Dye decolored degradation treatment method for dye-containing waste water

A technology of dye wastewater and treatment method, which is applied in textile industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of increasing treatment costs and achieve the effect of reducing treatment costs.

Inactive Publication Date: 2006-07-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Using biological treatment technology, fungi such as Penicillium can be used to adsorb and decolorize various dyes in dye wastewater, such as: "Adsorption of Penicillium to Reactive Brilliant Blue KN-R" ["Journal of Applied and Environmental Biology" 2003, 9( 2).-179-182], reported the research results of Penicillium X5 on the adsorption of reactive brilliant blue KN-R, but the dye adsorbed on the hyphae was not decolorized and degraded, and still existed as harmful substances In the environment; the publication number is CN1654631A Chinese invention patent "a strain of yeast and its application", provides a strain of yeast in the decolorization liquid medium to the dye decolorization method, for the dye water or the presence of no nutrients Printing and dyeing wastewater cannot be decolorized, and adding nutrients to wastewater will increase the cost of treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The Penicillium P-1 that is cultivated on the inclined plane of the test tube is made into a spore suspension and inoculated in a liquid culture medium (containing 100g of potato boiled liquid in every liter of culture fluid, 5g of glucose, 2g of ammonium tartrate, natural pH, and a solvent of water), Under the condition that the temperature is 28°C, shake culture at 150rpm for 48h, take out the mycelium from the culture solution (reserved for use), put it into wastewater containing dyes or other organic poisons at a ratio of 10% (w / v), shake or stir for 5h, and the wastewater Other methods can be used to proceed to the next step of processing.

[0011] Put the mycelia of Penicillium P-1 with dye molecules absorbed from the wastewater back into the original culture solution, then add Enterobacter bacteria L-1 and Pseudomonas putida L-2, and treat for 5-96 hours. During this process, the synergistic effect of Penicillium P-1 and the mixed bacteria can quickly decolorize ...

Embodiment 2

[0014] Add Penicillium P-1 mycelia to 100mg / L Brilliant Blue KN-R, Reactive Red 3BE, Reactive Scarlet B-3G, and Brilliant Purple KN-B dye water respectively, shake and adsorb at 100rpm for 5h, and take the dye after fungal treatment Use a spectrophotometer to measure the OD value at the maximum absorption wavelength of the dye in the water supernatant, and use distilled water as a control to measure the decolorization rate to indicate the removal rate of the dye in the dye water. Removal rate (%)=(A-B) / A×100 (A—the OD value before water treatment, B—the OD value of the dyestuff after fungus treatment), and the chromaticity removal rate of the above-mentioned dye water reaches more than 90%.

[0015] Put the hyphae with dyestuffs into the original culture solution, and then add Aeromonas bacteria M10 strain, Enterobacter bacteria L-1, Pseudomonas putida L-2 mixed bacteria, at a temperature of 30-35 Under the condition of ℃, after standing for 5-96 hours, through the synergistic...

Embodiment 3

[0017] Add the mycelia of Penicillium P-1 to the dye bath wastewater with the highest concentration of dye in silk printing and dyeing factories with a chromaticity of 1000 times, oscillate and adsorb at 100rpm for 5 hours, take the supernatant of the dye bath wastewater treated by the fungus, and use a spectrophotometer to measure the dye The OD value at the maximum absorption wavelength, using distilled water as a control, measures the decolorization rate to indicate the removal rate of dyes in dye wastewater. Removal rate (%)=(A-B) / A×100 (A—OD value of wastewater before treatment, B—OD value of wastewater after fungus treatment). The color removal rate of dye bath wastewater can reach about 70%.

[0018] Put the hyphae with dyestuffs into the original culture solution, and then add Aeromonas bacteria M10 strain, Enterobacter bacteria L-1, Pseudomonas putida L-2 mixed bacteria, at a temperature of 30-35 Under the condition of ℃, after 48-72 hours of standing treatment, thro...

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PUM

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Abstract

The invention relates to a method of removing dye from waste water and decolorized degradation of dye molecule by biotechnology, belonging to environment protection technology field. Concrete processes are as follows: put fungi Penicillium sp.P-1 into dye water to adsorb dye for 2~5h, put said fungi with dye molecules into culture fluid and add bacteria Aeromonas sp.M10, etc, to said culture fluid to carry on decolorized degradation to dye molecules adsorbed on bacterial filaments. Two-type special microbe degrades multiple dye or other organic toxic molecules rapidly through co-metabolic reaction, co metabolism, etc, which could decrease the treatment cost of toxic material, avoid the danger of non-degradable organic dye molecules entering natural water body and accumulating and is characterized by appreciable economic benefits, environmental benefits and social benefits.

Description

technical field [0001] The invention relates to a treatment method for dye-containing wastewater, in particular to a treatment method for removing dye in dye-containing wastewater and decolorizing and degrading dye molecules by using biological technology, and belongs to the technical field of environmental protection. Background technique [0002] Due to the diversity and irregularity of the production varieties of dyestuffs and printing and dyeing enterprises, the nature of production wastewater is extremely unstable, and the types of dyes in wastewater change frequently and are difficult to degrade. It is difficult for microorganisms in conventional physical and chemical treatments to effectively degrade the ever-changing dye molecules. Even a small amount of dye molecules are still difficult to degrade after entering natural water bodies and accumulate in the environment, causing huge damage to the ecological environment of water bodies. In the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/30
Inventor 李蒙英
Owner SUZHOU UNIV
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