The invention discloses a high-protein oil-immersed tuna can and a processing technology thereof. The high-protein oil-immersed tuna can is prepared from tuna meat, peanut kernels, walnut kernels, pearice, sunflower seeds, refined vegetable oil, black sesame seeds and refined salt. The peanut kernels, the walnut kernels, the pea rice and the sunflower seeds are added into the tuna meat, so that the protein content of the canned oil-immersed tuna is increased, the taste and flavor of the canned oil-immersed tuna are improved, the nutritional ingredients are more comprehensive, and the eating requirements of different consumers are met; fishy smell removing liquid is prepared from water, cooking wine, ginger juice, fineleaf schizonepeta herb juice and ground pepper, and is used for soakingtuna meat, so that the meat quality of tuna is improved, the taste of tuna meat is improved, the fishy smell of tuna which spoils appetite is removed, and the quality of the canned oil-immersed tuna is improved, black sesame is cooperated with refined salt and refined vegetable oil for stir frying, therefore, the fragrance of the canned oil-immersed tuna is improved, the sense and taste buds of consumers are stimulated, and the appetite of the consumers is improved.