The invention discloses a method for biocatalytically synthesizing D-(+)-glucuronic acid and its application. In the method, sucrose is used as a substrate, and an enzyme is added to carry out a catalytic reaction to obtain D-(+)-glucuronic acid; wherein, the enzymes include sucrose phosphorylase, phosphoglucomutase, inositol-1-phosphate Synthetase, myo-inositol monophosphatase and myo-inositol oxidase, the enzyme catalyzed system can get a high conversion rate, in addition, by adding phosphate, phosphofructokinase and phosphoglucose isomerase to the above reaction system, it can be Further improve the conversion rate of D-(+)-glucuronic acid. The method of the invention has high raw material utilization rate, high conversion rate of D-(+)-glucuronic acid, simple steps, low production cost, little pollution and little impact on the environment, and can realize large-scale D-(+)-glucuronic acid Production.