and the corn viscose can be degraded by the xylanase Scxyn5, so that the corn viscose can be degraded by the xylanase Scxyn5. Arabinoxylan with different component force distribution characteristics can be prepared by applying xylanase single enzyme to hydrolyze corn viscose, and a basis is provided for industrially preparing functional arabinoxylan with specific molecular weight by taking corn bran as a raw material.

The invention belongs to the field of gene engineering, and relates to a preparation method and application of a halophilic archaea extracellular protease truncated body. The method comprises the following steps: transforming a prokaryotic host by using a recombinant plasmid pET28a-hlyA, carrying out induced expression to obtain halophilic archaea protease, carrying out purification and renaturation on the halophilic archaea protease, and incubating to obtain the high-purity halophilic archaea extracellular protease truncation HlyA CTE of which the amino acid sequence is that a mature enzyme sequence of HlyA lacks a CTE structural domain (SEQ ID No.1). The HlyA CTE has excellent enzymatic characteristics, and particularly has good stability in high-temperature, low-salt, high-salt, alkaline and other environments; meanwhile, the HlyA CTE can be used for catalyzing hydrolysis of protein substrates, including catalysis of macromolecular protein substrates represented by azocasein, is specifically applied to fish sauce fermentation to promote hydrolysis of fish protein, and provides a novel enzyme preparation for protein degradation in high-salt, high-temperature and other environments.

The invention belongs to the field of bioengineering, in particular to a method for preparing a recombinant halophilic archaeal protease; the steps are as follows: using the genome of Halostella sp.DL-M4 as a template and pET28a plasmid as an expression vector to construct a recombinant plasmid; transforming the recombinant plasmid into Escherichia coli The cells were induced to express, and the cells were collected by centrifugation for cell resuspension and fragmentation. The supernatant was obtained by centrifugation again, and β-mercaptoethanol was added to obtain a mixed solution, which was passed through a nickel column, and the zymogen was combined with the nickel medium to renature. Protein and target protein elution, ultrafiltration centrifugal concentration, gel filtration chromatography to obtain recombinant halophilic archaeal protease; the invention has good versatility, simpler purification steps, shorter production cycle, and high purity of the obtained protease. The halophilic archaeal protease with a specific enzyme activity of 1159.15 U / mg was finally obtained.