A composite native microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies
A technology for microbial domestication and black and odorous water, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low content, inadaptability to the water body environment, and inability to become a dominant bacterial group. Achieve the effects of promoting growth metabolism, high positive regulation efficiency, and enhancing biodiversity
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Embodiment 1
[0050] A composite native microbial domestication enzyme for efficiently improving urban high-pollution black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:
[0051] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled into the culture solution, the amount of glucose added to the culture solution was 5g / L, anaerobic culture was carried out for 24h, and the culture temperature was 25°C , the content of composite microorganisms in the obtained primary seed liquid is 0.8g / L;
[0052] S1-2: Then inoculate the above-mentioned culture solution into a new culture solution according to the inoculum volume of 6% of the volume for a second anaerobic culture for 72 hours, and the culture temperature is 25°C;
[0053] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, and the cell debris is filtered to obtain a supernatan...
Embodiment 2
[0065] A composite native microbial domestication enzyme for efficiently improving urban highly polluted black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:
[0066] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled in the culture solution for anaerobic cultivation for 72 hours, the culture temperature was 30°C, and the content of compound microorganisms in the obtained primary seed solution was 1 g / L;
[0067] S1-2: then inoculate the above-mentioned culture solution into a new culture solution according to the volume of 10% of the inoculum for secondary anaerobic culture for 120 hours, and the culture temperature is 30°C;
[0068] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, the pore size of the filter membrane is 0.45um, and the supernatant is obtained after filtering off cell debris;
[0069] S...
Embodiment 3
[0079] A composite native microbial domestication enzyme for efficiently improving urban highly polluted black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:
[0080] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled in the culture solution for anaerobic cultivation for 48h, the culture temperature was 28°C, and the content of compound microorganisms in the obtained primary seed solution was 0.9 g / L;
[0081] S1-2: then inoculate the above-mentioned culture solution into a new culture solution according to the volume of 8% of the inoculum for secondary anaerobic culture for 96 hours, and the culture temperature is 28°C;
[0082] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, the pore size of the filter membrane is 0.45um, and the supernatant is obtained after filtering off cell debris;
[0083] S1-4: t...
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