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Aeromonas strain and application thereof

A technology for Aeromonas and strains, which is applied to Aeromonas strains and their application fields, can solve the problems of limited treatment application, weak salt or acid resistance, and poor broad-spectrum, and achieves high degradation effect and degradation. Broad spectrum effect

Active Publication Date: 2015-06-03
浙江至美环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the above-mentioned strains have poor broad-spectrum, can only degrade and decolorize one dye or a type of dye, and are not strong in salt or acid resistance, and are still very limited in the application of dye wastewater treatment

Method used

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  • Aeromonas strain and application thereof
  • Aeromonas strain and application thereof
  • Aeromonas strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Aeromonas (Aeromonas sp.) Isolation of CM-T1

[0027] Aeromonas sp. CM-T1 was isolated from soil collected from a sewage outlet of a printing and dyeing factory in Shaoxing. The specific separation method is: take 5.0g of soil and add it to a 250mL sterilized Erlenmeyer flask containing 4 glass beads, add 95mL of sterilized physiological saline, and vibrate on a shaker at 150r / min for 3h; take the above bacterial suspension, press 2% ( The amount of v / v) was added to the 150mL rich medium (KH 2 PO 4 1.8g / L, Na 2 HPO 4 12H 2 O 3.5g / L, MgSO 4 ·7H 2 O 0.29g / L, FeCl 3 ·6H 2 O 0.01g / L, NaCl 3.0g / L, peptone 10.0g / L, beef extract 3.0g / L, pH 7.0~7.2) in a serum bottle, cultured at 30°C and 150r / min for 3 days, and the Dilution to 10 -5 , 10 -6 , 10 -7 , take 0.1mL of the diluted solution and spread it on crystal violet dye solid medium (beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, crystal violet 20mg / L, agar 15g / L, pH 7.0~ In 7.2), two parallels...

Embodiment 2

[0028] Example 2 Identification of Aeromonas (Aeromonas sp.) CM-T1

[0029] (1) Morphological characteristics

[0030] Aeromonas sp. (Aeromonas sp.) CM-T1 was cultured on solid growth medium for 48 hours, the colony was yellow, the surface was protruding, moist and shiny, and the edges were neat; electron microscope scanning showed that the cells of the strain were brevibacteria, with two ends Microcircle, with a diameter of about 1.3μm, such as figure 1 , figure 2 shown.

[0031] (2) Physiological and biochemical identification

[0032] Biochemical Indicators

result

Biochemical Indicators

result

Catalase

+

Oxidase

+

glucose fermentation

+

Sorbitol Fermentation

-

Mannitol Fermentation

+

Salicin fermentation

+

MR

-

V-P

+

Nitrate reduction

-

Urease

-

arginine dihydrolase

+

Ornithine decarboxylase

-

Lysine decarboxylase

-

Tr...

Embodiment 3

[0047] The best growth condition optimization of embodiment 3 Aeromonas (Aeromonas sp.) CM-T1

[0048] Strain culture medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, pH 7~7.2

[0049] (1) Optimum growth pH of the inventive bacterial strain

[0050] Adjust the initial pH of the culture medium to 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0 with 1.0 mol / L HCl and NaOH, respectively, insert 0.5% inoculum into CM-T1, 30°C, Cultivate at 150r / min, and after 20h, use a UV-visible spectrophotometer at OD 600 The absorbance was measured, and the culture medium without inoculation was used as the blank control.

[0051] Depend on Figure 4 It can be seen that CM-T1 grows well when the pH is between 5 and 10, and the growth range of pH is wide, but the optimum growth pH is 7.0.

[0052] (2) Optimum growth temperature of the invented strain

[0053] Inoculate CM-T1 with 0.5% (v / v) inoculum in the culture medium, place them in shaker incubators at 20°C, 25°C, 30°C, 35°C, 40°C, a...

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Abstract

The invention discloses an aeromonas strain and application thereof and belongs to the field of environmental microorganism application. The aeromonas strain is named as (aeromonas sp.)CM-T1 and is preserved in China Center for Type Culture Collection (CCTCC for short) with the preservation number of CCTCC NO:M2014328 and the preservation date of July 9th, 2014. The aeromonas strain has the advantages that culture conditions are simple, the environment adaptability is strong, the dye degradation spectrum is wide, and dyes can be efficiently degraded, so that the aeromonas strain can be applied to the fields of printing and dyeing wastewater, dye wastewater, papermaking wastewater and tannery wastewater.

Description

technical field [0001] The invention relates to the application field of environmental microorganisms, in particular to an Aeromonas strain and its application. Background technique [0002] The pollution of dye wastewater to the environment is extremely serious. With the change of time and the development of society, human production and life style have become more and more advanced, and human's wanton plundering and destruction of nature and irresponsible pollution have led to increasingly serious environmental pollution. According to reports, there are 700,000 tons of dyes produced in the world every year, and 150,000 tons of dyes are produced in China every year. During its production and use, 10-15% of the dyes will be discharged into the environment with water. Dyes are biologically toxic and tertiary. Even if the concentration of dyes in wastewater is very low, the light transmittance of the water body will be reduced after being discharged into the water body, which...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 赵鸭美谢柳
Owner 浙江至美环境科技有限公司
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