The invention relates to the technical field of pipe sinking engineering construction, and discloses a pipe sinking construction method of an inverted siphon culvert across a river section. The pipe sinking construction method comprises the steps of excavation of an underwater groove, construction of a gravel cushion, steel pipe preparation, steel pipe sinking, steel pipe hydraulic pressure test and backfilling of medium-coarse sand and dimension stones. Steel pipes are welded integrally on the land, the characteristics of high connecting opening quality, accurate alignment, high constructionspeed and guaranteed welding joint quality inspection are achieved, then overall sinking is performed in water, the process is relatively simple, the construction efficiency is high, the quality is guaranteed, the construction period is greatly shortened, the construction cost is effectively reduced, and the downstream urgent water demand is met; and meanwhile, no restriction of special weather iscaused, the risk of flood control is low, river rising does not need to be worried about, navigation is not affected, and the safety of the construction is greatly increased.