The invention belongs to the field of drug analysis and particularly relates to a method of establishing a fingerprint spectrum of an infant diarrhea stopping drug preparation. According to the method, by taking the infant diarrhea stopping drug preparation as a detection object, the method of establishing the fingerprint spectrum of the infant diarrhea stopping drug preparation is established, common characteristic peaks including peak 1, peak 2 liquiritin, peak 3 ammonium glycyrrhizinate, peak 4, peak 5, peak 6, peak 7 and peak 8 are affirmed, the peak 2 liquiritin is selected as an internal reference peak in the fingerprint spectrum, and a relative retention time of each common characteristic peak is determined; and in addition, with combination of information of a plurality of chromatographic peaks in the fingerprint spectrum, the quality of the infant diarrhea stopping drug preparation can be comprehensively and rapidly detected, so that comprehensive quality detection and whole quality control of the infant diarrhea stopping drug preparation are facilitated, and improvement on the use safety and stability of the drug is facilitated. Simultaneously, the method of establishing the fingerprint spectrum of the infant diarrhea stopping drug preparation has the advantages of high precision, good repeatability, high stability and the like.