The invention relates to a sediment oxygen demand (SOD) determination device based on electromagnetic dynamic, and a using method. The SOD determination device comprises a testing room, a dynamic room, a magnetic rotor, a magnetic pole, a motor, a dissolved oxygen probe socket, support fixing interfaces and a storage battery, wherein the testing room is in a cylinder structure with the bottom end opened and the runway-shaped cross section; the dissolved oxygen probe socket is formed on one side of the top of the testing room; the support fixing interfaces are arranged at the two ends of the top of the testing room respectively; the dynamic room is a cylindrical barrel and reversely fixed at the top of the testing room; the motor connected with the magnetic pole is arranged in the dynamic room, and is externally connected with the storage battery by a connecting line; the magnetic rotor is placed in the testing room, and is in magnetic connection with the magnetic pole; a hand-held support is connected and fixed to allow the device to be submerged in water and to be filled with overlying water; the dissolved oxygen probe is inserted and sealed; the device is then fixed at the bottom of the water; overlying water circulation of the testing room is adjusted and controlled; and an SOD is determined. The device is simple in structure, easy to dismount, small and exquisite in size, and convenient to carry, and can effectively determine the SOD under the action of different hydrodynamic forces.