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Medulla smear plasmalogen staining agent group and use method

A technology of bone marrow smear and staining reagents, which is applied in the medical field, can solve the problems of decreased specificity of histochemical staining, insufficient basis for diagnosis of blood diseases, and low specificity, and achieves simple and easy staining methods, convenient observation, and specificity. strong effect

Inactive Publication Date: 2008-08-27
范洪 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specificity of the current histochemical staining of bone marrow smears is not strong, and the staining results of the same cell in different diseases are often different, or the staining results of different cells in the same disease are the same, which significantly reduces the specificity of histochemical staining , resulting in insufficient basis for the diagnosis of blood diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] First, prepare the bone marrow smear plasmalogen staining reagent group according to the following formula and method:

[0023] Liquid A: HgCl 2 Solution, its preparation method is: take HgCl 2 2 grams, add 100ml of water;

[0024] Solution B: Schiff reagent, its preparation method is: take 1 gram of basic fuchsin, dissolve it in 200ml of boiling water, filter it when it is cooled to 60°C, add 20ml of 1N hydrochloric acid, then add 2 grams of sodium bisulfite, and stopper. Leave to stand for 10 hours, then add 300mg of activated carbon, mix well, filter, the filtrate should be colorless and transparent, store at 6°C, the solution will be invalid when it turns red;

[0025] Liquid C: sulfurous acid aqueous solution, its preparation method is: take 5.5ml of 10% sodium bisulfite, 4.5ml of 1N hydrochloric acid, and 87ml of distilled water and mix evenly, and prepare it immediately before use;

[0026] Liquid D: Mayer's hematoxylin solution, its preparation method is: tak...

Embodiment 2

[0034] First, prepare the bone marrow smear plasmalogen staining reagent group according to the following formula and method:

[0035] Liquid A: HgCl 2 Solution, its preparation method is: take HgCl 2 1.8 grams, add 100ml of water;

[0036] Solution B: Schiff reagent, its preparation method is: take 0.9 g of basic fuchsin, dissolve it in 200 ml of boiling water, filter it when cooled to 58 °C, add 20 ml of 1N hydrochloric acid, add 1.8 g of sodium bisulfite, and stopper Keep it away from light for 8 hours, then add 300mg of activated carbon, mix well, filter, the filtrate should be colorless and transparent, store at 4°C, the solution will be invalid when it turns red;

[0037] Liquid C: sulfurous acid aqueous solution, its preparation method is: take 6ml of 10% sodium bisulfite, 5ml of 1N hydrochloric acid, and 90ml of distilled water and mix evenly, and prepare it immediately before use;

[0038] D solution: Mayer's hematoxylin solution, its preparation method is: take 0....

Embodiment 3

[0046] First, prepare the bone marrow smear plasmalogen staining reagent group according to the following formula and method:

[0047] Liquid A: HgCl 2 Solution, its preparation method is: take HgCl 2 2.2 grams, add 100ml of water;

[0048] Solution B: Schiff reagent, its preparation method is: take 1.1 grams of basic fuchsin, dissolve it in 200ml of boiling water, filter it when it is cooled to 62°C, add 20ml of 1N hydrochloric acid, then add 2.2 grams of sodium bisulfite, and stopper Keep it away from light for 12 hours, then add 300mg of activated carbon, mix well, filter, the filtrate should be colorless and transparent, store at 8°C, the solution will be invalid when it turns red;

[0049] Liquid C: sulfurous acid aqueous solution, its preparation method is: take 6.5ml of 10% sodium bisulfite, 5.5ml of 1N hydrochloric acid, and 91ml of distilled water and mix evenly, and prepare it immediately before use;

[0050] Liquid D: Mayer's hematoxylin solution, its preparation...

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Abstract

The invention belongs to the medical field, providing an acetal phosphatide dyeing reagent group for a bone marrow smear and an application method thereof, wherein the reagent group using HgCl2 as the fixture and the Schiff reagent as the dyeing solution includes following reagents: the HgCl2 solution, the Schiff reagent, the sulfite water solution and the Mayer brazilein solution; the steps of the application method are as follows: (1) the bone marrow smear is fixed by the HgCl2; (2)the bone marrow smear is washed by distilled water; (3) the bone marrow smear is dyed by the Schiff reagent and washed by the sulfite water solution; (4) the bone marrow smear is dyed again by the Mayer brazilein solution and washed by flowing water; (5) the judgment of the dyeing result is as follows: cytolymph which is red is positive, and cytolymph which is not dyed is negative. The dyeing method of the invention has the advantages of easy realization, convenient observation of the dyeing result and extremely high specificity, and is an effective method for judging megakaryocytes in the acute leukemia M7 and other leukemia cells.

Description

technical field [0001] The invention belongs to the medical field, in particular to a bone marrow smear plasmalogen staining reagent set and a use method. Background technique [0002] Due to the different pathological changes of various blood diseases, hematopoietic dysfunction and individual differences between patients, the same type of cells in different blood diseases, and the same type of cells between different individuals with the same disease, their morphology is easy to change, and it is even difficult to identify . Histochemical staining of bone marrow smear is the basic method to observe the morphological changes of hematopoietic cells, and is the main basis for the diagnosis and treatment of blood diseases. The reliability of bone marrow smear staining method is the key technology for correct diagnosis and effective treatment of blood diseases. Histochemical staining is specific staining for different cell systems of bone marrow, as well as the special structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/30
Inventor 范洪
Owner 范洪
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