The invention discloses a process for extracting non-denatured II-type collagen from sturgeon cartilage. The process comprises the following steps: cleaning cartilage in sturgeon head and / or fishbonein a NaOH solution to remove adhesive substances on the surface of the cartilage, performing flushing to neutrality, then sending the cartilage after flushing into continuous heating equipment for cooking, performing freezing, then sending the cartilage after freezing into continuous drying equipment for drying, and performing grinding into a powder to obtain a compound containing the non-denatured II-type collagen. According to the preparation process, the non-denatured II-type collagen can be obtained, chondroitin sulfate and hyaluronic acid in the cartilage can be reserved, and the extraction technological process is simple. Compared with the prior art, the process shortens production period, has low input, has high yield, and can achieve large-scale production.