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Efficient denitrification aerobic denitrification strain and application thereof

A technology for aerobic denitrification and nitrification strains is applied in the field of high-efficiency denitrification aerobic denitrification bacteria, which can solve the problems of poor film-forming efficiency, difficult for microorganisms to survive, and a large amount of carbon sources.

Pending Publication Date: 2022-07-29
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, studies have shown that the survival temperature of most denitrifying bacteria is 27-37°C, which requires a large amount of carbon sources, and the efficiency of film formation is poor.
At present, the actual water temperature of the tail water of the sewage plant is only 10-20 ℃, and it will be lower in autumn and winter. The COD is only 100 mg / L or even lower, and most microorganisms are difficult to survive.

Method used

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  • Efficient denitrification aerobic denitrification strain and application thereof
  • Efficient denitrification aerobic denitrification strain and application thereof
  • Efficient denitrification aerobic denitrification strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening of aerobic denitrifying strains

[0030] Specific steps are as follows:

[0031] Preparation medium: 1) Screening separation medium: sodium acetate 2 g, tryptone 15 g, yeast extract 3 g, glucose 1 g, NaCl 6 g, KNO 3 1.5 g. 2) Denitrification medium (DM): CH 3 COONa 2.83g, KH 2 PO 4 0.40 g, KNO 3 1.0—4.0 g, Tris-HCl solution 12.0 mL, MgCl 2 0.20g, CaCl 2 0.09 g, trace elements 2.0 mL. The solid medium of the above-mentioned two kinds of culture medium needs to add 20 g agar powder again, add distilled water to dilute to 1000mL, pH is 7.0 ± 0.5. .

[0032] Take 1 mL of rhizosphere soil supernatant, add it to DM liquid medium, and carry out enrichment culture for 5-6 times at 30 °C and 160 r / min until gas is rapidly generated on the surface of the culture medium. Afterwards, 100 μL of bacterial liquid was inoculated onto the screening and isolation solid medium, incubated upside down at 30 °C for 36 h, and then streaked on multiple plates, and the ...

Embodiment 2

[0038] Denitrogenation experiments of strains under optimum conditions (temperature 30℃, pH 8 and C / N 7)

[0039] The specific implementation steps are as follows:

[0040] The strains were inoculated on the screening and separation liquid medium in advance for activation, and were shaken at 30 °C and 160 r / min for 36 h. After culturing, 50 mL of bacterial solution was taken and centrifuged at 8000 × g for 5 min at 4 °C. The supernatant was discarded, and the bacterial solution was resuspended after washing three times with sterile water. The OD value of the strain at 600 nm was 1.0 ± 0.05. , and then inoculate the bacterial solution in 200 mL of DM liquid medium containing different components according to 5% of the volume ratio of the culture solution to explore the optimization of the optimal environmental factors (temperature 30 °C, pH 8 and C / N 7). The denitrification ability of the strain.

[0041] The calculation method for the removal rate of nitrate and total nitrog...

Embodiment 3

[0044] Strain denitrification experiment at 15℃ and COD of 98mg / L

[0045] The specific implementation steps are as follows:

[0046] The simulated wastewater is configured in combination with the water quality indicators of the actual wastewater. The water quality indicators of the actual wastewater and simulated wastewater are shown in Table 1. Under the simulated wastewater conditions, the Figure 4 As shown, the removal rates of total nitrogen and nitrate nitrogen by strain RC-15 were 83.37% and 73.89%, respectively, and the concentration of COD decreased from 98.14 mg / L to 22.70 mg / L after 48 h. It can be seen that the strain can also perform good denitrification at low temperature and low COD, and has the prospect of being applied to practical wastewater.

[0047] Table 1 Wastewater indicators

[0048]

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Abstract

The invention discloses an efficient denitrification aerobic denitrifying bacterium and application thereof, and relates to the technical field of environmental microorganisms and water treatment. The bacterial strain is named as Aeromonas sp.RC-15, and the bacterial strain is named as Aeromonas sp.RC-15. The efficient denitrification aerobic denitrifying bacterium is obtained through screening and separation, the nitrate nitrogen removal rate reaches 95.57% under the optimum condition within 24 h, the corresponding nitrate nitrogen removal rate reaches 14.62 mg / h <-1 >, and the efficient denitrification aerobic denitrifying bacterium has the characteristics of low temperature resistance, low COD and the like, has a certain adsorption and biofilm formation effect when river sand is used as a carrier, and has the advantages that the efficient denitrification aerobic denitrifying bacterium can be used for treating tail water of an actual sewage treatment plant. The denitrification rate of a system in which the Aeromonas sp.RC-15 is added is obviously improved compared with that of a system in which the strain is not added, the total nitrogen removal rate and the nitrate nitrogen removal rate of a blank group are improved by 25%-35% and 10%-20% respectively, and the total nitrogen removal rate and the nitrate nitrogen removal rate can reach 79.0% and 90.0% at most; the strain has a wide application prospect in the processes of low-concentration nitrate wastewater treatment, artificial wetland ecological restoration and the like.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms and water treatment, in particular to a high-efficiency denitrifying aerobic denitrifying bacterium and its application. Background technique [0002] Nitrogen is one of the important pollutants leading to water pollution, which can cause water quality deterioration, eutrophication of water bodies, and then lead to the death of aquatic animals. Therefore, the treatment of nitrogen has been paid more and more attention, and the biological denitrification technology has attracted the attention of the majority of researchers. Biological denitrification is considered to be the most promising wastewater denitrification method due to its safety, efficiency and economy. Traditional biochemical denitrification goes through two processes: aerobic nitrification and anaerobic denitrification. First, ammonia nitrogen is converted into nitrate nitrogen by aerobic nitrifying bacteria, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01C02F101/16
CPCC02F3/02C02F3/34C02F2101/163
Inventor 杨本芹朱红旭潘学军赵艳卿高媛
Owner KUNMING UNIV OF SCI & TECH
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