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A molecular marker associated with boar sperm concentration traits

A molecular marker, boar technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of increasing the cost of raising boars, etc., and achieve the effect of high sensitivity and good specificity

Active Publication Date: 2021-05-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, poor semen quality is one of the main reasons for boar culling, and the replacement ratio of boars due to semen quality problems is as high as 10-30% (Robinson J A B, Buhr M M. Impact of genetic selection on management of boar replacement [J ].Theriogenology, 2005,63(2):0-678.), the boar replacement of abnormal factors leads to the increase of boar feeding cost; on the other hand, more qualified semen produced by boars can produce more artificial insemination stations. high economic return

Method used

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  • A molecular marker associated with boar sperm concentration traits
  • A molecular marker associated with boar sperm concentration traits
  • A molecular marker associated with boar sperm concentration traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Genotyping Detection

[0028] (1) Using the magnetic bead method sperm genome extraction kit (purchased from Wuhan Nanomagnetic Biotechnology Co., Ltd.) to automatically extract the total DNA of Duroc boar sperm, the specific steps are:

[0029] (1) Take an appropriate amount of semen (5-15 μL) from a Duroc boar into a 1.5 mL centrifuge tube;

[0030] (2) Add 500 μL of lysate (included with the above kit) and 5 μL of proteinase K (20 mg / mL) to the centrifuge tube, shake and mix for 30 seconds, place in a 65°C oven or metal bath, and lyse for 30 minutes to 1 Hour;

[0031] (3) After the lysis is complete, transfer all the supernatant to a deep-well plate (marked as ①), and add 350 μL of isopropanol to each well;

[0032] (4) Put the No. ① deep-well plate on station 1 of the nucleic acid extractor, and place the deep-well plates with magnetic beads, washing solution ①, washing solutions ② and ③ and eluent on station 2~ 6 on. Turn on the power of the instrum...

Embodiment 2

[0038] Example 2 Application of WU_10.2_17_4574599 Molecular Marker Typing Method in Association Analysis of Duroc Boar Semen Traits

[0039] (1) Phenotypic pretreatment of semen traits of Duroc boars

[0040] The traits of boar semen belong to repeated measurement traits. It is necessary to use the mixed linear model (MLM). The joint effect of boar station, year and month is used as the fixed effect, the age in days and semen collection interval are used as covariates, and the individual is used as a random effect to calculate the individual random effect. Effects, as newly constructed phenotypes (pseudo-phenotypes) for subsequent GWAS analysis. The lme4 package in the R statistical environment was used for analysis. The data included 2020 individuals and 105201 semen collection records. The specific model is as follows:

[0041]

[0042] Among them, y ijklm is the original phenotypic value of semen traits (sperm concentration) of the lth individual at the mth time; μ i...

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Abstract

The invention belongs to the technical field of pig molecular marker screening, and in particular relates to a molecular marker associated with boar sperm concentration traits. The molecular marker is cloned from a gene fragment with accession number WU_10.2_17_4574599, and the gene is typed by gene chip technology , screened to obtain a molecular marker associated with pig sperm concentration traits, its nucleotide sequence is shown in SEQ ID NO: 1, there is a T / C allelic mutation at the 101st base of the sequence , when the 101st nucleotide of SEQ ID NO: 1 is C, it is determined that the pig has a higher sperm concentration. The invention provides a new SNP molecular marker resource for marker-assisted selection of pig semen traits.

Description

technical field [0001] The invention relates to the technical field of preparation of pig molecular markers, in particular to a molecular marker associated with boar sperm concentration traits. The molecular marker described in the invention can be used for marker-assisted selection of boar sperm concentration traits. Background technique [0002] Large-scale and intensive pig raising is the main development trend of my country's pig farming model. Since the successful application of pig artificial insemination technology in Guangxi in the 1950s, pig artificial insemination technology has been widely promoted throughout the country. At present, in large-scale pig farms, the use of artificial insemination technology has reached more than 85%. The reason is that pig artificial insemination technology has the following advantages: (1) reduce the number of boars and reduce the cost of boars; (2) facilitate the implementation of off-site breeding and quickly improve the genetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 李新云陈毅龙赵书红项韬施亮赵云翔张从林刘小磊梅全顺刘阿丽
Owner HUAZHONG AGRI UNIV
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