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Method of removing bloom-forming cyanobacteria by using Aeromonas sp.

An Aeromonas, water removal technology, applied in the field of using bacteria to degrade Microcystis aeruginosa, can solve the problems of staying, high-density algae pollution, secondary pollution by putting algicidal bacteria, etc., to avoid pollution and ensure universality. Effect

Active Publication Date: 2012-10-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on algae-dissolving bacteria mainly stays in the laboratory stage. Regarding the problem of genus, species, and strain diversity in dealing with algae pollution in practical applications, and the problem of high-density algae pollution during algae blooms, whether the introduction of algae-dissolving bacteria will bring The problem of secondary pollution is rarely involved, so there is still a distance from practical application, and there are no relevant reports so far

Method used

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  • Method of removing bloom-forming cyanobacteria by using Aeromonas sp.
  • Method of removing bloom-forming cyanobacteria by using Aeromonas sp.
  • Method of removing bloom-forming cyanobacteria by using Aeromonas sp.

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Embodiment 1

[0038] 1. Strain screening:

[0039] In this embodiment, the artificial pond of Jinan Botanical Garden is used as the source of alginolytic bacteria isolation. After enrichment and cultivation, multiple bacterial strains are obtained by coating and repeated marking. 10 6 cells / ml) in the water sample, the temperature is 25°C, the pH value is 6.5, the algicidal effect of each strain is investigated, and the algae density reduction rate is measured after treatment for 5 days, among which the highest algal density reduction rate is screened out The highly efficient algicidal strain DS-1. After physiological and biochemical analysis and 16S rRNA gene sequence analysis, the strain was identified as Aeromonas sp. and named Aeromonas sp.DS-1. Add 20% (w / w) glycerol to the liquid culture of the strain and store it for a long time below -20°C .

[0040] Bacterial culture adopts beef extract peptone liquid medium, and its formula is: beef extract 3g, peptone 10g, sodium chloride 5g, ...

Embodiment 2

[0043] Embodiment 2, utilize bacterial strain Aeromonas sp.DS-1 to remove anabaena flos-aquae (Anabaena flos-aquae)

[0044] The strain Aeromonas sp.DS-1 was inoculated in beef extract peptone liquid medium, cultured at 30°C and 200rpm for 16h, then spread 0.5mL culture solution on a 9cm plate, and cultured at 30°C for 48h, then The bacterial lawn was scraped off, and 27mg bacterial cells (wet weight) were added to 100ml containing Anabaena (5×10 6 Cells / ml) in the water sample, the temperature is 30 °C, the pH value is 6.5, after mixing, let it stand, and mix again every morning and evening, and the removal efficiency of Anabaena can reach 100% after 7 days of treatment.

Embodiment 3

[0045] Embodiment 3, utilize bacterial strain Aeromonas sp.DS-1 to remove nodularia spumigena (Nodularia spumigena)

[0046] The strain Aeromonas sp.DS-1 was inoculated in beef extract peptone liquid medium, cultured at 30°C and 200rpm for 16h, then spread 0.5mL culture solution on a 9cm plate, and cultured at 30°C for 48h, then The bacterial lawn was scraped off, and 33mg bacterial cells (wet weight) were added to 100ml containing nodular algae (1.1×10 6 Cells / ml) in the water sample, the temperature is 30°C, the pH value is 6.5, after mixing, let it stand still, and mix again every morning and evening, and the removal efficiency of nodular algae can reach 100% after 7 days of treatment.

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Abstract

The invention relates to a method of removing bloom-forming cyanobacteria by using Aeromonas sp., wherein, the strain of Aeromonas sp. DS-1 has been deposited in China Center for Type Culture Collection (CCTCC) on May 23rd, 2011 and assigned the accession number CCTCC No.M2011180. According to the invention, cells of the strain of Aeromonas sp. DS-1 are added into a water sample containing 106 to107 cells of cyanobacteria per milliliter at a temperature of 20 to 35 DEG C with a pH value being 6 to 7.5, and an obtained uniformly mixed mixture is allowed to stand and is subjected to treatment for 4 to 10 days; the method has an obvious and thorough effect in removing three representative cyanobacteria with great harm at present, i.e., Microcystis aeruginosa, Anabaena and Nodularia, and canbe used for controlling bloom-formation of cyanobacteria in real water bodies.

Description

technical field [0001] The invention relates to a method for removing bloom cyanobacteria by using Aeromonas, in particular to a method for degrading Microcystis aeruginosa by using bacteria, and belongs to the technical field of environmental protection. Background technique [0002] In recent years, the phenomenon of eutrophication in fresh water bodies in my country has been serious, and algae blooms have occurred frequently, seriously affecting the water quality and endangering the health of urban and rural residents in my country. In most water bodies where algal blooms occur in my country, the main dominant algae species are cyanobacteria, among which Microcystis aeruginosa is more common. The removal methods of algae in water generally include three categories: physical method, chemical method and biological method. Physical methods mainly include mechanical algae removal, air flotation algae removal, filtration algae removal and activated carbon adsorption algae rem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 李力刘玉梅王梦蕙
Owner SHANDONG UNIV
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