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Compound native microorganism domestication enzyme for efficiently improving urban black and odorous water body, and method for treating urban black and odorous water body

A technology for microbial domestication and black and odorous water bodies, which can be applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc. To achieve better regulation effect, high positive regulation efficiency, and regulation improvement effect

Active Publication Date: 2020-04-28
北京京阳环保工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above-mentioned treatments for black and odorous water bodies are all treated with biological methods, the microbial preparations sprayed are divided into broad-spectrum microorganisms and obligate microorganisms. Broad-spectrum microorganisms include aerobic microorganisms, facultative microorganisms, and anaerobic microorganisms. There are many types of microorganisms, but the content is not large. After being put into the water body, it cannot play a role because it does not adapt to the water body environment or cannot become the dominant bacterial group. It needs a large number of multiple dosages.

Method used

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  • Compound native microorganism domestication enzyme for efficiently improving urban black and odorous water body, and method for treating urban black and odorous water body
  • Compound native microorganism domestication enzyme for efficiently improving urban black and odorous water body, and method for treating urban black and odorous water body

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Effect test

Embodiment 1

[0050] A compound native microbial domestication enzyme that efficiently improves urban highly polluted black and odorous water bodies. The compound native microbial domestication enzyme is domesticated through the following steps:

[0051] S1-1: Sampling the strains of Clostridium, Bacillus and Methanogens in the culture solution, the amount of glucose added to the culture solution is 5g / L, anaerobic oxygen cultivation is carried out for 24h, and the culture temperature is 25°C , the content of composite microorganisms in the obtained primary seed liquid is 0.8g / L;

[0052] S1-2: Then inoculate the above-mentioned culture solution into a new culture solution with an inoculum amount of 6% by volume and carry out secondary anaerobic culture for 72 hours at a culture temperature of 25°C;

[0053] S1-3: Subsequently, the above-mentioned culture medium was subjected to cell disruption, followed by membrane filtration, and the supernatant was obtained after filtering out cell debri...

Embodiment 2

[0065] A compound native microbial domestication enzyme that efficiently improves urban highly polluted black and odorous water bodies. The compound native microbial domestication enzyme is domesticated through the following steps:

[0066] S1-1: Sampling the strains of Clostridium, Bacillus and Methanogenus in the culture solution for anaerobic training for 72 hours, the culture temperature is 30°C, and the content of composite microorganisms in the obtained primary seed solution is 1g / L;

[0067] S1-2: Then inoculate 10% inoculum volume of the above-mentioned culture solution into a new culture solution for secondary anaerobic culture for 120 hours at a culture temperature of 30°C;

[0068] S1-3: Subsequently, the above culture medium was subjected to cell disruption, followed by membrane filtration, the filter membrane had a pore size of 0.45um, and the supernatant was obtained after filtering out cell debris;

[0069] S1-4: Vacuum freeze-dry the obtained supernatant to o...

Embodiment 3

[0079] A compound native microbial domestication enzyme that efficiently improves urban highly polluted black and odorous water bodies. The compound native microbial domestication enzyme is domesticated through the following steps:

[0080] S1-1: Sampling the strains of Clostridium, Bacillus and Methanogenus in the culture solution for anaerobic training for 48 hours, the culture temperature is 28°C, and the content of composite microorganisms in the obtained primary seed solution is 0.9 g / L;

[0081] S1-2: Then inoculate the above-mentioned culture solution into a new culture solution with an inoculum amount of 8% by volume to carry out secondary anaerobic culture for 96 hours, and the culture temperature is 28°C;

[0082] S1-3: Subsequently, the above culture medium was subjected to cell disruption, followed by membrane filtration, the filter membrane had a pore size of 0.45um, and the supernatant was obtained after filtering out cell debris;

[0083] S1-4: Vacuum freeze-dr...

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Abstract

The invention discloses a compound native microorganism domestication enzyme for efficiently improving an urban black and odorous water body, and a method for treating the urban and odorous water body. The compound native microorganism domestication enzyme comprises acetyl CoA acyltransferase, 1-phosphofructokinase, pyruvate phosphate dikinase, beta-hydroxybutyryl coenzyme dehydrogenase, 3-phosphoglycerate kinase, NAD-dependent beta-hydroxybutyl coenzyme A dehydrogenase, and thiolase. The method for treating the urban black and odorous water body comprises the following steps: preparation andactivation of the compound native microorganism domestication enzyme; putting of the activated compound native microorganism domestication enzyme into the black and odorous water body for a period oftime; and then aquatic plant construction. The compound native microorganism domestication enzyme of the invention has the advantages of efficiently improving the quality of the black and odorous sewage water body. In addition, the treatment method of the invention has the advantages of efficiently purifying the water body, and continuously adjusting and optimizing the water body environment.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, more specifically, it relates to a compound local microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies. Background technique [0002] Urban rivers are not only channels for urban flood control and drainage, but also an important factor in urban landscape and urban ecological environment. They have multiple functions such as flood control and drainage, water supply and drainage, urban landscape, and ecological balance. With the rapid development of urbanization in my country, rivers have been polluted by pollution sources for a long time, resulting in a decline in water quality, and with the intensification of pollution, the self-purification function of water bodies is lost, and urban rivers are black and smelly, becoming a veritable " Smelly Ditch". [0003] The governance and restora...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N9/04C12N9/12C12N9/00C02F3/34C12R1/145C12R1/07C12R1/125C12R1/01
CPCC12N9/1029C12N9/1205C12N9/0006C12N9/1217C12N9/1294C12N9/00C02F3/342C12Y203/01009C12Y207/01056C12Y101/01157C12Y207/02003C12Y207/09001
Inventor 霍石磊林绚丽王丽妹钟本帅
Owner 北京京阳环保工程有限公司
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