Aeromonas 6-38 and application thereof

A technology for Aeromonas and strains, which is applied to Aeromonas 6-38 and its application in low-temperature solid waste composting treatment, can solve the problems of prolonging the decomposition cycle of organic wastes and reducing composting efficiency, etc.

Active Publication Date: 2013-03-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some low temperature areas or low temperature seasons in spring and winter, because the low temperature seriously affects the metabolic activities of microorganisms, the decomposition cycle of organic waste is prolonged and the efficiency of composting is reduced.

Method used

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  • Aeromonas 6-38 and application thereof
  • Aeromonas 6-38 and application thereof
  • Aeromonas 6-38 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 16-38

[0027] The identification of embodiment 16-38 bacterial strain

[0028] 1.1 Physiological and biochemical assays

[0029] The isolated bacterial strain (preservation number is CGMCC No.4402) is identified, and the method of physiological and biochemical determination is according to the standard method (Bacterial Taxonomy Group, Institute of Microbiology, Chinese Academy of Sciences, 1978; Dong Xiuzhu, Cai Miaoying, etc., 2001), part of the physiological and biochemical The traits are shown in Table 1.

[0030] Table 1 Physiological and biochemical identification of bacterial strains

[0031]

[0032] 1.216S rDNA sequence analysis

[0033] After inoculating strain 6-38 in liquid LB medium and shaking culture for 24 hours, take 1 mL of the bacterial liquid in a 1.5 mL centrifuge tube, and collect the bacterial cells after centrifugation at 12000 r / min for 3 min;

[0034] Add 567 μL of TE buffer to the cells and shake to fully suspend the cells; add 30 μL of 10% SDS, 3 μL ...

Embodiment 2

[0048] 1) Slant culture: inoculate 6-38 original strains on LB solid medium under sterile conditions, and cultivate at 25°C for 2-6 days to activate the strains;

[0049] 2) First-level seed cultivation: inoculate 10% of the bacteria cultured in step 1) into 5ml of LB liquid medium by volume ratio under aseptic conditions, and culture them on a shaker at 100-200r / min for 2-6 hours at 25°C. days, single strain liquid culture OD 600 When the value is between 1.5-2.4, the cultivation is stopped, and the first-grade seeds are obtained;

[0050] 3) Secondary seed culture: Inoculate the primary seeds in 1L of LB culture medium at an inoculum volume of 5-20% according to the volume ratio of the liquid medium, and inoculate them on a shaker at 100-200r / min at 25°C Cultivate for 2 to 6 days, and the bacterial concentration reaches 10 8 -10 9 cfu / mL, mixed with the matrix corn flour at a ratio of 600g / L to obtain the bacterial agent.

Embodiment 3

[0052] The inoculum prepared in Example 2 was added to the compost system, and the inoculum prepared by the mesophilic strain Bacillus megaterium (1.2393) (purchased from China Common Microorganism Culture Collection Center) was used as a control, and chicken manure and sawdust were used as compost Material, adjust the C / N ratio to 28, moisture 60%, compost at room temperature of 13-15°C, measure the temperature change of the compost every 5h, the results are shown in Table 1, the results show that the 6-38 do The temperature of the inoculant pile reached 39.6°C at 28 hours, and the temperature of the pile made of the inoculum by the mesophilic strain Bacillus megaterium reached 30.1°C.

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Abstract

The invention provides aeromonas (Aeromonas sp.) 6-38. The aeromonas is separated from frozen livestock and poultry sewage and has a collection number of CGMCC No. 4402. The strain can be used for effectively decomposing starch, protein and fat simultaneously, is prepared into a microbial agent under a low-temperature environment and is used in livestock and poultry excrement compost, so that temperature rise of the compost can be promoted; and the microbial agent has a simple preparation process and a short fermentation period and is easy to store.

Description

technical field [0001] The invention relates to a new microorganism strain and its application, in particular to an Aeromonas 6-38 and its application in low-temperature solid waste composting treatment. Background technique [0002] With the increasing amount of organic solid waste, the role of composting technology is also increasing. Composting technology is actually a process in which microorganisms decompose and utilize organic matter to make it mature, and microorganisms are the main actors in this process. The catabolism of microorganisms is affected by environmental factors such as ambient temperature, nutrient composition, and pH. Only in a suitable environment can they carry out active metabolic activities and decompose and utilize organic waste. However, in some low-temperature areas or low-temperature seasons in spring and winter, the low temperature seriously affects the metabolic activities of microorganisms, which leads to a prolonged decomposition cycle of o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/00C05F9/04C12R1/01
CPCY02A40/20Y02W30/40
Inventor 李季王莉瑛
Owner CHINA AGRI UNIV
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