30 results about "Aeromonas sp" patented technology

The invention discloses a method for efficiently degrading azo dye in dye wastewater by using bacteria. The method comprises the following specific steps: (1) inoculating a strain Aeromonas sp.DH-6 into an LB (Luria-Bertani) culture medium, culturing over night in a shaker in the rotation speed of 150-200rmp at 25-35 DEG C, so as to obtain a great amount of bacteria; (2) inoculating the over-night culture which is 2-8% in inoculation amount into the dye wastewater containing azo dye, and standing to culture and decolor, wherein the decoloring pH value is 3.0-7.0, the temperature is 5-45 DEG C, and the decoloring culture time is more than 4 hours. The method disclosed by the invention is still high in decoloring rate for methyl orange under acid conditions at relatively high temperature (about 40 DEG C). Besides, no remarkable influence is caused by most meal ions in coloring, the decoloring rate can be still approximate to 90% when 1000mg / L of the dye is cultured for 48 hours by using the bacteria, and thus the decoloring effect is relatively good. Therefore, compared with a conventional method, the bacterium DH-6 can be used for more efficiently degrading the azo dye with low energy consumption, and is higher in environment applicability, free of secondary pollution to the environment, and wider in research value and application prospect.

The invention discloses algae-lysing aeromonas sp. and an application thereof in controlling cyanobacterial blooms. The aeromonas sp. GLY-2107, which has a significant algae-lysing activity, is separated from a water body from Lake Taihu, and the preservation number of the aeromonas sp. is CGMCC No.8979; and active algae-lysing ingredients, namely 3-phenmethyl-piperazine-2,5-dione and 3-methylindole, are separated, purified and identified from a metabolic product of the aeromonas sp., wherein the median lethal dose LD50 of the 3-phenmethyl-piperazine-2,5-dione on microcystis aeruginosa 9110 is 4.72 [mu]g / mL and the median lethal dose LD50 of the 3-methylindole on the microcystis aeruginosa 9110 is 1.10 [mu]g / mL. The algae-lysing aeromonas sp. is applicable to research, development and production of novel biological algicides, and is finally applied to the control of the cyanobacterial blooms in lakes.

The invention provides a denitrification bactericide. The bactericide comprises Aeromonas sp. PR-DN1, Alcaligenes sp. PR-DN2, Acinetobacter sp. PR-DN3, Bacillus cereus. PR-DN4 and Exiguobacterium sp.PR-DN5. The bactericide can be used for wastewater treatment after being put into wastewater containing nitrate nitrogen and nitrite nitrogen to be evenly mixed. The denitrification bactericide is anefficient bactericide which is greatly screened and compounded according to the characteristics of the wastewater, the nitrate nitrogen and nitrite nitrogen in the wastewater can be effectively removed, operation is simple, the treatment cycle is shortened, and the denitrification bactericide is friendly to the environment and low in operation cost.

The invention relates to a method of removing bloom-forming cyanobacteria by using Aeromonas sp., wherein, the strain of Aeromonas sp. DS-1 has been deposited in China Center for Type Culture Collection (CCTCC) on May 23rd, 2011 and assigned the accession number CCTCC No.M2011180. According to the invention, cells of the strain of Aeromonas sp. DS-1 are added into a water sample containing 106 to107 cells of cyanobacteria per milliliter at a temperature of 20 to 35 DEG C with a pH value being 6 to 7.5, and an obtained uniformly mixed mixture is allowed to stand and is subjected to treatment for 4 to 10 days; the method has an obvious and thorough effect in removing three representative cyanobacteria with great harm at present, i.e., Microcystis aeruginosa, Anabaena and Nodularia, and canbe used for controlling bloom-formation of cyanobacteria in real water bodies.

The invention belongs to the technical field of biological enzymes, and in particular relates to a pullulanase, a pullulanase producing strain and application of the pullulanase. The pullulanase is derived from Aeromonas sp. As in bacteria. The pullulanase produced by the strain is pullulanase type I, and can specifically hydrolyze alpha-1,6 glycosidic bond in pullulan or starch. The pullulanase is expressed under an induction condition in which 1.5% soluble starch is used as a substrate. Under a condition of medium pH of 7.5 and culture temperature of 30 DEG C, shake-flask fermentation is carried out for 48h to produce the pullulanase at a level up to 24.8U / mL. For the pullulanase produced by the strain, the optimum reaction temperature is 60 DEG C, the optimum pH is 6.0, and the relative enzyme activity in the pH range of 6.0-9.0 is greater than 90 percent. After treatment for 50min at 60 DEG C, the residual activity of the pullulanase reaches about 50 percent. The pullulanase type I provided by the invention can be applied to industries such as starch processing, environment protection, foods, medical care and the like.

The invention belongs to the technical field of biological enzymes, and in particular relates to a pullulanase, a pullulanase producing strain and application of the pullulanase. The pullulanase is derived from Aeromonas sp. As in bacteria. The pullulanase produced by the strain is pullulanase type I, and can specifically hydrolyze alpha-1,6 glycosidic bond in pullulan or starch. The pullulanase is expressed under an induction condition in which 1.5% soluble starch is used as a substrate. Under a condition of medium pH of 7.5 and culture temperature of 30 DEG C, shake-flask fermentation is carried out for 48h to produce the pullulanase at a level up to 24.8U / mL. For the pullulanase produced by the strain, the optimum reaction temperature is 60 DEG C, the optimum pH is 6.0, and the relative enzyme activity in the pH range of 6.0-9.0 is greater than 90 percent. After treatment for 50min at 60 DEG C, the residual activity of the pullulanase reaches about 50 percent. The pullulanase type I provided by the invention can be applied to industries such as starch processing, environment protection, foods, medical care and the like.

The invention discloses a method for preparing alga oligosaccharide with anticoagulation activity. Specifically, in the method, an alga oligosaccharide sample prepared from sargassum, gelidium amansii or green laver is hydrolyzed with an enzyme so as to obtain the alga oligosaccharide with anticoagulation activity, wherein the enzyme is produced by inducing an ocean strain of Pseudomonas vesicularis and / or Aeromonas salmonicida with the ocean polysaccharide from sargassum, gelidium amansii or green laver.

The invention relates to a method of removing bloom-forming cyanobacteria by using Aeromonas sp., wherein, the strain of Aeromonas sp. DS-1 has been deposited in China Center for Type Culture Collection (CCTCC) on May 23rd, 2011 and assigned the accession number CCTCC No.M2011180. According to the invention, cells of the strain of Aeromonas sp. DS-1 are added into a water sample containing 106 to 107 cells of cyanobacteria per milliliter at a temperature of 20 to 35 DEG C with a pH value being 6 to 7.5, and an obtained uniformly mixed mixture is allowed to stand and is subjected to treatment for 4 to 10 days; the method has an obvious and thorough effect in removing three representative cyanobacteria with great harm at present, i.e., Microcystis aeruginosa, Anabaena and Nodularia, and can be used for controlling bloom-formation of cyanobacteria in real water bodies.

The invention provides a denitrification bacterial agent, which comprises Aeromonas ( Aeromonas sp. ) PR‑DN1, Alcaligenes ( Alcaligenes sp. ) PR‑DN2, Acinetobacter ( Acinetobacter sp. ) PR‑DN3, Bacillus cereus ( Bacillus cereus. ) PR‑DN4, Microbacteria ( Exiguobacterium sp. ) PR‑DN5, put the above bacterial agent into wastewater containing nitrate nitrogen and nitrite nitrogen, mix it evenly, and then it can be used for wastewater treatment; the nitrogen removal bacterial agent described in the present invention is based on the characteristics of wastewater, after a large The high-efficiency bacterial agent obtained by screening and compounding can effectively remove nitrate nitrogen and nitrite nitrogen in wastewater, is simple to operate, shortens the treatment cycle, is environmentally friendly, and has low operating costs.

The invention provides a simple method for preparing high-purity L-amino acid by biocatalysis. A viable cell of bacteria Aeromonas sp. (preserving number CGMCC 2226) containing a plurality of enzymes can selectively oxidize D-type isomer in racemate amino acid under the condition of aerating air to leave unreacted high-purity L-amino acid. The method is reasonable in design without common derivative steps of protection in advance and protection once again in the prior method, is capable of directly and selectively transforming the D-type amino acid in the racemate, does not use metal catalysts, organic solvent or accessory ingredients during preparing process, and carries out reactions in neutral condition without chemical discharge except reproducible biomass, thereby belonging to green and environment-friendly production process. The method has the advantages of simple and short route, high efficiency, resource conservation and simple steps. The method is applicable to elimination of D-type antipode in more amino acids in different types and various mixtures possibly formed by the amino acids.

The invention relates to an aerobic denitrifying bacterium strain and application thereof. The aerobic denitrifying bacterium strain is Aeromonas sp. AD-3, and is collected by China General Microbiological Culture Collection Center. The collection address is Beichenxi Road No.1 Yard 3#, Chaoyang District, Beijing City; the collection date is July 14th, 2015; and the collection number is CGMCC No.11069. The Aeromonas sp. AD-3 is used for treating nitrogenous wastewater. The gene of the Aeromonas sp. AD-3 has higher aerobic denitrifying property. The total nitrogen removal rate is up to 87.7%, and thus, the Aeromonas sp. AD-3 has significant meanings in the field of water treatment due to the characteristics.

The invention discloses a microbial Aeromonas strain that can be used for cocoon silk degumming. The Latin literary name is Aeromonas sp. CCTCC NO: M2017192, this strain is screened from cocoon silk decay liquid. The present invention further provides an application method for directly acting on the cocoon silk raw material to effectively remove the sericin in the cocoon silk. Through the steps of degumming microbial preparation, cocoon silk microbial degumming treatment, washing and drying steps, the cocoon silk can be effectively removed. Sericin can further reduce the use of chemical materials in the degumming process of silk spinning raw materials, improve the working environment and reduce environmental pollution.

The invention discloses an efficient denitrification aerobic denitrifying bacterium and application thereof, and relates to the technical field of environmental microorganisms and water treatment. The bacterial strain is named as Aeromonas sp.RC-15, and the bacterial strain is named as Aeromonas sp.RC-15. The efficient denitrification aerobic denitrifying bacterium is obtained through screening and separation, the nitrate nitrogen removal rate reaches 95.57% under the optimum condition within 24 h, the corresponding nitrate nitrogen removal rate reaches 14.62 mg / h <-1 >, and the efficient denitrification aerobic denitrifying bacterium has the characteristics of low temperature resistance, low COD and the like, has a certain adsorption and biofilm formation effect when river sand is used as a carrier, and has the advantages that the efficient denitrification aerobic denitrifying bacterium can be used for treating tail water of an actual sewage treatment plant. The denitrification rate of a system in which the Aeromonas sp.RC-15 is added is obviously improved compared with that of a system in which the strain is not added, the total nitrogen removal rate and the nitrate nitrogen removal rate of a blank group are improved by 25%-35% and 10%-20% respectively, and the total nitrogen removal rate and the nitrate nitrogen removal rate can reach 79.0% and 90.0% at most; the strain has a wide application prospect in the processes of low-concentration nitrate wastewater treatment, artificial wetland ecological restoration and the like.

The invention discloses a method for efficiently degrading azo dye in dye wastewater by using bacteria. The method comprises the following specific steps: (1) inoculating a strain Aeromonas sp.DH-6 into an LB (Luria-Bertani) culture medium, culturing over night in a shaker in the rotation speed of 150-200rmp at 25-35 DEG C, so as to obtain a great amount of bacteria; (2) inoculating the over-night culture which is 2-8% in inoculation amount into the dye wastewater containing azo dye, and standing to culture and decolor, wherein the decoloring pH value is 3.0-7.0, the temperature is 5-45 DEG C, and the decoloring culture time is more than 4 hours. The method disclosed by the invention is still high in decoloring rate for methyl orange under acid conditions at relatively high temperature (about 40 DEG C). Besides, no remarkable influence is caused by most meal ions in coloring, the decoloring rate can be still approximate to 90% when 1000mg / L of the dye is cultured for 48 hours by using the bacteria, and thus the decoloring effect is relatively good. Therefore, compared with a conventional method, the bacterium DH-6 can be used for more efficiently degrading the azo dye with low energy consumption, and is higher in environment applicability, free of secondary pollution to the environment, and wider in research value and application prospect.

The invention discloses aeromonas sp. YQ and its application in the enzymatic preparation of L-citrulline. The aeromonas sp. YQ is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan, China under CCTCC No: M2015271 on 3th May, 2015, with postcode being 430072. The aeromonas sp. YQ obtained by screening is able to convert the substrate L-arginine to produce L-citrulline; whole-cells of the aeromonas sp. YQ are able to act for 3 days in pH 6 acetate buffer at 30 to 37 DEG C at a speed of 180r / min and able to convert 100g / L of the substrate L-arginine to produce 75g / L of L-citrulline, a product is about 47.8% in purity, the whole process has a total product yield of 40%, and basis is laid for industrialization of the enzymatic production of L-citrulline.

The invention provides aeromonas (Aeromonas sp.) 6-38. The aeromonas is separated from frozen livestock and poultry sewage and has a collection number of CGMCC No. 4402. The strain can be used for effectively decomposing starch, protein and fat simultaneously, is prepared into a microbial agent under a low-temperature environment and is used in livestock and poultry excrement compost, so that temperature rise of the compost can be promoted; and the microbial agent has a simple preparation process and a short fermentation period and is easy to store.