Fructokinase-disabled cyanobacteria and application thereof in secretion and production of fructose
A fructokinase, cyanobacteria technology, applied in bacteria, applications, enzymes, etc., can solve the problems of not finding cyanobacteria to secrete fructose, failing to achieve industrial production, and not finding cyanobacteria.
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[0022] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.
[0023] 1. Algal strains used
[0024] The cyanobacteria Synechococcus PCC7942 and the cyanobacteria Synechococcus PCC7002 are often used as model strains for related experimental research. Wherein, the amino acid sequence of the fructokinase gene of PCC7942 is shown in SEQ ID NO.1, and the nucleic acid sequence is shown in SEQ ID NO.2; the amino acid sequence of the fructokinase gene of PCC7002 is shown in SEQ ID NO.3, and the nucleic acid sequence is shown in SEQ ID NO. 4.
[0025] 2. Plasmid construction
[0026] 2.1 Construction of the PCC7942 fructokinase knockout plasmid pJS11: design primers according to the PCC7942 fructokinase gene and its upstream and downstream sequences in the genome, and use the PCC794...
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