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Biological flocculant as well as preparation method and application thereof

A technology of biological flocculants and uses, applied in the aforementioned biological flocculants and their preparation methods and uses, in the field of biological flocculants, to achieve the effect of good application effect and high flocculation activity

Active Publication Date: 2022-03-29
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there are few reports on the flocculation ability of halophilic bacteria, especially under the condition of salinity ≥ 15%, the bacteria that can achieve flocculation

Method used

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  • Biological flocculant as well as preparation method and application thereof
  • Biological flocculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1, Isolation and Preservation of Bacillus halophilus LH-B.0015

[0047] Bacillus halophilus LH-B.0015 was isolated from sea bottom mud.

[0048]Prepare LB liquid medium with a NaCl concentration of 20%, add 250 mg / L glycerol, 250 mg / L grape, and 50 mg / L methanol, and enrich the samples for 48 hours at 30°C. The strains in the enriched culture medium were isolated by using YL solid medium. 1L medium contains the following components: glucose: 0.6g, trisodium citrate 0.5g, glycerol 2mL, phenol 0.1g, aniline 0.05g, peptone 1.6g, dipotassium hydrogen phosphate 0.35g, potassium dihydrogen phosphate 0.1g, Ammonium sulfate 0.25g, ammonium chloride 0.25g, MgSO40.5g, CaCl2 0.1g, NaCl 180g; trace element solution 10mL, pH7.0-7.2; agar 2.5%.

[0049] Trace element solution (1000mL): MnCl 2 ·7H 2 O 0.03g, H 3 BO 3 0.03g, CoCl 2 ·6H 2 O 0.20g, CuCl 2 ·6H 2 O, NiCl 2 ·6H 2 O 0.02g, Na 2 Mo 4 2H 2 O 0.03g

[0050] The strain LH-B.0015 was deposited in the Gene...

Embodiment 2

[0051] Example 2, preparation of fermentation product of Bacillus halophilus LH-B.0015

[0052] (1) Culture medium: glucose 250mg / L, sucrose 500mg / L, glyceraldehyde 200mg / L, sodium chloride 100-250g / L, cellulose 100mg / L, starch 250mg / L, sodium acetate 250mg / L, ammonium sulfate 500mg / L, a small amount of trace elements, pH 7.0-9.0. The liquid medium is sterilized while controlling water evaporation.

[0053] (2) Put the above medium into a 1L Erlenmeyer flask, inoculate Bacillus halophilus LH-B.0015 and ferment at a temperature of 35°C and a salt concentration of 1%, 10%, 15%, 20%, and 25%. Cultivate, supplement the loss of water during the cultivation process, and prevent the change of salt concentration. After 48 hours of culture, the OD600 value was measured under the conditions of 1%, 10%, 15%, 20%, and 25% salt concentration, and the culture was transferred twice, and each transfer was cultured for 48 hours.

[0054] (3) Inoculate the culture solution in the 1L Erlenmey...

Embodiment 3

[0056] Embodiment 3, the preparation of biological flocculant

[0057] Get 70 parts of fermentation supernatants of 1%, 10%, 15%, 20%, 25% salinity Bacillus halophilus LH-B.0015 obtained in Example 2, add 15 parts of bentonite, diatomaceous earth 5 parts, ferrous sulfate 3 parts, magnesium carbonate 2 parts, calcium chloride 5 parts. After stirring and mixing, after spraying and drying at a temperature of 45-55° C., bioflocculant 1, bioflocculant 2, bioflocculant 3, bioflocculant 4, and bioflocculant 5 are obtained.

[0058] Get 80 parts of the fermentation supernatant of the 1%, 10%, 15%, 20%, 25% salinity Bacillus halophilus LH-B.0015 that embodiment 2 makes, add 10 parts of bentonite, diatomaceous earth 3 parts, 3 parts ferrous sulfate, 2 parts magnesium carbonate, 2 parts calcium chloride. After stirring and mixing, after spraying and drying at a temperature of 45-55° C., bioflocculant 6, bioflocculant 7, bioflocculant 8, bioflocculant 9, and bioflocculant 10 are obtaine...

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Abstract

The invention discloses a biological flocculant, which belongs to the field of environment-friendly microorganisms and is prepared from the following raw materials in parts by weight: 70 to 90 parts of bacillus halophilus LH-B.0015 fermentation liquor, 5 to 15 parts of bentonite, 1 to 5 parts of diatomite, 1 to 5 parts of ferrous sulfate, 1 to 5 parts of magnesium carbonate and 2 to 5 parts of calcium chloride. The invention also discloses a preparation method and application of the biological flocculant. The salt tolerance range of the halophilic bacillus amyloliquefaciens LH-B.0015 contained in the biological flocculating agent is 1%-30%, and the flocculating agent can be synthesized within the salinity range of 1%-25%. The strain is used for biological flocculation in a high-salt environment, can effectively solve the flocculation problem in the high-salt environment, reduces the treatment cost, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of environment-friendly microorganisms, in particular to a biological flocculant. The present invention also further relates to the aforementioned biological flocculant and its preparation method and use. Background technique [0002] At present, microbial flocculants have been widely used in the fields of wastewater purification, fermentation and food processing. Compared with chemical flocculants, microbial flocculants are safe, non-toxic, efficient and environmentally friendly, making them have broad application prospects and attract more and more researchers' attention. Microbial flocculant is a biodegradable, safe, efficient, non-toxic and economical biopolymer obtained through microbial fermentation, cultivation and extraction. Flocculation can effectively remove 80%-95% of suspended matter and 65%-95% of colloidal matter, which can effectively reduce the COD value in water; and through flocculation purificati...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12P1/04C12R1/07
CPCC02F3/34C12P1/04Y02W10/10
Inventor 田凤蓉王开春孙文妮王强徐军张璐璐洪磊
Owner BLUESTAR LEHIGH ENG INST CO LTD
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