32 results about "Boar sperm" patented technology

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.

The invention relates to a feed for promoting estrus of boars, which belongs to the technical field of production of feeds for pigs and is used for promoting the estrus of boars and improving the boar sperm quality. The feed for promoting the estrus of the boars and improving the boar sperm quality can be used for improving the reproduction performance of boars of child-bearing age. The feed for promoting the estrus of the boars consists of a conventional feed and Chinese herbal medicines and is characterized in that the conventional feed consists of a premix feed consisting of corns, bean pulp, imported fish meal, corn gluten meal, bran, wheat middling, soybean oil, calcium hydrophosphate, mountain flour, salt, lysine, choline chloride and composite vitamins for boars, a composite microelement premix feed and cysteamine; the Chinese herbal medicines include fenugreek grass, actinolite, prepared rehmannia root, cynomorium songaricum and longspur epimedium. According to the feed, on the basis of an optimal formula, the medicine property of the Chinese herbal medicines is sufficiently utilized, the reproduction organism functions of the boars are improved, the estrus of the boars is promoted, the sperm quality of the boars and the reproduction performance of the boars of child-bearing age are improved and the economic benefit is increased.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A/G and G/A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention discloses a diluent for in-vitro low temperature preservation of boar semen and a preparation method thereof. The diluent comprises following components: broad-spectrum antibiotic bacteriostatic agents of D-fructose, 2-amino-2-hydroxymethyl-1,3-propanediol, trisodium citrate, sodium bicarbonate, citric acid, disodium ethylene diamine tetraacetate, N-acetyl-cysteine, skim milk powder, yolk freeze-dried powder, amikacini sulfatis and the like. The diluent for the in-vitro low temperature preservation of the boar semen is suitable for preserving the fresh boar semen at low temperature (4-5 DEG C). The diluent significantly inhibits injury caused to sperm structure during the low temperature preservation process, maintains glucose metabolism and oxidative phosphorylation metabolism processes of boar sperm, effectively prolongs survival time of the boar sperm in the low temperature preservation, and significantly improves use efficiency of the high quality breeding boar semen and economic benefit of breeding.

The invention discloses a breeding boar composite premix for improving reproductive performance; the breeding boar composite premix is prepared from the following raw materials: corn, soybean meal, bran, fish meal, dicalcium phosphate, edible salt, korthalsella japonica, lotus seed, orobanche coerulescens, dioscorea alata, antler glue, gallnut, beautiful millettia root, semen euryales, prepared salt, elaeagnus angustifolia, dogwood, a compound probiotic, and a compound vitamin. The raw materials contain a variety of Chinese herbal medicines, the compatibility is scientific, the roles are complemented with each other, digestion and absorption of pigs on the feed can be promoted, the feed utilization rate and the total nutrient intake are improved, the boar reproductive body function can be improved, breeding boar estrus is regulated and promoted, the hybridization rate of breeding boars is increased, the quality of semen of the breeding boars is increased, and the parturition performance of the childbearing-age boars is increased; with addition of the compound vitamin, the number and vitality of boar sperms can be increased, and generation of sperm deformity is reduced, so that the survival rate and quality of piglets are ensured, and greater economic benefits are brought for raising households.

The invention provides a boar sperm preservative as well as a preparation method and application thereof, and belongs to the technical field of livestock breeding. The boar sperm preservative provided by the invention is prepared from glucose, sodium hydrogen carbonate, sodium citrate, EDTA, citric acid, trihydroxymethyl aminomethane, L-cysteine, levofloxacin, sodium carboxymethylcellulose, bovine serum albumin and Banqing toxin-removing oral liquid. Compared with a conventional diluent Zorlesco, the boar sperm preservative provided by the invention has a better preservation effect of boar sperms, can improve the antibacterial ability of boar semen, and prolongs the storage time of the boar sperms.

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to boar sperm motility and an application and an acquisition method thereof. Themolecular genetic marker related to boar sperm motility is located at the 8482542 bp position of chromosome 17 of pig, and is a mutation that C is greater than T; the molecular marker of the present application is obtained by single step genome-wide association study (wssGWAS); and the method is simple, efficient and fast.

The invention discloses boar fodder, being prepared from the following raw materials in parts by weight: 40 to 60 parts of corn flour, 1 to 3 parts of dangshen, 15 to 25 parts of barley, 1 to 3 parts of lysine, 6 to 10 parts of wheat, 1 to 3 parts of aloe, 15 to 25 parts of wheat bran, 2 to 4 parts of soya-bean oil, 5 to 7 parts of tofukasu, 1 to 3 parts of medlar, 2 to 4 parts of mountain flour, 2 to 4 parts of gingko leaves, 2 to 4 parts of bone meal, 6 to 8 parts of weed and 1 to 3 parts table salt. The boar fodder is reasonable in ratio, reduced in cost, rich in nutrition and capable of improving boar sperm motility.

The invention discloses a treatment method of boar semen and a use method of the treated boar semen, and belongs to the technical field of artificial insemination of boars, and the treatment method comprises the following steps: after collection, rapidly observing the color of original semen, measuring the pH value and measuring the volume; preserving the temperature of the diluent at 30-35 DEG C for 30-45 minutes, immediately putting the original semen into the diluent, sampling, and evaluating the sperm motility rate and the sperm density; performing isothermal dilution and uniform mixing on the diluent and the original semen, and filling semen bottles with the uniformly mixed diluted semen; and preserving in a dark place at 15-20 DEG C. The treatment method can supplement energy substances for the pig sperms, especially has the special functions of protecting the acrosome structure of the sperms, inhibiting sperm activity before insemination, prolonging the service life of the sperms and improving the insemination capacity, and has the tendentiousness effect of reducing the number of birth of boars and piglets. The method can be used for instant insemination or normal-temperature preservation. After the feed is used, the sperm quality of boars is improved, the conception rate of sows is increased, and the breeding cost is reduced.

The invention discloses a special feed for improving boar semen quality. The digestible energy of the special feed is 3100-3200 kcal/kg, the digestible lysine content is 0.75-0.8%, and the protein level is 15.5-16.5%. Research finds that: the digestive energy level has very significant influence on the number of effective sperms. Protein concentration is related to sperm motility rate and is affected by the protein level. The digestive energy level has a significant influence on the sperm malformation rate, and the protein level also has a significant influence on the malformation rate. The special material of the invention has a remarkable effect on improving the sperm motility, the malformation rate, and the seminal fluid volume and density of boar.

The invention provides a pig sperm malformation rate related molecular genetic marker. The molecular genetic marker site is H3GA0014627, wherein the H3GA0014627 marker site is the 123567821th bp position of the No.4 chromosome of boar, the 123567821th bp position is a mutation site with C being greater than T, and the pig reference genome is Sscrofa11.1. The molecular genetic marker is used for marking boars of different genotypes. The sperm malformation rate has significant difference, T allele significantly reduces the sperm malformation rate, can assist the breeding of boars by detecting the genotype of the H3GA0014627 molecular genetic marker site, selects homozygous boars with the low sperm malformation rate, and reduces the sperm malformation rate, thereby effectively improves the utilization efficiency of the boars.

A method for preparing an agonist for improving boar sperm motility includes: (1) collecting and infiltrating a testicular tissue of a young boar 3-5 days after born; (2) washing the tissue with PBS; (3) incubating the tissue in a cell culture medium, and centrifuging the cell suspension to remove a supernatant; (4) adding hyaluronidase and collagenase IV followed by shaking, and adding the cell culture medium and centrifuging to remove a supernatant; (5) adding trypsin and deoxyribonuclease followed by shaking, and adding the cell culture medium; (6) filtering the cell suspension by a cell sieve; (7) centrifuging to remove a supernatant, and adding the cell culture medium; (8) culturing and passaging the cells; (9) culturing and storing the cell suspension. The invention has the advantages of good application effect, low cost and simple production process.

The invention provides a sperm collecting table for boars. The sperm collecting table is characterized in that sperm collecting fences (2) are arranged on a lifting table (1), a boar driving channel (3) is hinged to an entrance of the sperm collecting fences and is vertically rotationally hinged to the sperm collecting fences, and pulleys (4) are arranged at contact points of the boar driving channel and the ground. The sperm collecting table has the advantages that the problem that sperm collecting workers often need to squat and stoop down for collecting sperm of boars in boar sperm collecting procedures, are easy to get tired due to long-term squatting and stooping actions and are easy to suffer from occupational diseases can be solved; the lifting table is arranged at the bottoms of the sperm collecting fences, the heights of the sperm collecting fences can be adjusted by the aid of the lifting table, accordingly, workers can collect sperm of the boars while standing, the labor intensity can be effectively relieved for the sperm collecting workers, and shortcomings due to the traditional sperm collecting modes can be overcome.

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to linear motion of boar sperm, and an application and a method thereof. The method analyzes the linear motion traits of boar sperm by genome-wide association study (wssGWAS) analysis, and obtains three molecular genetic markers related to linear motion of boar sperm, which are located at the 136112947 bp position of chromosome 15 of pig, and is a mutation that T is greater than C; at the 18505448 bp position of chromosome 3 of pig, and is a mutation that A is greater than G;and at the 63272581 bp position of chromosome 11 of pig, and is a mutation that C is greater than T. The method used to analyze the linear motion of boar sperm is simple, efficient and fast.