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Biocatalysis preparation method of L-aminoacid derivates

A biocatalysis and amino acid technology, applied in the field of compound preparation, can solve the problems of various types of emissions, complex processes, high price, etc., and achieve the effects of short route, simple steps and resource saving.

Inactive Publication Date: 2011-06-15
ZHEJIANG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The former uses an asymmetric catalyst, which is expensive and difficult to recycle, and there are steps such as protection and deprotection in the process; the latter includes the Heine method, hydrolytic enzyme resolution method, etc., but each method requires multi-step reactions, the process is complicated, and the route Disadvantages such as long length and many types of emissions

Method used

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  • Biocatalysis preparation method of L-aminoacid derivates
  • Biocatalysis preparation method of L-aminoacid derivates
  • Biocatalysis preparation method of L-aminoacid derivates

Examples

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Embodiment 1

[0018] Embodiment 1 Preparation of high-purity L-phenylalanine derivatives (taking phenylalanine as an example)

[0019] In a 500ml shake flask, add 100mL medium, inoculate the bacteria Aeromonas sp. (CGMCC 2226) (Aeromonas) under sterile conditions, grow for 24 hours, centrifuge, pour out the supernatant, wash with water, and put back into the shake flask Add the same amount of racemate D, 50 mg of L-phenylalanine; adjust the pH to 7.3, ventilate the air, seal the seal, put it back in the temperature-controlled shaker, and shake it circularly. During the reaction, irregular (0 hour, 6 hours and 12 hours) one milliliter sample was taken out in stages, after ultrafiltration and dilution, it was injected into HPLC for analysis, and the reaction progress was tracked. With the Daise Crown Ether column, Crownpak CR(+), the analysis time was extended to 120 minutes. The results are shown in Figure 1, which shows the change process of the amount of each substance in the phenylalanin...

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Abstract

The invention provides a simple method for preparing high-purity L-amino acid by biocatalysis. A viable cell of bacteria Aeromonas sp. (preserving number CGMCC 2226) containing a plurality of enzymes can selectively oxidize D-type isomer in racemate amino acid under the condition of aerating air to leave unreacted high-purity L-amino acid. The method is reasonable in design without common derivative steps of protection in advance and protection once again in the prior method, is capable of directly and selectively transforming the D-type amino acid in the racemate, does not use metal catalysts, organic solvent or accessory ingredients during preparing process, and carries out reactions in neutral condition without chemical discharge except reproducible biomass, thereby belonging to green and environment-friendly production process. The method has the advantages of simple and short route, high efficiency, resource conservation and simple steps. The method is applicable to elimination of D-type antipode in more amino acids in different types and various mixtures possibly formed by the amino acids.

Description

technical field [0001] The invention belongs to the preparation method of the compound, a method for preparing highly enantiomerically pure L-amino acid by biocatalytically removing the D-type isomer in the racemate, and mainly relates to the biological production of derivatives of L-phenylalanine Catalytic preparation method. Background technique [0002] Non-protein amino acids, such as high-purity L-amino acid derivatives with different substituents, are important tools and basic raw materials for the development of modern new drugs. Non-protein amino acids with diverse structures have always been expensive in the world market, and the supply is in short supply. The ability to prepare non-protein amino acids is also a symbolic indicator of a country's new drug research and development capabilities. Compared with developed countries, my country lags far behind in this field. Due to the high added value of products and easy access to the market, the development of non-pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P41/00C12P13/04C12P13/22C12R1/01
Inventor 张子张郭红缨
Owner ZHEJIANG UNIV
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