Denitrification strain and application thereof

A strain and denitrification technology, applied in the field of environmental microorganisms, can solve problems such as long periods

Inactive Publication Date: 2019-11-12
博瑞德环境集团股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, there have been some reports of aerobic denitrification and nitrification performance strains at home and abroad, but heterotrophic nitrification-aerobic denitrification bacteria that can tolerate high concentrations of nitrate nitrogen at the same time are rarely reported. In the presence of nitrate nitrogen, it takes a long period to use normal biological treatment methods. Therefore, it is of great significance to obtain high-concentration nitrate-nitrogen tolerance degradation strains for the treatment of actual industrial wastewater.

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  • Denitrification strain and application thereof
  • Denitrification strain and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Isolation and Identification of Aeromonas PR-DN1 Strain

[0029] (A) It was isolated from an anoxic denitrification tank of a sewage treatment plant in Jiangbei, Nanjing by enrichment culture method, the specific steps are as follows.

[0030] (1) Preparation of medium: 100mL of nitrate nitrogen inorganic salt medium mixed system was loaded into a 250mL Erlenmeyer flask, including medium solution, inorganic salt trace element solution and potassium nitrate. Add 1mL of trace element solution to each 1L of medium, and sterilize at 120°C for 30min.

[0031] The medium solution is (g / L): 5g NaOOCCH 2 CH 2 COONa, 1 g KH 2 PO 4 , 1g K 2 HPO 4 ·3H 2 O, 0.2gMgSO 4 ·7H 2 O; trace element solution (g / L): 1g FeSO 4 ·7H 2 O, 1g MnSO 4 ·H 2 O, 0.25g Na 2 MoO 4 2H 2 O, 0.1g H 3 BO 4 , 0.25g CuCl 2 2H 2 O, 0.25g ZnCl 2 , 0.1g NH 4 ·VO 3 , 0.25g Co(NO 3 ) 2 ·6H 2 O, 0.1g NiSO 4 ·6H 2 O, dissolved in 900mL distilled water, add 5mL concentrated H 2 SO 4 , ...

Embodiment 2

[0049] Degradation rate of Aeromonas PR-DN1 to different concentrations of nitrate nitrogen and bacterial concentration (OD600 value)

[0050] At pH 7.0, temperature 30°C, and initial concentrations of nitrate nitrogen were 500mg / L, 1000mg / L, 1500mg / L, 2000mg / L, 2500mg / L, 3000mg / L, 3500mg / L, the PR-DN1 strain The culture was inoculated in 100 mL of the above-mentioned medium with potassium nitrate as the sole nitrogen source, cultured with shaking at 200 r / min for 48 hours, and then sampled.

[0051] The residual nitrate nitrogen content in the sample was measured by ultraviolet spectrophotometry, and the degradation rate was calculated. Taking the initial concentration of nitrate nitrogen as the abscissa, taking the degradation rate of nitrate nitrogen and OD600 value (cell concentration) as the ordinate, draw the curve of degradation and cell concentration, and the results are shown in figure 1 .

[0052] Depend on figure 1 It can be seen that Aeromonas PR-DN1 has a goo...

Embodiment 3

[0055] Degradation of Ammonia Nitrogen by Aeromonas PR-DN1

[0056] At pH 7.0, temperature 30°C, and initial ammonia nitrogen concentration of 1000 mg / L, the culture of PR-DN1 strain was inoculated into 100 mL of the above-mentioned medium with ammonium chloride as the sole nitrogen source, cultured with shaking at 200 r / min for 48 hours, ammonia nitrogen The residual amount is 1mg / L, and the removal rate of ammonia nitrogen reaches 99%.

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Abstract

The invention belongs to the technical field of environment microorganisms, and particularly relates to a denitrification strain and application thereof. The denitrification strain is Aeromonas sp. with the classification name of PR-DN1, the strain is preserved in China Center for Type Culture Collection (CCTCC) in April 15, 2019 with the preservation number of CCTCC NO:M2019255, and the denitrification strain is the efficient denitrification culture, namely Aeromonas sp. with heterotrophic nitrification-aerobic denitrification capacity, can efficiently degrade nitrate nitrogen in a nitrogen-containing solution, can tolerate the nitrate nitrogen concentration as high as 2500 mg / L, and is especially suitable for bio-treatment of high-concentration nitrogen-containing waste water.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a denitrification bacterial strain and application thereof. Background technique [0002] The main source of all nitrogen compounds on Earth is nitrogen. Human activities such as mining, industrial and agricultural nitrogen fixation, and excessive discharge of nitrogen-containing daily necessities into the environment seriously interfere with the natural nitrogen cycle and cause nitrogen pollution. All intermediate products of the nitrogen cycle have adverse effects on humans and the environment. Among them, ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen can induce eutrophication in water bodies, and nitrite nitrogen can also cause infant methemoglobinemia. [0003] The denitrification methods of sewage treatment include physical, chemical and biological methods. Biological denitrification technology is currently the most widely used d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 李丽严月根吴华明王丽梅宗春香郭娜
Owner 博瑞德环境集团股份有限公司
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