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A kind of composite bacterial strain for treating high-salt wastewater

A technology of compound bacteria and Clostridia, applied in biological water/sewage treatment, microbial-based methods, water/sludge/sewage treatment, etc., can solve microbial growth and toxicity, threats and impacts of human life and production, and standard sewage Surrounding environmental pollution and other problems, to achieve the effect of stable performance of single bacteria

Active Publication Date: 2020-07-28
宜兴国际环保城科技发展有限公司
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Problems solved by technology

[0002] In recent years, with the vigorous development of my country's industrial industry, the discharge of industrial sewage has increased rapidly. With the diversification of chemical synthesis processes, most of the high-salt and high-chlorine sewage has been added to the industrial sewage. The growth of microorganisms can be inhibited or poisonous, and the discharge of a large amount of standard sewage will inevitably pollute the surrounding environment and receiving water bodies, endangering the surrounding sea areas, thus posing a serious threat and impact on human life and production

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  • A kind of composite bacterial strain for treating high-salt wastewater

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Embodiment 1

[0025] Embodiment 1: the preparation of composite strain

[0026] Described compound bacterial species comprises bacillus, nitrifying bacteria, streptococcus, legionella, clostridium, cyanobacterium, pseudomonas, denitrifying bacteria, sulfur bacteria and actinomycetes, preferably, described compound bacterial species comprises by percentage Bacillus 83.3%, Nitrifying bacteria 5%, Streptococcus 1%, Legionella 1%, Clostridium 1%, Cyanobacteria 1%, Pseudomonas 2.5%, Denitrifying bacteria 3.5%, Sulfur bacteria 1.2% and Actinomycetes 0.5%, more preferably, the bacillus is a genetically modified bacillus, and the composite bacterial agent can be prepared into liquid bacterial agents, dry powder bacterial agents, capsules, etc. Bacteria count not less than 2.8×10 7 cfu / mL; when prepared as a dry powder inoculum, the count of viable bacteria in the dry powder inoculum shall not be less than 1.2×10 8 cfu / g; when prepared into capsules, the count of viable bacteria in the capsules sh...

Embodiment 2

[0027] Embodiment 2: the acquisition of total halophilic gene

[0028] Saline-alkaline lakes are natural high-saline environments, most of which are inland lakes, which can form a relatively closed microbial ecological environment. The microbial communities of saline-alkali lakes include algae, cyanobacteria, aerobic bacteria, anaerobic bacteria, facultative aerobic bacteria, Archaea, etc. Archaea are an important component of the microbial community of saline-alkaline lakes. They adapt to high-saline environments, and most of them belong to the chemoorganotrophic type. Because halophilic archaea have different osmotic pressure, cell membrane ion distribution, and The degree of halophilicity and oligotrophy are difficult to cultivate under conventional conditions. It is precisely because of the specificity of the above-mentioned physical and chemical properties that archaea have metabolic pathways that are different from conventional microorganisms, and have the potential to be...

Embodiment 3

[0031] Example 3: Construction of a halophilic gene library

[0032] The purified DNA was sequenced and the Escherichia coli expression system (E.coli DH5a) was used to construct the salt lake halophilic gene library, and the genomic DNA was randomly interrupted to 100bp, 150bp, 200bp, 250bp, 500bp, 1.0kb, 1.5kb, 2.5kb After connecting the DNA fragments with adapters, PCR amplified 100bp, 150bp, 200bp, 250bp, 500bp sequences and sequenced them on the machine to obtain short sequence libraries. The 1.0kb and 1.5kb large fragment libraries were constructed using Cre-Lox library construction technology. The two ends of the large DNA fragment are connected with LoxP adapters and then circularized and sequenced; according to the genome construction steps, the insert fragments of 250bp, 500bp, 1.0kb, and 1.5kb are respectively constructed into the library, and the genome sequencing entrusts Nanjing GenScript to perform SOLEXA sequencing The platform is complete. The above-mentioned...

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Abstract

The invention provides a composite strain for treating high-salt wastewater. In particular, the method comprises transferring a halophilic related gene into a specific microorganism; after the obtained halobacteria are added into a deep biochemical pool, the microbial life in background sludge in the deep biochemical pool is not affected. The halobacteria are resistant and degradable to high-salt,high-chlorine, high-alkali sewage, and have a function of treating sewage having poor biodegradability, the single bacteria performance is stable, and the performance can be passed through genetic modification, which is specially suitable for industrial scenes with deep sewage treatment needs.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a composite strain for treating high-salt wastewater. Background technique [0002] In recent years, with the vigorous development of my country's industrial industry, the discharge of industrial sewage has increased rapidly. With the diversification of chemical synthesis processes, most of the high-salt and high-chlorine sewage has been added to the industrial sewage. The growth of microorganisms can be inhibited or poisonous, and the discharge of a large amount of standard sewage will inevitably pollute the surrounding environment and receiving water bodies, endanger the surrounding sea areas, and cause serious threats and impacts on human life and production. [0003] Bioaugmentation technology belongs to biological treatment technology. Bioaugmentation technology refers to adding microorganisms with specific functions to the biological treatment system to improve the treatment ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/01C12R1/46C12R1/145C12R1/38C12R1/04
Inventor 周春松周佳玮贾建洪王浩孙坚
Owner 宜兴国际环保城科技发展有限公司
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