41 results about "Endocellulases" patented technology

A Clostridium thermocellum thermostable cellulase enzyme with both endocellulase activity and exocellulase activity that is able to degrade cellulose in the absence of scaffolding and other cellulosomic proteins is provided. The use of the enzyme to degrade cellulosic materials to soluble sugars is also provided.

Disclosed herein are cellulase compositions useful as papermaking performance additives for improving paper dry strength of a paper product and reducing refining energy in papermaking processes, and improving paper production. These cellulase compositions are formulated using cellulase, papermaking contaminant control polymers, protein stabilizers and cellulase enhancers. These cellulase compositions measure higher in endo-cellulase activity with better stability than conventional cellulase, and have shown differentiating performance in improving paper dry strength properties versus cellulase alone.

The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.

The present invention discloses one kind of clostridium and its culture process and application. The clostridium is rumen clostridium H1 in the preservation number of CGMCCNo.2125, and has high culture efficiency and contained cellulose degrading composite enzyme system comprising exocellulase, endo cellulase, xylanase, mannanase, esterase and pectase. The rumen clostridium H1 has capacity of decomposing natural cellulose, esterase activity of decomposing ester, easy medium temperature culture at 39 deg.c and capacity of utilizing pentose. It may find its wide application in cellulose degrading industry.

A Clostridium thermocellum thermostable cellulase enzyme with both endocellulase activity and exocellulase activity that is able to degrade cellulose in the absence of scaffolding and other cellulosomic proteins is provided. The use of the enzyme to degrade cellulosic materials to soluble sugars is also provided.

The present invention discloses one kind of clostridium and its culture process and application. The clostridium is rumen clostridium H1 in the preservation number of CGMCCNo.2125, and has high culture efficiency and contained cellulose degrading composite enzyme system comprising exocellulase, endo cellulase, xylanase, mannanase, esterase and pectase. The rumen clostridium H1 has capacity of decomposing natural cellulose, esterase activity of decomposing ester, easy medium temperature culture at 39 deg.c and capacity of utilizing pentose. It may find its wide application in cellulose degrading industry.

The invention concerns an enzymatic method for making oenological tannins starting with lumps of wood and an enzymatic method for transforming tannins into tannins for wine-making purposes. The inventive method comprises a step which consists in contacting the lumps of wood or tannins with an aqueous solution comprising a composition containing enzymes of the cellulase class. The invention also concerns an enzymatic composition mainly consisting of enzyme of the cellulase class for making oenological tannins, comprising an endocellulase activity, a xylanase activity a beta-mannanase and / or alpha-amylase activity.

The invention belongs to the technical field of gene engineering, in particular to a new salt resistant endo cellulase and a coding gene thereof. The invention discloses a sequence and a coding area of the gene; the sequence of the gene is shown as SEQ ID NO: 1; the full length of the sequence is 1053bp; and 350 amino acids are coded. Escherichia coli DH5 Alpha / S664 / 6p / cel8h containing a gene plasmid is preserved in China Center for Type Culture Collection (CCTCC); and the serial number of preservation is CCTCC NO:M208233. Biology validates and shows that the endo cellulase has strong tolerance to high saline salinity. The endo cellulase can be widely applied in high saline catalysis, cellulose fermentation and other industrial fields.

A preparation method for neutral esterase, and a stickies control enzyme reagent used for a waste paper papermaking process are disclosed. The stickies control enzyme reagent used for the waste paperpapermaking process includes, by weight, the following components: 50%-60% of the neutral esterase (enzyme activity>=10000 u / ml), 10%-15% of endoglueanase (enzyme activity>=5000 u / ml), 15%-25% of lipase (enzyme activity>=200 u / ml), and 20% of a stabilizer; ad wherein, the neutral esterase is prepared from fermentation of bacillus cereus. A stickies control enzyme provided by the invention can effectively hydrolyze vinyl acetate (PVAc) to make the vinyl acetate become a water-soluble low molecular substance. A hydrolyzed product can be stably dispersed on a fiber surface, and the surface viscosity is lowered, so that the possibility of agglomerating into large stickies particles is greatly reduced, and the particles can be attached to the fiber surface and taken out of a system with paper without causing quality losses of the paper, thereby enabling a phenomenon that stickies affects production to be effectively controlled. The invention also includes the preparation method of the neutral esterase.

The invention relates to a persistent endo-cellulase mutant with remarkably improved enzyme activity and application thereof. The mutant comprises 70th and / or 235th amino acid mutation of an amino acid sequence, and the amino acid sequence of the endo-cellulase is as shown in SEQ ID NO: 1. A single-point mutation technology is adopted, based on homologous modeling and molecular docking methods, key amino acids in an enzyme activity framework are selected to optimize the molecular structure of the persistent endo-cellulase, and the enzyme activity is improved. Compared with a wild type enzyme, the content of reducing sugar generated by degrading filter paper by the continuous endo-cellulase mutant is greatly increased, and the continuous endo-cellulase mutant has important significance on efficient degradation of a cellulose substrate and reduction of the production cost.

The invention provides aspergillus oryzae fermentation liquor and a preparation method of the aspergillus oryzae fermentation liquor. The aspergillus oryzae fermentation liquor contains manganese peroxide with the enzymatic activity being 100-10000U / L, endo-cellulase with the enzymatic activity being 150-3000U / L, beta-dextranase with enzymatic activity being 150-3000U / L, and beta-mannase with enzymatic activity being 50-2000U / L. The invention further provides a process technology of corn stalk sugar liquor. The corn stalk sugar liquor uses corn stalks as main raw materials and is prepared by adding a hydrogen dioxide solution to degrade the corn stalks through the aspergillus oryzae fermentation liquor. The process technology has the advantages of being short in production cycle, small in occupied space, low in production cost and high in sugar yield. In addition, the prepared corn stalk sugar liquor can be used as a carbon source raw material for producing butanol, ethyl alcohol and other fermentation industries.

The invention belongs to the technical field of gene engineering, in particular to a new salt resistant endo cellulase and a coding gene thereof. The invention discloses a sequence and a coding area of the gene; the sequence of the gene is shown as SEQ ID NO: 1; the full length of the sequence is 1053bp; and 350 amino acids are coded. Escherichia coli DH5 Alpha / S664 / 6p / cel8h containing a gene plasmid is preserved in China Center for Type Culture Collection (CCTCC); and the serial number of preservation is CCTCC NO:M208233. Biology validates and shows that the endo cellulase has strong tolerance to high saline salinity. The endo cellulase can be widely applied in high saline catalysis, cellulose fermentation and other industrial fields.

The invention relates to an endocellulase catalytic domain comprising the sequence of SEQ ID NO: 1 or a functionally equivalent variant of said catalytic domain that substantially maintains or improves its catalytic activity. The invention also relates to a polypeptide, a nucleic acid, an expression cassette, a vector or a host cell. Additionally, the invention relates to the use of an endocellulase catalytic domain or the polypeptide of the invention for hydrolysing cellulose, producing bioethanol or as a detergent. The invention also relates to a method for hydrolysing cellulose and for producing bioethanol.

An object of the present invention is to provide a soft biomass decomposition method, a production method for a target substance from soft biomass, and an enzyme or group of enzymes for decomposing soft biomass. Provided is a soft biomass decomposition method, including a step of bringing an enzyme selected from specific exocellulase, endocellulase, and processive endocellulase into contact with soft biomass such as bagasse and rice straw. Also provided is a production method for a target substance from soft biomass by incorporating the soft biomass decomposition method as a step. Further provided is an enzyme or group of enzymes for decomposing soft biomass selected from specific exocellulase, endocellulase, and processive endocellulase.

This invention is about preparation and application of beta-1,4- gluglucosan-6,2,3-sulfuric ester. This preparation is as followings: add cellulose into ion liquid, stir to form the homogeneous system of cellulose and ion, then add sulfation agent, stir at 30-35deg C and 800-1000r / min for 6-8hous. Add the alcohol-alkali liquor to precipitate the product, filter, wash to get raw esterification product. Dissolve it in the NaH2 (PO4)3-Na2H (PO4)3 buffer, PH 7.5. Add EGI to degenerate. We finally get beta-1,4- gluglucosan-6,2,3-sulfuric ester after hyperfiltration grading, condensation with depression and vacuum drying. It could be used as cosmetic additives.