35 results about "Bone Marrow Smear" patented technology

The invention discloses a process method for bone marrow smear digitization. The process method comprises the following steps: acquiring related information of bone marrow smears, generating global images, and performing digital labeling after obtaining the to-be-digitized area as well as karyocyte collecting quantity and megakaryocyte classifying quantity; feeding the bone marrow smears into scanning equipment for performing shooting scanning: a to-be-digitized area generated by low-power lens scanning, and labeling and recognizing object observers therein; generating a spliced image in the scanning area; switching to megakaryocyte in the megakaryocyte classifying quantity generated by high-power lens scanning, and labeling and recognizing the megakaryocyte; generating the scanned megakaryocyte image; switching to karyocyte in the karyocyte collecting quantity in the to-be-digitized area generated by oil lens scanning, labeling and recognizing the karyocyte; generating the scanned karyocyte image; and generating digitalized smears of physical bone marrow smears. The artificial microscopy process is simplified; the consistency and standardization of the bone marrow examination report results are improved; and remote consultation is conveniently carried out, and the turn-round time of the physical smears is reduced.

The invention discloses a bone marrow cell proliferation degree automatic grading method and system, and belongs to the technical field of computational microimaging. The method comprises the following steps: performing image acquisition on a bone marrow smear to obtain an intensity graph and a phase graph; inputting the intensity graph and the phase graph into a convolutional neural network model, and respectively counting nucleated cells and nucleless cells in the bone marrow smear through the convolutional neural network model; and calculating the ratio of nucleated cells to nucleless cellsaccording to the counting result, and obtaining the grade of the bone marrow cell proliferation degree, so as to overcome the complex problem in manual counting and provide accurate cell counting forgrading of the bone marrow cell proliferation degree.

The invention provides a staining kit of micro megakaryocytes of a bone marrow smear, and a using method of the staining kit. The staining kit comprises a reagent tube filled with a Polymer Helper reagent, a reagent tube filled with a PBS (Phosphate Buffer Solution), a reagent tube filled with an antigen repairing solution, a reagent tube filled with a hydrogen dioxide solution, a reagent tube filled with diaminobenzidine, a reagent tube filled with hematoxylin, a reagent tube filled with acid alcohol of 1%, a reagent tube filled with ammonium hydroxide of 1%, a reagent tube filled with xylene, a reagent tube filled with alcohol, a reagent tube filled with CD41 monoclonal antibodies, a reagent tube filled with CD61 monoclonal antibodies, a reagent tube filled with universal CD41 and CD61 immunohistochemical antibodies, and a packing box for packing the reagent tubes in a separated and concentrated manner. The staining kit disclosed by the invention overcomes the defect that form cell smears are judged according to subjective experiences, and the interference of false positive of single CD antibody staining to results is avoided, so that the detection accuracy is improved.

The invention provides a bone marrow cell classification and identification method, device and system based on deep learning, and belongs to the field of medical image processing. The method comprises the following steps: (1) acquiring a low-power lens full-slice image of a bone marrow smear by using a low-power lens; (2) cutting the low-power lens full slice image of the bone marrow smear into a plurality of low-power lens small images with the same size as the high-power lens imaging view; (3) classifying the low-power mirror small images obtained in the step (2) by using a trained image classification model to obtain low-power mirror small images with good visual fields; (4) scanning and imaging the low-power lens small image with a good visual field by using a high-power lens to obtain a corresponding high-power lens image; (5) classifying and counting bone marrow cells in the high-power lens image obtained in the step (4) by using the trained target detection model; (6) predicting blood diseases according to classification and counting results of the bone marrow cells. Therefore, identification and classification of bone marrow cells can be reliably and automatically realized, and the accuracy of blood disease prediction can be improved.

The invention provides an automatic focusing scanning method and system based on the bone marrow cell glass slide. Automatic focusing scanning of a plurality of bone marrow smears is achieved, a digital image is formed, and remote reading of the same bone marrow smear can be achieved through a network. According to the automatic focusing scanning method for the bone marrow cell glass slide, the image definition is calculated in a specific image definition calculation mode and serves as an image feedback signal to adjust the position of a focus of bone marrow cell focusing imaging. The focusingprocess of bone marrow cell imaging is rapidly achieved, and the problem that the digitization speed and imaging definition of an existing scanning focusing system cannot be considered at the same time can be solved.

The invention discloses parasite staining fluid. The parasite staining fluid is prepared from, by weight, 5%-8% of eosin, 2%-5% of celeste, 3%-6% of methylene blue, 10%-50% of methyl alcohol, 0.01%-0.02% of neutral glycerine, 5%-6% of sodium chloride, 1%-2% of twain-20, 0.5%-1% of tocopherol, 0.2%-0.8% of nanometer pearl powder, 0.6%-0.9% of ethyl acetate and the balance water. The parasite staining fluid is suitable for staining of parasites, various microorganisms, blood cell smears, bone marrow smears, exfoliative cell smears, chromosome banding, cell apoptosis detection and the like, onlyone staining fluid is used, fixation is not required, fixation and staining are integrated, and multiple inspection items of the parasites, germs, nuclear heterogeneous cells, cell classifying counting and the like can be simultaneously conducted on a slice; a method is simple and quick, staining power is high, coloring is clear, the form is complete, distinguishing is easy, and the detection rateis high.

The invention discloses a storage box for bone marrow smears. The storage box comprises a box cover, a box body, a heating device and a rotating shaft, wherein the box cover is rotationally connected with the box body through the rotating shaft; the heating device is arranged at the bottom of the box body; a smear insert for clamping the smear is arranged on at least one side wall at the inner side of the box body; and an elastic press sheet is arranged in the smear insert. Compared with the prior art, the storage box for the bone marrow smears has the advantages that the bone marrow smears can be orderly placed; the bone marrow smears can also be horizontally placed; and the problem that cells fall off during placements of the bone marrow smears is avoided. Meanwhile, due to the adoption of the heating device arranged at the bottom of the box body, the bone marrow smears can be quickly dried in a state that the storage box is not needed to be opened and further the convenience and simpleness in operation are realized.

The invention relates to an automatic leveling method for bone marrow smear scanning, which comprises the following steps: (1) a mechanical arm grabs a bone marrow smear from a slide box, the bone marrow smear is moved below a laser range finder, and a square scanning area is selected on the bone marrow smear as a frame selection area; (2) the laser range finder firstly performs scanning range finding on the boundary of the left side of the frame selection area of the bone marrow smear and then performs scanning range finding on the boundary of the right side of the frame selection area of the bone marrow smear to form a data set; (3) the mechanical arm adjusts the bone marrow smear according to the difference value, measured in the step (2), of the left boundary and the right boundary of the frame selection area, the left boundary and the right boundary of the frame selection area are adjusted to the same height, and the distance value under the condition is measured. The bone marrow smear is leveled and then focused, accurate focusing of the bone marrow smear is achieved, the method is easy to operate, the distance serves as the unique standard of focusing, focusing in the bone marrow smear shooting process can be achieved, and the method is not affected by uneven thickness of the bone marrow smear and different cell sizes.

The invention proposes a minimally invasive bone marrow biopsy sampling needle. The sampling needle comprises a needle tube, first and second needle cores, wherein the tail of the needle tube is provided with a main handle, the tails of the first and second needle cores are provided with auxiliary handles, the front end of the first needle core is provided with a triangular pyramid head, the center of the first needle core is provided with a first channel opened to the tail end surface of the first needle core, three pyramidal faces of the triangular pyramid head are provided with connecting channels communicating with the first channel, the center of the second needle core is provided with a second channel opened to the opposite side, the inner cylindrical wall at a terminal end positionof the front end of the second needle core is provided with a separation cone, a cone tip of the separation cone faces the center of the second needle core, and the separation cone is a triangular pyramid. The sampling needle has the advantages that bone marrow smear examination and bone marrow biopsy can be simultaneously carried out in one puncture, the use is reliable, pains of a patient can beeffectively reduced, and a success rate of bone marrow extraction is high.

The invention belongs to the field of tumor molecular diagnosis, and particularly relates to application of fluorescence in-situ hybridization on bone marrow smears in multiple myeloma. The application specifically comprises the following steps: making a bone marrow fluid into bone marrow smears for Wright's staining, and selecting an area with more myeloma cells and good dispersion as an FISH (fluorescence in-situ hybridization) area; covering the FISH area with a fresh stationary liquid to fade the FISH area and fix the cells, washing the bone marrow smears in a 2*SSC solution of 56 DEG C for 10 min, rapidly rinsing the bone marrow smears in deionized water once, and then putting the bone marrow smears into gradient ethanol for dehydration and air-drying; adding a FISH probe into the FISH area for hybridization, dyeing by using a DAPI counterstaining agent, and carrying out microscopic examination on a fluorescence hybridization signal. The bone marrow smear FISH detection method isespecially suitable for the condition that the proportion of monoclonal abnormal plasma cells in flow immunotyping is lower than 20%, and the proportion of myeloma cells in bone marrow smear Wright'sstaining morphology is higher than 20%.

The utility model discloses a bone marrow smear storage device, and effectively solves the problem that an existing common slide storage box is inconvenient to find and take during observation by medical staff. The device comprises a box body, positioning plates are arranged in the box body, guide grooves are formed in the positioning plates, the guide grooves are composed of transverse grooves in the front-back direction and arc-shaped grooves, two moving rods are arranged between the two positioning plates, the right ends of the two moving rods are connected through guide columns, the guide columns are inserted into the guide grooves, inserting columns are arranged on the moving rods, the left ends of the two moving rods are connected through a box cover, an intermittent gear is rotationally connected in the box body, a transmission wheel is rotationally connected in the box body, a plurality of bases are arranged in front of the box body, a plurality of storage grooves are formed in the lower side wall of each base, driven wheels are arranged on the front side and the rear side of the right end of each base respectively, the driven wheel on the rearmost side is hinged to the transmission wheel through a driving rod, a plurality of connecting rods are arranged in front of the bases, and the driving rod and a rocker are respectively hinged with the connecting rods through bearing rods. The device is ingenious in structure and convenient to use.

The invention belongs to the medical field, providing an acetal phosphatide dyeing reagent group for a bone marrow smear and an application method thereof, wherein the reagent group using HgCl2 as the fixture and the Schiff reagent as the dyeing solution includes following reagents: the HgCl2 solution, the Schiff reagent, the sulfite water solution and the Mayer brazilein solution; the steps of the application method are as follows: (1) the bone marrow smear is fixed by the HgCl2; (2)the bone marrow smear is washed by distilled water; (3) the bone marrow smear is dyed by the Schiff reagent and washed by the sulfite water solution; (4) the bone marrow smear is dyed again by the Mayer brazilein solution and washed by flowing water; (5) the judgment of the dyeing result is as follows: cytolymph which is red is positive, and cytolymph which is not dyed is negative. The dyeing method of the invention has the advantages of easy realization, convenient observation of the dyeing result and extremely high specificity, and is an effective method for judging megakaryocytes in the acute leukemia M7 and other leukemia cells.

The invention mainly solves the technical problem of providing a bone marrow cell morphology analyzer system, and the system comprises a global box used for scanning and sampling the whole bone marrow smear and generating a QR code containing a bone marrow smear image and corresponding patient information; a QR code printer which is used for printing the two-dimensional code; a slide box which is used for storing bone marrow smears on which QR codes are pasted after information is collected by the global box; a bone marrow cell morphology analyzer which is used for performing focusing and scanning sampling on the bone marrow smear under different multiples; an acquisition end which is used for identifying, marking and counting the bone marrow cells acquired by the bone marrow cell morphology analyzer to form acquisition data and displaying the acquisition data; and an auditing end which is used for auditing, correcting and confirming the acquired data of the bone marrow smear scanning image acquired by the acquisition end, and generating a digital report. The system can automatically identify, mark and count the bone marrow smear, and the microscopic examination efficiency is greatly improved.

The invention discloses an application of Bcl-2 (B cell lymphoma)/IgH (immunoglobulin H) gene rearrangement used as B cell lymphoma bone marrow infiltration marks. The application provided by the invention is particularly an application of substances and/or instruments for detecting Bcl-2 gene and IgH gene rearrangement to the preparation of products for screening B cell lymphoma suspected bone marrow infiltration or potential bone marrow infiltration patients, and B cell lymphoma is diffuse large B cell lymphoma (DLBCL). Experiments prove that compared with the traditional single bone marrow smear morphology dyeing method, the morphology method combined with FISH (Fluorescence In Situ Hybridization) method has the advantage that the positive rate (DLBCL is 33.0 percent and is 10.3 percent) is higher than that of the single morphology dyeing method. The bone marrow smear FISH is used for detecting Bcl-2/IgH gene rearrangement, in addition, the bone marrow infiltration of 83.3 percent of cases with 1 to 5 percent of naive lymphocyte in the bone marrow smear morphology can be more early diagnosed to different degrees than the single morphology dyeing method, and the early warning for at least more than three months is realized by compared with the clinical recurrence. Powerful supplementation is provided for the clinical analysis, the treatment and the reexamination after the cure of the lymphoma bone marrow infiltration.

The invention discloses a bone marrow smear microscopic image analysis method and device, electronic equipment and a storage medium. The method comprises the following steps: acquiring a bone marrow smear microscopic image; inputting the bone marrow smear microscopic image into a bone marrow cell detection model to obtain a bone marrow cell detection result; wherein the bone marrow cell detectionmodel is used for predicting a position distribution diagram of bone marrow cells in the bone marrow smear microscopic image, fusing the position distribution diagram with an original feature map extracted from the bone marrow smear microscopic image to obtain fusion features, and outputting a bone marrow cell detection result according to the fusion features. The method does not depend on subjective judgment of medical personnel and has the advantages of being objective and efficient, prediction of the position distribution diagram is introduced into the bone marrow cell detection model, extracted semantic features are enriched, missing detection and wrong detection are not likely to happen, and the bone marrow cell detection accuracy is improved. Comprehensive analysis such as positioning, recognition, classification and statistics of bone marrow cells is further realized.

The invention discloses a digital intelligent acquisition system global box. The top cover of the global box and the bottom cover of the global box are fixedly connected to form a sealed acquisition area. A U-shaped bottom support plate is arranged on the bottom cover of the global box. A magnet block is arranged on the inner side corresponding to an opening of the bottom cover of the global box.Slots are arranged on three inner sides of the bottom cover and the bottom support plate of the global box. An open slot is arranged on the side of the bottom cover of the global box. A compression spring is arranged in the open slot. A global box spring plate is arranged on the compression spring. A slide support plate is pushed into the slots of the bottom cover and the bottom support plate of the global box. A camera module is fixed on the inner side of the top cover of the global box. The camera part of the camera module passes through the open slot of a light plate. A slide is fixed through a slide compression spring and a slide fixing point in X and Y axes. A bone marrow smear is placed in a closed space to capture an acquired image, which avoids natural light such as artificial illumination or reflection caused by a camera flash. The shooting sharpness is improved. When the global box is used to capture the acquired image, the global box has the advantages of high cell recognition and simple frame selection operation.

The invention relates to a method for judging the degree of myelohyperplasia, the method comprises the following steps: when a bone marrow smear reaches a specified position, starting a grey-spot camera, and automatically scanning the whole bone marrow smear by an electronic eyepiece; storing all the images of the bone marrow smear collected by the electronic eyepiece for later use; repeatedly collecting a large number of image samples of the bone marrow smear; dividing the collected picture samples according to the thickness and the uniformity of bone marrow cells, and generating an algorithm for calculating the thickness and the uniformity of the bone marrow smear according to the divided pictures; collecting a whole picture of the bone marrow smear again; calculating the thickness and the uniformity of the bone marrow smear by using an algorithm, and selecting a region with moderate thickness and uniform distribution of bone marrow cells in the bone marrow smear; respectively calculating the area of all mature red blood cells and the area of nucleated cells in the frame selection area, calculating the area ratio of the mature red blood cells to the nucleated cells, and judging the degree of myelohyperplasia according to the area ratio of the red blood cells to the nucleated cells in the bone marrow.

The invention discloses a mechanical arm for shooting bone marrow smears. The mechanical arm comprises an X-direction translation mechanism, a Y-direction translation mechanism, a Z-direction translation mechanism and a base, wherein the X-direction translation mechanism comprises an X-axis mounting plate, an X-direction translation assembly and an X-direction distance measurement assembly; the Y-direction translation mechanism comprises a connecting plate, a Y-axis mounting plate, a Y-direction translation assembly and a Y-direction distance measurement assembly; the Z-direction translation mechanism comprises a Z-axis mounting plate, a Z-direction translation assembly and a Z-direction distance measurement assembly; the X-axis mounting plate is mounted on the base; a connecting plate can slide on the X-axis mounting plate through the X-direction translation assembly; the Z-axis mounting plate can slide on the Y-axis mounting plate through the Y-direction translation assembly; and an electric clamping jaw is mounted on the Z-direction translation assembly and can slide on the Z-axis mounting plate through the Z-direction translation assembly. The invention also discloses a corresponding working method. The mechanical arm can be applied to rapid and accurate movement of bone marrow smears in the X-axis direction, the Y-axis direction and the Z-axis direction; and efficient and high-accuracy image acquisition under low load and low power consumption is achieved.